/* * Eoulsan development code * * This code may be freely distributed and modified under the * terms of the GNU Lesser General Public License version 2.1 or * later and CeCILL-C. This should be distributed with the code. * If you do not have a copy, see: * * http://www.gnu.org/licenses/lgpl-2.1.txt * http://www.cecill.info/licences/Licence_CeCILL-C_V1-en.txt * * Copyright for this code is held jointly by the Genomic platform * of the Institut de Biologie de l'École normale supérieure and * the individual authors. These should be listed in @author doc * comments. * * For more information on the Eoulsan project and its aims, * or to join the Eoulsan Google group, visit the home page * at: * * http://outils.genomique.biologie.ens.fr/eoulsan * */ package fr.ens.biologie.genomique.eoulsan.modules.generators; import static com.google.common.base.Preconditions.checkNotNull; import static fr.ens.biologie.genomique.eoulsan.data.DataFormats.GENOME_DESC_TXT; import static fr.ens.biologie.genomique.eoulsan.data.DataFormats.GENOME_FASTA; import static fr.ens.biologie.genomique.eoulsan.modules.mapping.AbstractReadsMapperModule.MAPPER_FLAVOR_PARAMETER_NAME; import static fr.ens.biologie.genomique.eoulsan.modules.mapping.AbstractReadsMapperModule.MAPPER_VERSION_PARAMETER_NAME; import java.io.IOException; import java.util.Collections; import java.util.Map; import java.util.Set; import fr.ens.biologie.genomique.eoulsan.EoulsanException; import fr.ens.biologie.genomique.eoulsan.Globals; import fr.ens.biologie.genomique.eoulsan.bio.GenomeDescription; import fr.ens.biologie.genomique.eoulsan.bio.readsmappers.SequenceReadsMapper; import fr.ens.biologie.genomique.eoulsan.bio.readsmappers.SequenceReadsMapperService; import fr.ens.biologie.genomique.eoulsan.core.InputPorts; import fr.ens.biologie.genomique.eoulsan.core.InputPortsBuilder; import fr.ens.biologie.genomique.eoulsan.core.Modules; import fr.ens.biologie.genomique.eoulsan.core.OutputPorts; import fr.ens.biologie.genomique.eoulsan.core.OutputPortsBuilder; import fr.ens.biologie.genomique.eoulsan.core.Parameter; import fr.ens.biologie.genomique.eoulsan.core.Step; import fr.ens.biologie.genomique.eoulsan.core.StepConfigurationContext; import fr.ens.biologie.genomique.eoulsan.core.TaskContext; import fr.ens.biologie.genomique.eoulsan.core.TaskResult; import fr.ens.biologie.genomique.eoulsan.core.TaskStatus; import fr.ens.biologie.genomique.eoulsan.core.Version; import fr.ens.biologie.genomique.eoulsan.data.Data; import fr.ens.biologie.genomique.eoulsan.data.DataFile; import fr.ens.biologie.genomique.eoulsan.modules.AbstractModule; import fr.ens.biologie.genomique.eoulsan.modules.mapping.AbstractFilterAndMapReadsModule; import fr.ens.biologie.genomique.eoulsan.modules.mapping.AbstractReadsMapperModule; /** * This class define a module that generate a genome mapper index. * @since 1.0 * @author Laurent Jourdren */ public class GenomeMapperIndexGeneratorModule extends AbstractModule { public static final String MODULE_NAME = "genericindexgenerator"; private SequenceReadsMapper mapper; @Override public String getName() { return MODULE_NAME; } @Override public String getDescription() { return "Generate Mapper index"; } @Override public Version getVersion() { return Globals.APP_VERSION; } @Override public InputPorts getInputPorts() { return new InputPortsBuilder().addPort("genome", GENOME_FASTA) .addPort("genomedescription", GENOME_DESC_TXT).create(); } @Override public OutputPorts getOutputPorts() { return OutputPortsBuilder.singleOutputPort(this.mapper.getArchiveFormat()); } @Override public void configure(final StepConfigurationContext context, final Set<Parameter> stepParameters) throws EoulsanException { if (stepParameters == null) { throw new EoulsanException( "No parameters set in " + getName() + " generator"); } for (Parameter p : stepParameters) { switch (p.getName()) { // TODO replace with AbstractReadsMapperStep.MAPPER_NAME_PARAMETER_NAME ? case "mappername": final String mapperName = p.getStringValue(); this.mapper = SequenceReadsMapperService.getInstance().newService(mapperName); if (this.mapper == null) { Modules.badParameterValue(context, p, "Unknown mapper"); } break; default: Modules.unknownParameter(context, p); } } } /** * Set the version and the flavor of a mapper. * @param mapper mapper to configure * @param context the context of the task * @throws EoulsanException if more than one mapping step require this * generator */ static void searchMapperVersionAndFlavor(final SequenceReadsMapper mapper, final TaskContext context) throws EoulsanException { int count = 0; String version = null; String flavor = null; for (Step step : context.getWorkflow().getSteps()) { if (AbstractReadsMapperModule.MODULE_NAME.equals(step.getModuleName()) || AbstractFilterAndMapReadsModule.MODULE_NAME .equals(step.getModuleName())) { for (Parameter p : step.getParameters()) { switch (p.getName()) { case MAPPER_VERSION_PARAMETER_NAME: version = p.getStringValue(); break; case MAPPER_FLAVOR_PARAMETER_NAME: flavor = p.getStringValue(); break; default: break; } } count++; } } if (count > 1) { throw new EoulsanException( "Found more than one mapping step in the workflow"); } // Set the version and the flavor to use mapper.setMapperVersionToUse(version); mapper.setMapperFlavorToUse(flavor); } /** * Execute the indexer. * @param mapper Mapper to use for the index generator * @param context Eoulsan context * @param additionalArguments additional indexer arguments * @param additionalDescription additional indexer arguments description * @param threadCount the number of thread to use */ static void execute(final SequenceReadsMapper mapper, final TaskContext context, final String additionalArguments, final Map<String, String> additionalDescription, final int threadCount) throws IOException, EoulsanException { checkNotNull(mapper, "mapper argument cannot be null"); checkNotNull(context, "context argument cannot be null"); // Set default value for arguments if needed final String args = additionalArguments != null ? additionalArguments : ""; // Set default value for descriptions if needed final Map<String, String> descriptions; if (additionalDescription != null) { descriptions = additionalDescription; } else { descriptions = Collections.emptyMap(); } // Get input and output data final Data genomeData = context.getInputData(GENOME_FASTA); final Data genomeDescData = context.getInputData(GENOME_DESC_TXT); final Data outData = context.getOutputData(mapper.getArchiveFormat(), genomeData); // Get the genome DataFile final DataFile genomeDataFile = genomeData.getDataFile(); // Get the genome description DataFile final DataFile descDataFile = genomeDescData.getDataFile(); final GenomeDescription desc = GenomeDescription.load(descDataFile.open()); // Get the output DataFile final DataFile mapperIndexDataFile = outData.getDataFile(); // Set the version and flavor searchMapperVersionAndFlavor(mapper, context); // Set mapper temporary directory mapper.setTempDirectory(context.getLocalTempDirectory()); // Set mapper executable temporary directory mapper.setExecutablesTempDirectory( context.getSettings().getExecutablesTempDirectoryFile()); // Set the number of thread to use final int threads = threadCount < 1 ? Runtime.getRuntime().availableProcessors() : threadCount; mapper.setThreadsNumber(threads); // Create indexer final GenomeMapperIndexer indexer = new GenomeMapperIndexer(mapper, args, descriptions); // Create index indexer.createIndex(genomeDataFile, desc, mapperIndexDataFile); } @Override public TaskResult execute(final TaskContext context, final TaskStatus status) { try { status .setProgressMessage(this.mapper.getMapperName() + " index creation"); // Create the index execute(this.mapper, context, null, null, 0); } catch (IOException | EoulsanException e) { return status.createTaskResult(e); } return status.createTaskResult(); } }