/*
* Eoulsan development code
*
* This code may be freely distributed and modified under the
* terms of the GNU Lesser General Public License version 2.1 or
* later and CeCILL-C. This should be distributed with the code.
* If you do not have a copy, see:
*
* http://www.gnu.org/licenses/lgpl-2.1.txt
* http://www.cecill.info/licences/Licence_CeCILL-C_V1-en.txt
*
* Copyright for this code is held jointly by the Genomic platform
* of the Institut de Biologie de l'École normale supérieure and
* the individual authors. These should be listed in @author doc
* comments.
*
* For more information on the Eoulsan project and its aims,
* or to join the Eoulsan Google group, visit the home page
* at:
*
* http://outils.genomique.biologie.ens.fr/eoulsan
*
*/
package fr.ens.biologie.genomique.eoulsan.bio.alignmentsfilters;
import htsjdk.samtools.SAMRecord;
import java.util.ArrayList;
import java.util.List;
import fr.ens.biologie.genomique.eoulsan.EoulsanException;
/**
* This alignments filter keep alignments of a read according to the distance of
* the read from the reference sequence on the genome. This filter is useful to
* detect SNP and indel.
* @since 1.2
* @author Claire Wallon
*/
public class DistanceFromReferenceReadAlignmentsFilter
extends AbstractReadAlignmentsFilter {
public static final String FILTER_NAME = "distancefromreference";
private int distance = -1;
@Override
public String getName() {
return FILTER_NAME;
}
@Override
public String getDescription() {
return "After this filter, only the alignments which the distance from "
+ "the reference is lower than the given distance are kept.";
}
@Override
public void setParameter(final String key, final String value)
throws EoulsanException {
if (key == null || value == null) {
return;
}
if ("threshold".equals(key.trim())) {
try {
this.distance = Integer.parseInt(value.trim());
} catch (NumberFormatException e) {
return;
}
if (this.distance < 0) {
throw new EoulsanException("Invalid distance: " + this.distance);
}
} else {
throw new EoulsanException(
"Unknown parameter for " + getName() + " alignments filter: " + key);
}
}
@Override
public void init() {
if (this.distance < 0) {
throw new IllegalArgumentException(
"The distance from the reference is not set for "
+ getName() + " alignments filter.");
}
}
@Override
public void filterReadAlignments(final List<SAMRecord> records) {
if (records == null) {
return;
}
List<SAMRecord> recordsToKeep = new ArrayList<>();
// single-end mode
if (!records.get(0).getReadPairedFlag()) {
for (SAMRecord r : records) {
if (!r.getCigarString().contains("S")
&& !r.getCigarString().contains("H") && r.getAttribute("NM") != null
&& r.getIntegerAttribute("NM") <= this.distance) {
recordsToKeep.add(r);
}
}
}
// paired-end mode
else {
for (int counterRecord = 0; counterRecord < records.size()
- 1; counterRecord += 2) {
final SAMRecord r1 = records.get(counterRecord);
final SAMRecord r2 = records.get(counterRecord + 1);
if (!r1.getCigarString().contains("S")
&& !r1.getCigarString().contains("H")
&& r1.getAttribute("NM") != null
&& r1.getIntegerAttribute("NM") <= this.distance
&& !r2.getCigarString().contains("S")
&& !r2.getCigarString().contains("H")
&& r2.getAttribute("NM") != null
&& r2.getIntegerAttribute("NM") <= this.distance) {
recordsToKeep.add(r1);
recordsToKeep.add(r2);
}
}
}
records.clear();
records.addAll(recordsToKeep);
}
@Override
public String toString() {
return this.getClass().getSimpleName()
+ "{name=" + getName() + ", distance=" + this.distance + "}";
}
}