/* * The MIT License (MIT) * * Copyright (c) 2007-2015 Broad Institute * * Permission is hereby granted, free of charge, to any person obtaining a copy * of this software and associated documentation files (the "Software"), to deal * in the Software without restriction, including without limitation the rights * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell * copies of the Software, and to permit persons to whom the Software is * furnished to do so, subject to the following conditions: * * The above copyright notice and this permission notice shall be included in * all copies or substantial portions of the Software. * * * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN * THE SOFTWARE. */ package org.broad.igv.data.cufflinks; import org.apache.log4j.Logger; import org.broad.igv.feature.Locus; import org.broad.igv.util.ParsingUtils; /** * Codec for Cufflinks FPKM files, extension fpkm_tracking * * @author jacob * @date 2013-Apr-18 */ public class FPKMTrackingCodec extends CufflinksCodec<FPKMValue>{ private static Logger log = Logger.getLogger(FPKMTrackingCodec.class); int geneColumn = 4; int locusColumn = 6; static final int startfpkmCol = 9; static final int colsPerSample = 4; private int numSamples = 1; public FPKMTrackingCodec(String path){ super(FPKMValue.class, path); } @Override public Object readHeader(String[] tokens) { for(int i=0; i<tokens.length; i++) { final String tk = tokens[i]; if(tk.equals("locus")) locusColumn = i; else if(tk.equals("gene_short_name")) geneColumn = i; } numSamples = (tokens.length - startfpkmCol) / colsPerSample; return tokens; } @Override public FPKMValue decode(String line) { return decode(ParsingUtils.TAB_PATTERN.split(line)); } //@Override public FPKMValue decode(String[] tokens) { //Skip header line if (tokens[0].equalsIgnoreCase("tracking_id") || tokens[geneColumn].equalsIgnoreCase("gene_short_name")) { return null; } if (tokens.length >= (startfpkmCol + numSamples*colsPerSample)) { String locusString = tokens[locusColumn]; if (locusString == null) return null; Locus locus = Locus.fromString(locusString); if(locus == null || locus.getChr() == null) return null; String gene = tokens[geneColumn]; float[] fpkm = new float[numSamples]; float[] confLo = new float[numSamples]; float[] confHi = new float[numSamples]; for(int sampNum = 0; sampNum < numSamples; sampNum++){ int startCol = startfpkmCol + sampNum*colsPerSample; fpkm[sampNum] = Float.parseFloat(tokens[startCol]); confLo[sampNum] = Float.parseFloat(tokens[startCol+1]); confHi[sampNum] = Float.parseFloat(tokens[startCol+2]); } return new FPKMValue(locus.getChr(), locus.getStart() - 1, locus.getEnd(), gene, fpkm, confLo, confHi); } else { log.info("Unexpected # of columns. Expected at least 12, found " + tokens.length); return null; } } public int getNumSamples() { return numSamples; } @Override public boolean canDecode(String path) { String fn = path.toLowerCase(); if(fn.endsWith(".gz")) fn = fn.substring(0, fn.length()-3); return fn.endsWith("fpkm_tracking"); } }