/* * Eoulsan development code * * This code may be freely distributed and modified under the * terms of the GNU Lesser General Public License version 2.1 or * later and CeCILL-C. This should be distributed with the code. * If you do not have a copy, see: * * http://www.gnu.org/licenses/lgpl-2.1.txt * http://www.cecill.info/licences/Licence_CeCILL-C_V1-en.txt * * Copyright for this code is held jointly by the Genomic platform * of the Institut de Biologie de l'École normale supérieure and * the individual authors. These should be listed in @author doc * comments. * * For more information on the Eoulsan project and its aims, * or to join the Eoulsan Google group, visit the home page * at: * * http://outils.genomique.biologie.ens.fr/eoulsan * */ package fr.ens.biologie.genomique.eoulsan.bio; import htsjdk.samtools.SAMRecord; import java.io.Serializable; import java.util.Comparator; /** * This class is a Comparator for SAM records. * @since 1.2 * @author Claire Wallon */ public class SAMComparator implements Comparator<SAMRecord>, Serializable { private static final long serialVersionUID = -3948514532737476599L; @Override public int compare(final SAMRecord r0, final SAMRecord r1) { if (r0 == null) { throw new NullPointerException("r0 argument is null in SAMComparator"); } if (r1 == null) { throw new NullPointerException("r1 argument is null in SAMComparator"); } int comp; // Compare the two read names comp = r0.getReadName().compareTo(r1.getReadName()); if (comp == 0) { // Compare the mapping quality scores comp = r0.getMappingQuality() - r1.getMappingQuality(); if (comp == 0) { // Compare the reference names (chromosomes) comp = r0.getReferenceName().compareTo(r1.getReferenceName()); if (comp == 0) { // paired-end mode if (r0.getReadPairedFlag()) { // the two alignments to compare are a pair if (r0.getAlignmentStart() == r1.getMateAlignmentStart() && r1.getAlignmentStart() == r0.getMateAlignmentStart()) { comp = r0.getFlags() - r1.getFlags(); } // the two alignments to compare are part of different pairs else { if (r0.getFirstOfPairFlag() && r1.getFirstOfPairFlag()) { comp = r0.getAlignmentStart() - r1.getAlignmentStart(); if (comp == 0) { comp = r0.getMateAlignmentStart() - r1.getMateAlignmentStart(); } } else if (!r0.getFirstOfPairFlag() && !r1.getFirstOfPairFlag()) { comp = r0.getMateAlignmentStart() - r1.getMateAlignmentStart(); if (comp == 0) { comp = r0.getAlignmentStart() - r1.getAlignmentStart(); } } else if (r0.getFirstOfPairFlag() && !r1.getFirstOfPairFlag()) { comp = r0.getAlignmentStart() - r1.getMateAlignmentStart(); if (comp == 0) { comp = r0.getMateAlignmentStart() - r1.getAlignmentStart(); } } else { comp = r0.getMateAlignmentStart() - r1.getAlignmentStart(); if (comp == 0) { comp = r0.getAlignmentStart() - r1.getMateAlignmentStart(); } } if (comp == 0) { // Compare the CIGAR code comp = r0.getCigarString().compareTo(r1.getCigarString()); } } } // single-end mode else { // Compare the 1-based leftmost mapping position comp = r0.getAlignmentStart() - r1.getAlignmentStart(); if (comp == 0) { // Compare the end position of the alignment comp = r0.getAlignmentEnd() - r1.getAlignmentEnd(); if (comp == 0) { // Compare the CIGAR code comp = r0.getCigarString().compareTo(r1.getCigarString()); } } } } } } return comp; } }