/* * The MIT License (MIT) * * Copyright (c) 2007-2015 Broad Institute * * Permission is hereby granted, free of charge, to any person obtaining a copy * of this software and associated documentation files (the "Software"), to deal * in the Software without restriction, including without limitation the rights * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell * copies of the Software, and to permit persons to whom the Software is * furnished to do so, subject to the following conditions: * * The above copyright notice and this permission notice shall be included in * all copies or substantial portions of the Software. * * * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN * THE SOFTWARE. */ package org.broad.igv.methyl; import junit.framework.Assert; import org.broad.igv.bbfile.BBFileReader; import org.broad.igv.feature.Strand; import org.broad.igv.feature.genome.Genome; import org.broad.igv.util.TestUtils; import org.junit.Test; import java.util.ArrayList; import java.util.Iterator; import java.util.List; import static junit.framework.Assert.assertTrue; import static org.junit.Assert.assertEquals; /** * Test of data source for Michael Ziller's hacked up bigbed format for methylation scores. * * @author Jim Robinson * @date 4/19/12 */ public class BBMethylDataSourceTest { @Test public void testUscQuery() throws Exception { // Test data // chr11 10001654 10001656 . 1000 . 100.000 7 // chr11 10001667 10001669 . 833 . 83.333 6 // chr11 10001752 10001754 . 625 . 62.500 8 int[] expectedStarts = {10001654, 10001667, 10001752}; int[] expectedEnds = {10001656, 10001669, 10001754}; float[] expectedPercents = {100.0f, 83.333f, 62.500f}; int[] expectedCounts = {7, 6, 8}; String testFile = TestUtils.DATA_DIR + "/methylation/usc/jhu-usc.edu_UCEC.IlluminaHiSeq_WGBS.Level_3.1.0.0.hg18.SAMPLE.bb"; Genome genome = null; // Don't need a genome for this test String chr = "chr11"; int start = 10001654; int end = 10001754; BBFileReader reader = new BBFileReader(testFile); MethylDataSource source = new BBMethylDataSource(reader, BBMethylDataSource.Type.USC, genome); Iterator<MethylScore> iter = source.query(chr, start, end); int i = 0; while (iter.hasNext()) { MethylScore ms = iter.next(); assertEquals(chr, ms.getChr()); assertEquals(expectedStarts[i], ms.getStart()); assertEquals(expectedEnds[i], ms.getEnd()); assertEquals(expectedPercents[i], ms.getScore(), .000001); assertEquals(expectedCounts[i], ms.getCount()); assertEquals(Strand.NONE, ms.getStrand()); i++; } assertEquals(3, i); } //@Test public void testZillerQuery() throws Exception { String testFile = "path-to-test-file.bb"; String chr = "chr7"; int start = 26133475; int end = 27333475; Genome genome = null; BBFileReader reader = new BBFileReader(testFile); MethylDataSource source = new BBMethylDataSource(reader, BBMethylDataSource.Type.ZILLER, genome); Iterator<MethylScore> iter = source.query(chr, start, end); // Should find at least 1 score. assertTrue(iter.hasNext()); while (iter.hasNext()) { MethylScore score = iter.next(); assertTrue(score.getEnd() >= start && score.getStart() <= end); assertTrue(score.getScore() >= 0 && score.getScore() <= 100); } } // @Test public void testCacheQuery() throws Exception { String testFile = "path-to-test-file.bb"; String chr = "chr7"; int start = 26133475; int end = 27333475; Genome genome = null; BBFileReader reader = new BBFileReader(testFile); MethylDataSource source = new BBMethylDataSource(reader, BBMethylDataSource.Type.ZILLER, genome); Iterator<MethylScore> iter = source.query(chr, start, end); List<MethylScore> nonCachedScores = new ArrayList<MethylScore>(); while (iter.hasNext()) { nonCachedScores.add(iter.next()); } int maxTileCount = 100000; int tileSize = 100; MethylDataSource cachedSource = new CachingMethylSource(new BBMethylDataSource(reader, BBMethylDataSource.Type.ZILLER, genome), maxTileCount, tileSize); Iterator<MethylScore> iter2 = cachedSource.query(chr, start, end); List<MethylScore> cachedScores = new ArrayList<MethylScore>(); while (iter2.hasNext()) { cachedScores.add(iter2.next()); } assertTrue(cachedScores.size() > 0); Assert.assertEquals(cachedScores.size(), nonCachedScores.size()); for (int i = 0; i < cachedScores.size(); i++) { MethylScore cScore = cachedScores.get(i); MethylScore score = nonCachedScores.get(i); Assert.assertEquals(cScore.getStart(), score.getStart()); Assert.assertEquals(cScore.getChr(), score.getChr()); } } }