/*
* The MIT License (MIT)
*
* Copyright (c) 2007-2015 Broad Institute
*
* Permission is hereby granted, free of charge, to any person obtaining a copy
* of this software and associated documentation files (the "Software"), to deal
* in the Software without restriction, including without limitation the rights
* to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
* copies of the Software, and to permit persons to whom the Software is
* furnished to do so, subject to the following conditions:
*
* The above copyright notice and this permission notice shall be included in
* all copies or substantial portions of the Software.
*
*
* THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
* IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
* FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
* AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
* LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
* OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN
* THE SOFTWARE.
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package org.broad.igv.feature.genome;
import org.broad.igv.AbstractHeadlessTest;
import org.broad.igv.prefs.IGVPreferences;
import org.broad.igv.prefs.PreferencesManager;
import org.broad.igv.util.HttpUtils;
import org.junit.After;
import org.junit.BeforeClass;
import org.junit.Test;
import java.io.IOException;
import java.util.HashSet;
import java.util.Set;
import static org.junit.Assert.assertEquals;
import static org.junit.Assert.assertFalse;
/**
* @author jrobinso
*/
public class SequenceWrapperTest extends AbstractHeadlessTest{
static String seqPath = "http://igvdata.broadinstitute.org/genomes/seq/hg18/";
static IGVPreferences preferenceManager;
static SequenceWrapper helper;
public SequenceWrapperTest() {
}
@BeforeClass
public static void setUpClass() throws Exception {
AbstractHeadlessTest.setUpClass();
preferenceManager = PreferencesManager.getPreferences();
String tmp = SequenceWrapper.checkSequenceURL(seqPath);
helper = new SequenceWrapper(new IGVSequence(tmp));
}
@After
public void tearDown() throws Exception{
super.tearDown();
SequenceWrapper.setCacheSequences(true);
}
/**
* Test cached vs uncached sequence reads.
*/
@Test
public void testCache() {
try {
String chr = "chr7";
int start = 55054464;
int end = start + 10000;
byte[] cachedSeq = helper.getSequence(chr, start, end);
SequenceWrapper.setCacheSequences(false);
byte[] uncachedSeq = helper.getSequence(chr, start, end);
assertEquals(uncachedSeq.length, cachedSeq.length);
for (int i = 0; i < cachedSeq.length; i++) {
assertEquals("i=" + i, uncachedSeq[i], cachedSeq[i]);
}
} finally {
SequenceWrapper.setCacheSequences(true);
}
}
@Test
public void testNonCloudGenome() throws IOException {
String chr = "scaffold_v2_26164";
int start = 5;
int end = 10;
String expSequence = "ATTGC";
String tmp = SequenceWrapper.checkSequenceURL("http://data.broadinstitute.org/igvdata/annotations/seq/spur_2.1/");
SequenceWrapper helper = new SequenceWrapper(new IGVSequence(tmp));
byte[] seq = helper.getSequence(chr, start, end);
assertEquals(expSequence, new String(seq));
}
/**
* Test known sequence (start of EGFR).
*/
@Test
public void readEGFRSequence() {
String chr = "chr7";
int start = 55054464;
int end = start + 20;
String expSequence = "ATGCGACCCTCCGGGACGGC";
byte[] seq = helper.getSequence(chr, start, end);
assertEquals(expSequence, new String(seq));
}
/**
* Test fetching sequence using the "Range service" for 3 URL patterns: cloudfront, s3 storage, and the
* Broad server.
*/
@Test
public void testRangeService() {
try {
HttpUtils.disableByteRange(true);
seqPath = "http://igvdata.broadinstitute.org/genomes/seq/hg18/";
readEGFRSequence();
seqPath = "http://igv.broadinstitute.org/genomes/seq/hg18/";
readEGFRSequence();
seqPath = "http://data.broadinstitute.org/igvdata/annotations/seq/hg18/";
readEGFRSequence();
} finally {
HttpUtils.disableByteRange(false);
}
}
@Test
public void testGetKeyUniqueness() throws Exception{
int count = 0;
int maxNumTiles = 100;
Set<String> keys = new HashSet<String>();
for(String chr: genome.getAllChromosomeNames()){
for(int tNo=0; tNo < maxNumTiles; tNo++){
String key = SequenceWrapper.getKey(chr, tNo);
assertFalse("Key not unique: " + key, keys.contains(key));
keys.add(key);
count++;
}
}
assertEquals(count, keys.size());
}
}