package ij; import java.awt.*; import java.awt.image.*; import java.net.URL; import java.util.*; import ij.process.*; import ij.io.*; import ij.gui.*; import ij.measure.*; import ij.plugin.filter.Analyzer; import ij.util.Tools; import ij.macro.Interpreter; import ij.plugin.frame.ContrastAdjuster; import ij.plugin.frame.Recorder; import ij.plugin.Converter; import ij.plugin.Duplicator; import ij.plugin.RectToolOptions; /** An ImagePlus contain an ImageProcessor (2D image) or an ImageStack (3D, 4D or 5D image). It also includes metadata (spatial calibration and possibly the directory/file where it was read from). The ImageProcessor contains the pixel data (8-bit, 16-bit, float or RGB) of the 2D image and some basic methods to manipulate it. An ImageStack is essentually a list ImageProcessors of same type and size. @see ij.process.ImageProcessor @see ij.ImageStack @see ij.gui.ImageWindow @see ij.gui.ImageCanvas */ public class ImagePlus implements ImageObserver, Measurements, Cloneable { /** 8-bit grayscale (unsigned)*/ public static final int GRAY8 = 0; /** 16-bit grayscale (unsigned) */ public static final int GRAY16 = 1; /** 32-bit floating-point grayscale */ public static final int GRAY32 = 2; /** 8-bit indexed color */ public static final int COLOR_256 = 3; /** 32-bit RGB color */ public static final int COLOR_RGB = 4; /** True if any changes have been made to this image. */ public boolean changes; protected Image img; protected ImageProcessor ip; protected ImageWindow win; protected Roi roi; protected int currentSlice; protected static final int OPENED=0, CLOSED=1, UPDATED=2; protected boolean compositeImage; protected int width; protected int height; protected boolean locked = false; protected int nChannels = 1; protected int nSlices = 1; protected int nFrames = 1; private ImageJ ij = IJ.getInstance(); private String title; private String url; private FileInfo fileInfo; private int imageType = GRAY8; private ImageStack stack; private static int currentID = -1; private int ID; private static Component comp; private boolean imageLoaded; private int imageUpdateY, imageUpdateW; private Properties properties; private long startTime; private Calibration calibration; private static Calibration globalCalibration; private boolean activated; private boolean ignoreFlush; private boolean errorLoadingImage; private static ImagePlus clipboard; private static Vector listeners = new Vector(); private boolean openAsHyperStack; private int[] position = {1,1,1}; private boolean noUpdateMode; private ImageCanvas flatteningCanvas; private Overlay overlay; private boolean hideOverlay; private static int default16bitDisplayRange; /** Constructs an uninitialized ImagePlus. */ public ImagePlus() { ID = --currentID; title="null"; } /** Constructs an ImagePlus from an Image or BufferedImage. The first argument will be used as the title of the window that displays the image. Throws an IllegalStateException if an error occurs while loading the image. */ public ImagePlus(String title, Image img) { this.title = title; ID = --currentID; if (img!=null) setImage(img); } /** Constructs an ImagePlus from an ImageProcessor. */ public ImagePlus(String title, ImageProcessor ip) { setProcessor(title, ip); ID = --currentID; } /** Constructs an ImagePlus from a TIFF, BMP, DICOM, FITS, PGM, GIF or JPRG specified by a path or from a TIFF, DICOM, GIF or JPEG specified by a URL. */ public ImagePlus(String pathOrURL) { Opener opener = new Opener(); ImagePlus imp = null; boolean isURL = pathOrURL.indexOf("://")>0; if (isURL) imp = opener.openURL(pathOrURL); else imp = opener.openImage(pathOrURL); if (imp!=null) { if (imp.getStackSize()>1) setStack(imp.getTitle(), imp.getStack()); else setProcessor(imp.getTitle(), imp.getProcessor()); setCalibration(imp.getCalibration()); properties = imp.getProperties(); setFileInfo(imp.getOriginalFileInfo()); setDimensions(imp.getNChannels(), imp.getNSlices(), imp.getNFrames()); if (isURL) this.url = pathOrURL; ID = --currentID; } } /** Constructs an ImagePlus from a stack. */ public ImagePlus(String title, ImageStack stack) { setStack(title, stack); ID = --currentID; } /** Locks the image so other threads can test to see if it is in use. Returns true if the image was successfully locked. Beeps, displays a message in the status bar, and returns false if the image