/* * The MIT License (MIT) * * Copyright (c) 2007-2015 Broad Institute * * Permission is hereby granted, free of charge, to any person obtaining a copy * of this software and associated documentation files (the "Software"), to deal * in the Software without restriction, including without limitation the rights * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell * copies of the Software, and to permit persons to whom the Software is * furnished to do so, subject to the following conditions: * * The above copyright notice and this permission notice shall be included in * all copies or substantial portions of the Software. * * * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN * THE SOFTWARE. */ package org.broad.igv.data.cufflinks; import org.apache.log4j.Logger; import org.broad.igv.feature.Locus; import org.broad.igv.util.ParsingUtils; /** * Codec for cufflinks .exp_diff files * @author jacob * @date 2013-Apr-18 */ public class ExpDiffCodec extends CufflinksCodec<ExpDiffValue>{ private static Logger log = Logger.getLogger(ExpDiffCodec.class); int geneColumn = 1; int locusColumn = 2; int xColumn = 6; int yColumn = 7; int logRatioColumn = 8; int sigColumn = 12; public ExpDiffCodec(String path) { super(ExpDiffValue.class, path); } @Override public Object readHeader(String[] tokens) { for (int i = 0; i < tokens.length; i++) { final String tk = tokens[i]; if (tk.equals("locus")) locusColumn = i; else if (tk.equals("gene")) geneColumn = i; else if (tk.equals("value_1")) xColumn = i; else if (tk.equals("value_2")) yColumn = i; else if (tk.startsWith("log2(")) logRatioColumn = i; else if (tk.equals("significant")) sigColumn = i; } return tokens; } //@Override public ExpDiffValue decode(String[] tokens) { //Skip header line if (tokens[0].equalsIgnoreCase("test_id") || tokens[geneColumn].equalsIgnoreCase("gene_id")) { return null; } if (tokens.length >= sigColumn) { String locusString = tokens[locusColumn]; if (locusString == null) return null; Locus locus = Locus.fromString(locusString); if (locus == null || locus.getChr() == null) return null; String logRatioStr = tokens[logRatioColumn]; float logRatio = Float.parseFloat(logRatioStr); if (Float.isInfinite(logRatio) || Float.isNaN(logRatio)) { log.info("LogRatio " + logRatioStr + " cannot be parsed as a float"); logRatio = Float.NaN; } float fpkmX = Float.parseFloat(tokens[xColumn]); float fpkmY = Float.parseFloat(tokens[yColumn]); String gene = tokens[geneColumn]; String significant = tokens[sigColumn]; return new ExpDiffValue(locus.getChr(), locus.getStart() - 1, locus.getEnd(), gene, logRatio, fpkmX, fpkmY, significant); } else { log.info("Unexpected # of columns. Expected at least 12, found " + tokens.length); return null; } } @Override public ExpDiffValue decode(String line) { return decode(ParsingUtils.TAB_PATTERN.split(line)); } @Override public boolean canDecode(String path) { String s = path.toLowerCase(); if(s.endsWith(".gz")) s = s.substring(0, s.length()-3); return s.endsWith("gene_exp.diff") || s.endsWith("cds_exp.diff"); } }