/* * The MIT License (MIT) * * Copyright (c) 2007-2015 Broad Institute * * Permission is hereby granted, free of charge, to any person obtaining a copy * of this software and associated documentation files (the "Software"), to deal * in the Software without restriction, including without limitation the rights * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell * copies of the Software, and to permit persons to whom the Software is * furnished to do so, subject to the following conditions: * * The above copyright notice and this permission notice shall be included in * all copies or substantial portions of the Software. * * * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN * THE SOFTWARE. */ package org.broad.igv.feature.genome; import org.broad.igv.util.TestUtils; import org.junit.BeforeClass; import org.junit.Test; import java.io.IOException; import static org.junit.Assert.assertEquals; /** * Created by IntelliJ IDEA. * User: jrobinso * Date: 8/7/11 * Time: 9:21 PM * To change this template use File | Settings | File Templates. */ public class FastaIndexedSequenceTest { static FastaIndexedSequence fastaSequence; @BeforeClass public static void setup() throws IOException { String path = "http://data.broadinstitute.org/igvdata/test/fasta/ci2_test.fa"; fastaSequence = new FastaIndexedSequence(path); } /** * Compare a direct read of sequence from a file vs a read from and indexed fasta for the same interval. * * @throws Exception */ @Test public void testReadSequence() throws Exception { String chr02qSeqPath = "http://data.broadinstitute.org/igvdata/test/fasta/"; String chr = "chr02q"; int start = 3531385; int end = 3531425; // TAATTTTTACGTCTTATTTAAACACATATAATGAATAGGT; Sequence igvSequence = new IGVSequence(chr02qSeqPath); byte[] expectedBytes = igvSequence.getSequence(chr, start, end); String expectedSequence = new String(expectedBytes); byte[] bytes = fastaSequence.getSequence(chr, start, end); String seq = new String(bytes); assertEquals(expectedSequence, seq); } @Test public void testReadEnd() throws Exception { String path = "http://data.broadinstitute.org/igvdata/test/fasta/ci2_test.fa"; String chr = "chr02q"; int chrLen = 8059593; int start = chrLen - 10; int end = chrLen + 10; byte[] bytes = fastaSequence.getSequence(chr, start, end); assertEquals(10, bytes.length); byte[] expectedSequence = "TTTTTCCCAG".getBytes(); for (int i = 0; i < 10; i++) { assertEquals(expectedSequence[i], bytes[i]); } } @Test public void testPaddedReference() throws Exception { String fasta = TestUtils.DATA_DIR + "fasta/ecoli_out.padded.fasta"; FastaUtils.createIndexFile(fasta, fasta + ".fai"); String expectedSequence = "atcaccattaccac******AAcggtgcgggctgacgcgtacaggaaacacagaaaaaag"; String chr = "NC_000913_bb"; int start = 240; int end = 300; FastaIndexedSequence sequence = new FastaIndexedSequence(fasta); byte[] bytes = sequence.getSequence(chr, start, end); assertEquals(expectedSequence, new String(bytes)); } // TODO -- add some assertions, what are we testing? @Test public void testPaddedReference2() throws Exception { String fasta = TestUtils.DATA_DIR + "fasta/ecoli_out.padded.fasta"; String chr = "NC_000913_bb"; int start = 0; int end = 5081; FastaIndexedSequence sequence = new FastaIndexedSequence(fasta); byte[] bytes = sequence.getSequence(chr, start, end); for (int i = 60; i < 100; i++) { } FastaIndexedSequence s = new FastaIndexedSequence(fasta); SequenceWrapper sequenceHelper = new SequenceWrapper(s); bytes = sequenceHelper.getSequence(chr, start, end); for (int i = 60; i < 100; i++) { // ?????? Not sure what to test here } } }