/* * Eoulsan development code * * This code may be freely distributed and modified under the * terms of the GNU Lesser General Public License version 2.1 or * later and CeCILL-C. This should be distributed with the code. * If you do not have a copy, see: * * http://www.gnu.org/licenses/lgpl-2.1.txt * http://www.cecill.info/licences/Licence_CeCILL-C_V1-en.txt * * Copyright for this code is held jointly by the Genomic platform * of the Institut de Biologie de l'École normale supérieure and * the individual authors. These should be listed in @author doc * comments. * * For more information on the Eoulsan project and its aims, * or to join the Eoulsan Google group, visit the home page * at: * * http://outils.genomique.biologie.ens.fr/eoulsan * */ package fr.ens.biologie.genomique.eoulsan.bio.alignmentsfilters; import htsjdk.samtools.SAMRecord; import java.util.ArrayList; import java.util.List; import fr.ens.biologie.genomique.eoulsan.EoulsanException; /** * This alignments filter keep a given number of the first alignments for a * read. * @since 1.2 * @author Claire Wallon */ public class KeepNumberMatchReadAlignmentsFilter extends AbstractReadAlignmentsFilter { public static final String FILTER_NAME = "keepnumbermatch"; private int numberMatch = -1; @Override public String getName() { return FILTER_NAME; } @Override public String getDescription() { return "This filter allows to keep a given number of alignments of a read."; } @Override public void setParameter(final String key, final String value) throws EoulsanException { if (key == null || value == null) { return; } if ("threshold".equals(key.trim())) { try { this.numberMatch = Integer.parseInt(value.trim()); } catch (NumberFormatException e) { return; } if (this.numberMatch < 0) { throw new EoulsanException( "Invalid number of match to keep: " + this.numberMatch); } } else { throw new EoulsanException( "Unknown parameter for " + getName() + " alignments filter: " + key); } } @Override public void init() { if (this.numberMatch < 0) { throw new IllegalArgumentException( "The number of match to keep is not set for " + getName() + " alignments filter."); } } @Override public void filterReadAlignments(final List<SAMRecord> records) { if (records == null || records.isEmpty()) { return; } List<SAMRecord> recordsToKeep = new ArrayList<>(); int cptRecords = 0; int nbRecordsToKeep; // single-end mode if (!records.get(0).getReadPairedFlag()) { nbRecordsToKeep = this.numberMatch; } // paired-end mode else { nbRecordsToKeep = this.numberMatch * 2; } if (records.size() > nbRecordsToKeep) { while (cptRecords < nbRecordsToKeep) { recordsToKeep.add(records.get(cptRecords)); cptRecords++; } records.clear(); records.addAll(recordsToKeep); } } @Override public String toString() { return this.getClass().getSimpleName() + "{name=" + getName() + ", numberMatch=" + this.numberMatch + "}"; } }