/*
* The MIT License (MIT)
*
* Copyright (c) 2016 University of California San Diego
* Author: Jim Robinson
*
* Permission is hereby granted, free of charge, to any person obtaining a copy
* of this software and associated documentation files (the "Software"), to deal
* in the Software without restriction, including without limitation the rights
* to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
* copies of the Software, and to permit persons to whom the Software is
* furnished to do so, subject to the following conditions:
*
* The above copyright notice and this permission notice shall be included in
* all copies or substantial portions of the Software.
*
*
* THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
* IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
* FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
* AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
* LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
* OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN
* THE SOFTWARE.
*/
package org.broad.igv.tools.sort;
import htsjdk.samtools.*;
import htsjdk.samtools.util.CloserUtil;
import org.apache.log4j.Logger;
import org.broad.igv.ui.IGV;
import java.io.File;
import java.io.IOException;
import java.util.Comparator;
public class BAMSorter implements Sorter {
private static Logger log = Logger.getLogger(BAMSorter.class);
File inputFile;
File outputFile;
private File tmpDir;
private int maxRecords = -1;
public BAMSorter(File inputFile, File outputFile) {
this.inputFile = inputFile;
this.outputFile = outputFile;
}
@Override
public void run() throws IOException {
SamReaderFactory.setDefaultValidationStringency(ValidationStringency.SILENT);
final SamReader reader = SamReaderFactory.makeDefault().open(inputFile);
reader.getFileHeader().setSortOrder(SAMFileHeader.SortOrder.coordinate);
SAMFileWriterFactory samFileWriterFactory = new SAMFileWriterFactory();
if(this.tmpDir != null) {
samFileWriterFactory.setTempDirectory(this.tmpDir);
}
if(this.maxRecords > 0) {
samFileWriterFactory.setMaxRecordsInRam(this.maxRecords);
}
final SAMFileWriter writer = samFileWriterFactory.makeSAMOrBAMWriter(reader.getFileHeader(), false, outputFile);
int count = 0;
for (final SAMRecord rec : reader) {
if(++count % 100000 == 0) {
if(IGV.hasInstance()) {
System.out.println("" + count + " records processed"); // GUI
}
else {
log.info("" + count + " records processed"); // Command line
}
}
writer.addAlignment(rec);
}
CloserUtil.close(reader);
writer.close();
}
@Override
public void setTmpDir(File tmpDir) {
this.tmpDir = tmpDir;
}
@Override
public void setMaxRecords(int maxRecords) {
this.maxRecords = maxRecords;
}
@Override
public void setComparator(Comparator<SortableRecord> comparator) {
throw new RuntimeException("Not implemented");
}
}