/* * The MIT License (MIT) * * Copyright (c) 2007-2015 Broad Institute * * Permission is hereby granted, free of charge, to any person obtaining a copy * of this software and associated documentation files (the "Software"), to deal * in the Software without restriction, including without limitation the rights * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell * copies of the Software, and to permit persons to whom the Software is * furnished to do so, subject to the following conditions: * * The above copyright notice and this permission notice shall be included in * all copies or substantial portions of the Software. * * * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN * THE SOFTWARE. */ package org.broad.igv.methyl; import org.broad.igv.Globals; import org.broad.igv.bbfile.BBFileReader; import org.broad.igv.bbfile.BedFeature; import org.broad.igv.bbfile.BigBedIterator; import org.broad.igv.feature.Strand; import org.broad.igv.feature.genome.Genome; import java.io.IOException; import java.util.*; import java.util.regex.Pattern; /** * Data source backe by Michael Ziller's custom BB format * * @author Jim Robinson * @date 4/19/12 */ public class BBMethylDataSource implements MethylDataSource { static Pattern percentPattern = Pattern.compile("%"); enum Type {ZILLER, USC} ; BBFileReader reader; Type type; /** * Map of chr name in genome definition -> chr name in BB file. Used for reverse-lookup during queries. */ Map<String, String> chrNameMap; public BBMethylDataSource(BBFileReader reader, Type type, Genome genome) throws IOException { this.reader = reader; this.type = type; init(genome); } public Iterator<MethylScore> query(String chr, int start, int end) { String tmp = chrNameMap.get(chr); String querySeq = tmp == null ? chr : tmp; BigBedIterator bedIterator = reader.getBigBedIterator(querySeq, start, chr, end, false); return new WrappedIterator(bedIterator, type); } private void init(Genome genome) { chrNameMap = new HashMap<String, String>(); if (genome != null) { Collection<String> seqNames = reader.getChromosomeNames(); if (seqNames != null) for (String seqName : seqNames) { String igvChr = genome.getCanonicalChrName(seqName); if (igvChr != null && !igvChr.equals(seqName)) { chrNameMap.put(igvChr, seqName); } } } } public static class WrappedIterator implements Iterator<MethylScore> { BigBedIterator bedIterator; Type type; public WrappedIterator(BigBedIterator bedIterator, Type type) { this.bedIterator = bedIterator; this.type = type; } public boolean hasNext() { return bedIterator.hasNext(); //To change body of implemented methods use File | Settings | File Templates. } public MethylScore next() { BedFeature feat = null; while (feat == null && bedIterator.hasNext()) { feat = bedIterator.next(); String[] restOfFields = feat.getRestOfFields(); MethylScore score = type == Type.ZILLER ? createZillerScore(feat, restOfFields) : createUSCScore(feat, restOfFields); return score; } return null; } private MethylScore createZillerScore(BedFeature feat, String[] restOfFields) { float percent; float count; String name = restOfFields[0]; if (name.contains("%")) { //'92%[51]' String[] tokens = percentPattern.split(name.replace("'", "").replace("[", "").replace("]", "")); percent = Float.parseFloat(tokens[0]); count = Float.parseFloat(tokens[1]); } else { // methylatedReads/totalreads String[] tokens = Globals.forwardSlashPattern.split(name.replace("'", "")); float methylatedReads = Float.parseFloat(tokens[0]); count = Float.parseFloat(tokens[1]); percent = (methylatedReads / count) * 100; } return new MethylScore(feat.getChromosome(), feat.getStartBase(), feat.getEndBase(), Strand.NONE, percent, (int) count); } private MethylScore createUSCScore(BedFeature feat, String[] restOfFields) { //String name = restOfFields[0]; //int score = Integer.parseInt(restOfFields[1]); char strandChar = restOfFields[2].charAt(0); Strand strand = strandChar == '+' ? Strand.POSITIVE : (strandChar == '-' ? Strand.NEGATIVE : Strand.NONE); float percentMethyl = Float.parseFloat(restOfFields[3]); int count = Integer.parseInt(restOfFields[4]); return new MethylScore(feat.getChromosome(), feat.getStartBase(), feat.getEndBase(), strand, percentMethyl, count); } public void remove() { //To change body of implemented methods use File | Settings | File Templates. } } }