/*
* The MIT License (MIT)
*
* Copyright (c) 2007-2015 Broad Institute
*
* Permission is hereby granted, free of charge, to any person obtaining a copy
* of this software and associated documentation files (the "Software"), to deal
* in the Software without restriction, including without limitation the rights
* to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
* copies of the Software, and to permit persons to whom the Software is
* furnished to do so, subject to the following conditions:
*
* The above copyright notice and this permission notice shall be included in
* all copies or substantial portions of the Software.
*
*
* THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
* IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
* FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
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*/
package util;
import org.broad.igv.util.TestUtils;
import org.junit.Ignore;
import java.io.FileWriter;
import java.io.IOException;
import java.io.PrintWriter;
import java.util.Random;
/**
* Generates alignment files (.aligned format) for testing
*
* @author Jim Robinson
* @date 3/2/12
*/
@Ignore
public class AlignmentFileGenerator {
static Random RAND = new Random();
private static final int READ_PAIRED_FLAG = 0x1;
private static final int PROPER_PAIR_FLAG = 0x2;
private static final int READ_UNMAPPED_FLAG = 0x4;
private static final int MATE_UNMAPPED_FLAG = 0x8;
private static final int READ_STRAND_FLAG = 0x10;
private static final int MATE_STRAND_FLAG = 0x20;
private static final int FIRST_OF_PAIR_FLAG = 0x40;
private static final int SECOND_OF_PAIR_FLAG = 0x80;
private static final int NOT_PRIMARY_ALIGNMENT_FLAG = 0x100;
private static final int READ_FAILS_VENDOR_QUALITY_CHECK_FLAG = 0x200;
private static final int DUPLICATE_READ_FLAG = 0x400;
public static void main(String[] args) {
int t = 99;
System.out.println("READ_PAIRED_FLAG = " + (t & READ_PAIRED_FLAG));
t |= DUPLICATE_READ_FLAG;
System.out.println(t);
}
public static void generateTestFile() throws IOException {
String outputFile = TestUtils.DATA_DIR + "aligned/short_spread.aligned";
String chr = "chr1";
int averageDepth = 1000;
int avgReadSize = 100;
int readSigma = 5;
int interval = 1000;
boolean append = false;
int startPosition = 100; // arbitrary
PrintWriter pw = null;
try {
pw = new PrintWriter(new FileWriter(outputFile, append));
float averageReadsPerPosition = (float) averageDepth / avgReadSize;
for (int pos = startPosition; pos < startPosition + interval; pos++) {
int readSize = avgReadSize + (int) (readSigma * RAND.nextGaussian());
float avgReadCount = 2 * averageReadsPerPosition * RAND.nextFloat();
int readCount = (int) avgReadCount;
readCount += avgReadCount - readCount < RAND.nextFloat() ? 1 : 0;
while (readCount-- > 0) {
pw.println(chr + "\t" + pos + "\t" + (pos + readSize) + "\t+");
}
}
} finally {
if (pw != null) pw.close();
}
}
}