/* * The MIT License * * Copyright (c) 2009 The Broad Institute * * Permission is hereby granted, free of charge, to any person obtaining a copy * of this software and associated documentation files (the "Software"), to deal * in the Software without restriction, including without limitation the rights * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell * copies of the Software, and to permit persons to whom the Software is * furnished to do so, subject to the following conditions: * * The above copyright notice and this permission notice shall be included in * all copies or substantial portions of the Software. * * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN * THE SOFTWARE. */ package picard.sam; import htsjdk.samtools.SAMFileHeader; import htsjdk.samtools.SAMFileReader; import htsjdk.samtools.SAMRecord; import htsjdk.samtools.SAMTextHeaderCodec; import htsjdk.samtools.util.AsciiWriter; import htsjdk.samtools.util.IOUtil; import picard.PicardException; import picard.cmdline.CommandLineProgram; import picard.cmdline.CommandLineProgramProperties; import picard.cmdline.Option; import picard.cmdline.StandardOptionDefinitions; import picard.cmdline.programgroups.SamOrBam; import java.io.File; import java.io.IOException; import java.io.PrintStream; /** * Very simple command that just reads a SAM or BAM file and writes out the header * and each records to standard out. * * @author tfennell@broad.mit.edu */ @CommandLineProgramProperties( usage = "Prints a SAM or BAM file to the screen.", usageShort = "Prints a SAM or BAM file to the screen", programGroup = SamOrBam.class ) public class ViewSam extends CommandLineProgram { public static enum AlignmentStatus { Aligned, Unaligned, All } public static enum PfStatus { PF, NonPF, All } @Option(shortName=StandardOptionDefinitions.INPUT_SHORT_NAME, doc="The SAM or BAM file to view.") public File INPUT; @Option(doc="Print out all reads, just the aligned reads or just the unaligned reads.") public AlignmentStatus ALIGNMENT_STATUS = AlignmentStatus.All; @Option(doc="Print out all reads, just the PF reads or just the non-PF reads.") public PfStatus PF_STATUS = PfStatus.All; public static void main(final String[] args) { new ViewSam().instanceMain(args); } @Override protected int doWork() { return writeSamText(System.out); } /** * This is factored out of doWork only for unit testing. */ int writeSamText(PrintStream printStream) { try { IOUtil.assertFileIsReadable(INPUT); final SAMFileReader in = new SAMFileReader(INPUT); final AsciiWriter writer = new AsciiWriter(printStream); final SAMFileHeader header = in.getFileHeader(); if (header.getTextHeader() != null) { writer.write(header.getTextHeader()); } else { // Headers that are too large are not retained as text, so need to regenerate text new SAMTextHeaderCodec().encode(writer, header, true); } for (final SAMRecord rec : in) { if (printStream.checkError()) { return 1; } if (this.ALIGNMENT_STATUS == AlignmentStatus.Aligned && rec.getReadUnmappedFlag()) continue; if (this.ALIGNMENT_STATUS == AlignmentStatus.Unaligned && !rec.getReadUnmappedFlag()) continue; if (this.PF_STATUS == PfStatus.PF && rec.getReadFailsVendorQualityCheckFlag()) continue; if (this.PF_STATUS == PfStatus.NonPF && !rec.getReadFailsVendorQualityCheckFlag()) continue; writer.write(rec.getSAMString()); } writer.flush(); if (printStream.checkError()) { return 1; } return 0; } catch (IOException e) { throw new PicardException("Exception writing SAM text", e); } } }