is already locked. */ public synchronized boolean lock() { if (locked) { IJ.beep(); IJ.showStatus("\"" + title + "\" is locked"); //if (IJ.macroRunning()) { // IJ.error("Image is locked"); // Macro.abort(); //} return false; } else { locked = true; if (IJ.debugMode) IJ.log(title + ": lock"); return true; } } /** Similar to lock, but doesn't beep and display an error message if the attempt to lock the image fails. */ public synchronized boolean lockSilently() { if (locked) return false; else { locked = true; if (IJ.debugMode) IJ.log(title + ": lock silently"); return true; } } /** Unlocks the image. */ public synchronized void unlock() { locked = false; if (IJ.debugMode) IJ.log(title + ": unlock"); } private void waitForImage(Image img) { if (comp==null) { comp = IJ.getInstance(); if (comp==null) comp = new Canvas(); } imageLoaded = false; if (!comp.prepareImage(img, this)) { double progress; waitStart = System.currentTimeMillis(); while (!imageLoaded && !errorLoadingImage) { //IJ.showStatus(imageUpdateY+" "+imageUpdateW); IJ.wait(30); if (imageUpdateW>1) { progress = (double)imageUpdateY/imageUpdateW; if (!(progress<1.0)) { progress = 1.0 - (progress-1.0); if (progress<0.0) progress = 0.9; } showProgress(progress); } } showProgress(1.0); } } long waitStart; private void showProgress(double percent) { if ((System.currentTimeMillis()-waitStart)>500L) IJ.showProgress(percent); } /** Draws the image. If there is an ROI, its outline is also displayed. Does nothing if there is no window associated with this image (i.e. show() has not been called).*/ public void draw(){ if (win!=null) win.getCanvas().repaint(); } /** Draws image and roi outline using a clip rect. */ public void draw(int x, int y, int width, int height){ if (win!=null) { ImageCanvas ic = win.getCanvas(); double mag = ic.getMagnification(); x = ic.screenX(x); y = ic.screenY(y); width = (int)(width*mag); height = (int)(height*mag); ic.repaint(x, y, width, height); if (listeners.size()>0 && roi!=null && roi.getPasteMode()!=Roi.NOT_PASTING) notifyListeners(UPDATED); } } /** Updates this image from the pixel data in its associated ImageProcessor, then displays it. Does nothing if there is no window associated with this image (i.e. show() has not been called).*/ public void updateAndDraw() { if (ip!=null) { if (win!=null) { win.getCanvas().setImageUpdated(); if (listeners.size()>0) notifyListeners(UPDATED); } draw(); } } /** Updates this image from the pixel data in its associated ImageProcessor, then displays it. The CompositeImage class overrides this method to only update the current channel. */ public void updateChannelAndDraw() { updateAndDraw(); } /** Returns a reference to the current ImageProcessor. The CompositeImage class overrides this method so it returns the processor associated with the current channel. */ public ImageProcessor getChannelProcessor() { return getProcessor(); } /* The CompositeImage class overrides this method to return, as an array, copies of this image's channel LUTs. */ public LUT[] getLuts() { return null; //ImageProcessor ip = getProcessor(); //ColorModel cm = ip.getColorModel(); //if (cm instanceof IndexColorModel) { // LUT[] luts = new LUT[1]; // luts[0] = new LUT((IndexColorModel)cm, ip.getMin(), ip.getMax()); // return luts; //} else // return null; } /** Calls draw to draw the image and also repaints the image window to force the information displayed above the image (dimension, type, size) to be updated. */ public void repaintWindow() { if (win!=null) { draw(); win.repaint(); } } /** Calls updateAndDraw to update from the pixel data and draw the image, and also repaints the image window to force the information displayed above the image (dimension, type, size) to be updated. */ public void updateAndRepaintWindow() { if (win!=null) { updateAndDraw(); win.repaint(); } } /** ImageCanvas.paint() calls this method when the ImageProcessor has generated new image. */ public void updateImage() { if (ip!=null) img = ip.createImage(); } /** Closes the window, if any, that is displaying this image. */ public void hide() { if (win==null) { Interpreter.removeBatchModeImage(this); return; } boolean unlocked = lockSilently(); changes = false; win.close(); win = null; if (unlocked) unlock(); } /** Closes this image and sets the ImageProcessor to null. To avoid the "Save changes?" dialog, first set the public 'changes' variable to false. */ public void close() { ImageWindow win = getWindow(); if (win!