/* * The MIT License * * Copyright (c) 2009 The Broad Institute * * Permission is hereby granted, free of charge, to any person obtaining a copy * of this software and associated documentation files (the "Software"), to deal * in the Software without restriction, including without limitation the rights * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell * copies of the Software, and to permit persons to whom the Software is * furnished to do so, subject to the following conditions: * * The above copyright notice and this permission notice shall be included in * all copies or substantial portions of the Software. * * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN * THE SOFTWARE. */ package picard.sam; import htsjdk.samtools.BamFileIoUtils; import htsjdk.samtools.SAMFileHeader; import htsjdk.samtools.SAMFileReader; import htsjdk.samtools.SAMFileWriter; import htsjdk.samtools.SAMFileWriterFactory; import htsjdk.samtools.SAMRecord; import htsjdk.samtools.ValidationStringency; import htsjdk.samtools.util.IOUtil; import htsjdk.samtools.util.Log; import htsjdk.samtools.util.ProgressLogger; import picard.PicardException; import picard.cmdline.CommandLineProgram; import picard.cmdline.CommandLineProgramProperties; import picard.cmdline.Option; import picard.cmdline.StandardOptionDefinitions; import picard.cmdline.programgroups.SamOrBam; import java.io.File; /** * @author alecw@broadinstitute.org */ @CommandLineProgramProperties( usage = "Replace the SAMFileHeader in a SAM file with the given header. " + "Validation is minimal. It is up to the user to ensure that all the elements referred to in the SAMRecords " + "are present in the new header. Sort order of the two input files must be the same.", usageShort = "Replace the SAMFileHeader in a SAM file with the given header", programGroup = SamOrBam.class ) public class ReplaceSamHeader extends CommandLineProgram { @Option(doc="SAM file from which SAMRecords will be read.", shortName = StandardOptionDefinitions.INPUT_SHORT_NAME) public File INPUT; @Option(doc="SAM file from which SAMFileHeader will be read.") public File HEADER; @Option(doc="SAMFileHeader from HEADER file will be written to this file, followed by SAMRecords from INPUT file", shortName = StandardOptionDefinitions.OUTPUT_SHORT_NAME) public File OUTPUT; public static void main(final String[] argv) { new ReplaceSamHeader().instanceMainWithExit(argv); } /** * Do the work after command line has been parsed. * RuntimeException may be thrown by this method, and are reported appropriately. * * @return program exit status. */ @Override protected int doWork() { IOUtil.assertFileIsReadable(INPUT); IOUtil.assertFileIsReadable(HEADER); IOUtil.assertFileIsWritable(OUTPUT); final SAMFileReader headerReader = new SAMFileReader(HEADER); final SAMFileHeader replacementHeader = headerReader.getFileHeader(); headerReader.close(); final ValidationStringency originalStringency = SAMFileReader.getDefaultValidationStringency(); SAMFileReader.setDefaultValidationStringency(ValidationStringency.SILENT); try { if (BamFileIoUtils.isBamFile(INPUT)) { blockCopyReheader(replacementHeader); } else { standardReheader(replacementHeader); } } finally { SAMFileReader.setDefaultValidationStringency(originalStringency); } return 0; } private void standardReheader(final SAMFileHeader replacementHeader) { final SAMFileReader recordReader = new SAMFileReader(INPUT); if (replacementHeader.getSortOrder() != recordReader.getFileHeader().getSortOrder()) { throw new PicardException("Sort orders of INPUT (" + recordReader.getFileHeader().getSortOrder().name() + ") and HEADER (" + replacementHeader.getSortOrder().name() + ") do not agree."); } final SAMFileWriter writer = new SAMFileWriterFactory().makeSAMOrBAMWriter(replacementHeader, true, OUTPUT); final ProgressLogger progress = new ProgressLogger(Log.getInstance(ReplaceSamHeader.class)); for (final SAMRecord rec : recordReader) { rec.setHeader(replacementHeader); writer.addAlignment(rec); progress.record(rec); } writer.close(); recordReader.close(); } private void blockCopyReheader(final SAMFileHeader replacementHeader) { BamFileIoUtils.reheaderBamFile(replacementHeader, INPUT, OUTPUT, CREATE_MD5_FILE, CREATE_INDEX); } }