/* * The MIT License * * Copyright (c) 2009 The Broad Institute * * Permission is hereby granted, free of charge, to any person obtaining a copy * of this software and associated documentation files (the "Software"), to deal * in the Software without restriction, including without limitation the rights * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell * copies of the Software, and to permit persons to whom the Software is * furnished to do so, subject to the following conditions: * * The above copyright notice and this permission notice shall be included in * all copies or substantial portions of the Software. * * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN * THE SOFTWARE. */ package htsjdk.samtools.reference; import org.testng.Assert; import org.testng.annotations.DataProvider; import org.testng.annotations.Test; import java.io.BufferedWriter; import java.io.File; import java.io.FileWriter; import java.util.Random; /** * Tests for the reading of reference sequences in various formats. * * @author Tim Fennell */ public class ReferenceSequenceTests { private static final byte[] BASES = "acgtACGTN".getBytes(); private final Random random = new Random(); @Test(dataProvider="fastaTestParameters") public void testSingleShortSequence(int chroms, int basesPerChrom) throws Exception { File f = makeRandomReference(chroms, basesPerChrom); ReferenceSequenceFile ref = ReferenceSequenceFileFactory.getReferenceSequenceFile(f); for (int i=1; i<=chroms; ++i) { ReferenceSequence seq = ref.nextSequence(); Assert.assertNotNull(seq); Assert.assertEquals(seq.length(), basesPerChrom); Assert.assertEquals(seq.getName(), "chr" + i); Assert.assertEquals(seq.getContigIndex(), i-1); } Assert.assertNull(ref.nextSequence()); } @DataProvider Object[][] fastaTestParameters() { return new Object[][] { new Object[] { 1, 60}, new Object[] { 2, 60}, new Object[] {10, 60}, new Object[] { 1, 1000}, new Object[] { 2, 1000}, new Object[] {10, 1000}, new Object[] { 1, 250000}, new Object[] { 2, 250000}, new Object[] {10, 250000} }; } /** Utility method to write a random reference sequence of specified length. */ private File makeRandomReference(int chroms, int basesPerChrom) throws Exception { File file = File.createTempFile("reference.", ".fasta"); file.deleteOnExit(); BufferedWriter out = new BufferedWriter(new FileWriter(file)); for (int i=1; i<=chroms; ++i) { out.write("> chr" + i); out.newLine(); for (int j=1; j<=basesPerChrom; ++j) { out.write(BASES[random.nextInt(BASES.length)]); if (j % 80 == 0 || j == basesPerChrom) out.newLine(); } } out.flush(); out.close(); return file; } }