/* * The MIT License (MIT) * * Copyright (c) 2007-2015 Broad Institute * * Permission is hereby granted, free of charge, to any person obtaining a copy * of this software and associated documentation files (the "Software"), to deal * in the Software without restriction, including without limitation the rights * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell * copies of the Software, and to permit persons to whom the Software is * furnished to do so, subject to the following conditions: * * The above copyright notice and this permission notice shall be included in * all copies or substantial portions of the Software. * * * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN * THE SOFTWARE. */ package org.broad.igv.tools.converters; import org.broad.igv.tools.parsers.DataConsumer; import org.broad.igv.tools.parsers.ToolsWiggleParser; import org.broad.igv.track.TrackType; import java.io.BufferedWriter; import java.io.FileWriter; import java.io.IOException; import java.io.PrintWriter; /** * Converts a "wig" file to a "bed" file by applying a threshold * * @author jrobinso * @date Jan 20, 2011 */ public class WigToBed implements DataConsumer { PrintWriter bedWriter; double lowerThreshold = 0.1; double higherThreshold = 0.3; String chr = null; int featureStart = -1; int featureEnd = -1; String type; private float score; static String upperName = "upper"; static String lowerName = "lower"; public static void main(String[] args) throws IOException { String input = args[0]; //"/Users/jrobinso/Sigma/566.wgs.bam.large_isize.wig"; WigToBed wigToBed = new WigToBed(input, .17f, .55f); ToolsWiggleParser parser = new ToolsWiggleParser(input, wigToBed, null); parser.parse(); } public static void run(String inputFile, float hetThreshold, float homThreshold) throws IOException { lowerName = String.valueOf((int) (hetThreshold * 10)); upperName = String.valueOf((int) (homThreshold * 10)); int len = inputFile.length(); String outputFile = inputFile.substring(0, len - 4) + ".bed"; WigToBed wigToBed = new WigToBed(outputFile, hetThreshold, homThreshold); ToolsWiggleParser parser = new ToolsWiggleParser(inputFile, wigToBed, null); parser.parse(); } public WigToBed(String bedFile, float lowerThreshold, float higherThreshold) { this.lowerThreshold = lowerThreshold; this.higherThreshold = higherThreshold; try { bedWriter = new PrintWriter(new BufferedWriter(new FileWriter(bedFile))); } catch (IOException e) { e.printStackTrace(); //To change body of catch statement use File | Settings | File Templates. } } public void addData(String chr, int start, int end, float[] data, String name) { if (featureStart >= 0) { // Feature in progress if (start > featureEnd || data[0] < lowerThreshold) { writeCurrentFeature(); featureStart = -1; score = 0f; } featureEnd = end; } if (featureStart >= 0) { if (data[0] > higherThreshold) { type = String.valueOf(higherThreshold); score = Math.max(score, data[0]); } else if (data[0] < lowerThreshold) { writeCurrentFeature(); featureStart = -1; score = 0f; } } else { if (data[0] > lowerThreshold) { featureStart = start; featureEnd = end; this.chr = chr; score = Math.max(score, data[0]); type = data[0] > higherThreshold ? String.valueOf(higherThreshold) : String.valueOf(lowerThreshold); } } } private void writeCurrentFeature() { bedWriter.println(this.chr + "\t" + featureStart + "\t" + featureEnd + "\t" + type); featureStart = -1; } public void parsingComplete() { if (featureStart >= 0) writeCurrentFeature(); bedWriter.close(); } public void setTrackParameters(TrackType trackType, String trackLine, String[] trackNames) { //To change body of implemented methods use File | Settings | File Templates. } public void setTrackParameters(TrackType trackType, String trackLine, String[] trackNames, boolean b) { //To change body of implemented methods use File | Settings | File Templates. } /** * Set a tolerance for "sortedness" of the data. A start position can be less than * the immediately previous start position by this amount. This is needed for * chip-seq processing where the start position of an alignment can be artificially * extended post sorting. */ public void setSortTolerance(int tolerance) { //To change body of implemented methods use File | Settings | File Templates. } public void setAttribute(String key, String value) { //To change body of implemented methods use File | Settings | File Templates. } public void setType(String type) { //To change body of implemented methods use File | Settings | File Templates. } }