/* * The MIT License * * Copyright (c) 2009 The Broad Institute * * Permission is hereby granted, free of charge, to any person obtaining a copy * of this software and associated documentation files (the "Software"), to deal * in the Software without restriction, including without limitation the rights * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell * copies of the Software, and to permit persons to whom the Software is * furnished to do so, subject to the following conditions: * * The above copyright notice and this permission notice shall be included in * all copies or substantial portions of the Software. * * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN * THE SOFTWARE. */ package picard.sam; import htsjdk.samtools.SAMFileHeader; import htsjdk.samtools.SAMFileReader; import htsjdk.samtools.SAMFileWriter; import htsjdk.samtools.SAMFileWriterFactory; import htsjdk.samtools.SAMRecord; import htsjdk.samtools.util.IOUtil; import htsjdk.samtools.util.Log; import htsjdk.samtools.util.ProgressLogger; import picard.cmdline.CommandLineProgram; import picard.cmdline.CommandLineProgramProperties; import picard.cmdline.Option; import picard.cmdline.StandardOptionDefinitions; import picard.cmdline.programgroups.SamOrBam; import java.io.File; /** * @author alecw@broadinstitute.org */ @CommandLineProgramProperties( usage = "Sorts the input SAM or BAM.\n" + "Input and output formats are determined by file extension.", usageShort = "Sorts a SAM or BAM file", programGroup = SamOrBam.class ) public class SortSam extends CommandLineProgram { @Option(doc="The BAM or SAM file to sort.", shortName= StandardOptionDefinitions.INPUT_SHORT_NAME) public File INPUT; @Option(doc="The sorted BAM or SAM output file. ", shortName=StandardOptionDefinitions.OUTPUT_SHORT_NAME) public File OUTPUT; @Option(shortName=StandardOptionDefinitions.SORT_ORDER_SHORT_NAME, doc="Sort order of output file") public SAMFileHeader.SortOrder SORT_ORDER; private final Log log = Log.getInstance(SortSam.class); public static void main(final String[] argv) { new SortSam().instanceMainWithExit(argv); } protected int doWork() { IOUtil.assertFileIsReadable(INPUT); IOUtil.assertFileIsWritable(OUTPUT); final SAMFileReader reader = new SAMFileReader(IOUtil.openFileForReading(INPUT)); reader.getFileHeader().setSortOrder(SORT_ORDER); final SAMFileWriter writer = new SAMFileWriterFactory().makeSAMOrBAMWriter(reader.getFileHeader(), false, OUTPUT); writer.setProgressLogger( new ProgressLogger(log, (int) 1e7, "Wrote", "records from a sorting collection")); final ProgressLogger progress = new ProgressLogger(log, (int) 1e7, "Read"); for (final SAMRecord rec: reader) { writer.addAlignment(rec); progress.record(rec); } log.info("Finished reading inputs, merging and writing to output now."); reader.close(); writer.close(); return 0; } }