/* * The MIT License * * Copyright (c) 2014 The Broad Institute * * Permission is hereby granted, free of charge, to any person obtaining a copy * of this software and associated documentation files (the "Software"), to deal * in the Software without restriction, including without limitation the rights * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell * copies of the Software, and to permit persons to whom the Software is * furnished to do so, subject to the following conditions: * * The above copyright notice and this permission notice shall be included in * all copies or substantial portions of the Software. * * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN * THE SOFTWARE. */ package picard.illumina; import picard.illumina.parser.Tile; import picard.illumina.parser.TileTemplateRead; import htsjdk.samtools.metrics.MetricBase; import java.lang.String;import java.util.ArrayList; import java.util.Collection; /** * Metrics for Illumina Basecalling that stores median phasing and prephasing percentages on a per-template-read, per-lane basis. * For each lane/template read # (i.e. FIRST, SECOND) combination we will store the median values of both the phasing and prephasing * values for every tile in that lane/template read pair. * * @author jgentry */ public class IlluminaPhasingMetrics extends MetricBase { public long LANE; public String TYPE_NAME; public double PHASING_APPLIED; public double PREPHASING_APPLIED; /** Calculate the median phasing & prephasing values for a lane's tiles and create the appropriate IlluminaPhasingMetrics for them */ public static Collection<IlluminaPhasingMetrics> getPhasingMetricsForTiles(final long lane, final Collection<Tile> tilesForLane) { final LanePhasingMetricsCollector lanePhasingMetricsCollector = new LanePhasingMetricsCollector(tilesForLane); final Collection<IlluminaPhasingMetrics> phasingMetrics = new ArrayList<IlluminaPhasingMetrics>(); for (final TileTemplateRead tileTemplateRead : lanePhasingMetricsCollector.getMedianPhasingMap().keySet()) { final IlluminaPhasingMetrics phasingMetric = new IlluminaPhasingMetrics(); phasingMetric.LANE = lane; phasingMetric.TYPE_NAME = tileTemplateRead.toString(); phasingMetric.PHASING_APPLIED = lanePhasingMetricsCollector.getMedianPhasingMap().get(tileTemplateRead); phasingMetric.PREPHASING_APPLIED = lanePhasingMetricsCollector.getMedianPrePhasingMap().get(tileTemplateRead); phasingMetrics.add(phasingMetric); } return phasingMetrics; } /** This property is not exposed in a field to avoid complications with MetricBase's dependency on reflection. */ public static String getExtension() { return "illumina_phasing_metrics"; } }