=null) { //if (IJ.isWindows() && IJ.isJava14()) // changes = false; // avoid 'save changes?' dialog and potential Java 1.5 deadlocks win.close(); } else { if (WindowManager.getCurrentImage()==this) WindowManager.setTempCurrentImage(null); killRoi(); //save any ROI so it can be restored later Interpreter.removeBatchModeImage(this); } } /** Opens a window to display this image and clears the status bar. */ public void show() { show(""); } /** Opens a window to display this image and displays 'statusMessage' in the status bar. */ public void show(String statusMessage) { if (win!=null) return; if ((IJ.isMacro() && ij==null) || Interpreter.isBatchMode()) { if (isComposite()) ((CompositeImage)this).reset(); ImagePlus img = WindowManager.getCurrentImage(); if (img!=null) img.saveRoi(); WindowManager.setTempCurrentImage(this); Interpreter.addBatchModeImage(this); return; } if (Prefs.useInvertingLut && getBitDepth()==8 && ip!=null && !ip.isInvertedLut()&& !ip.isColorLut()) invertLookupTable(); img = getImage(); if ((img!=null) && (width>=0) && (height>=0)) { activated = false; int stackSize = getStackSize(); //if (compositeImage) stackSize /= nChannels; if (stackSize>1) win = new StackWindow(this); else win = new ImageWindow(this); if (roi!=null) roi.setImage(this); if (overlay!=null && getCanvas()!=null) getCanvas().setOverlay(overlay); draw(); IJ.showStatus(statusMessage); if (IJ.isMacro()) { // wait for window to be activated long start = System.currentTimeMillis(); while (!activated) { IJ.wait(5); if ((System.currentTimeMillis()-start)>2000) { WindowManager.setTempCurrentImage(this); break; // 2 second timeout } } } if (imageType==GRAY16 && default16bitDisplayRange!=0) { resetDisplayRange(); updateAndDraw(); } if (stackSize>1) { int c = getChannel(); int z = getSlice(); int t = getFrame(); if (c>1 || z>1 || t>1) setPosition(c, z, t); } notifyListeners(OPENED); } } void invertLookupTable() { int nImages = getStackSize(); ip.invertLut(); if (nImages==1) ip.invert(); else { ImageStack stack2 = getStack(); for (int i=1; i<=nImages; i++) stack2.getProcessor(i).invert(); stack2.setColorModel(ip.getColorModel()); } } /** Called by ImageWindow.windowActivated(). */ public void setActivated() { activated = true; } /** Returns this image as a AWT image. */ public Image getImage() { if (img==null && ip!=null) img = ip.createImage(); return img; } /** Returns this image as a BufferedImage. */ public BufferedImage getBufferedImage() { if (isComposite()) return (new ColorProcessor(getImage())).getBufferedImage(); else return ip.getBufferedImage(); } /** Returns this image's unique numeric ID. */ public int getID() { return ID; } /** Replaces the image, if any, with the one specified. Throws an IllegalStateException if an error occurs while loading the image. */ public void setImage(Image img) { if (img instanceof BufferedImage) { BufferedImage bi = (BufferedImage)img; if (bi.getType()==BufferedImage.TYPE_USHORT_GRAY) { setProcessor(null, new ShortProcessor(bi)); return; } else if (bi.getType()==BufferedImage.TYPE_BYTE_GRAY) { setProcessor(null, new ByteProcessor(bi)); return; } } roi = null; errorLoadingImage = false; waitForImage(img); if (errorLoadingImage) throw new IllegalStateException ("Error loading image"); this.img = img; int newWidth = img.getWidth(ij); int newHeight = img.getHeight(ij); boolean dimensionsChanged = newWidth!=width || newHeight!=height; width = newWidth; height = newHeight; ip = null; stack = null; LookUpTable lut = new LookUpTable(img); int type; if (lut.getMapSize() > 0) { if (lut.isGrayscale()) type = GRAY8; else type = COLOR_256; } else type = COLOR_RGB; setType(type); setupProcessor(); this.img = ip.createImage(); if (win!=null) { if (dimensionsChanged) win = new ImageWindow(this); else repaintWindow(); } } /** Replaces this image with the specified ImagePlus. May not work as expected if 'imp' is a CompositeImage and this image is not. */ public void setImage(ImagePlus imp) { if (imp.getWindow()!=null) imp = imp.duplicate(); ImageStack stack2 = imp.getStack(); if (imp.isHyperStack()) setOpenAsHyperStack(true); setStack(stack2, imp.getNChannels(), imp.getNSlices(), imp.getNFrames()); } /** Replaces the ImageProcessor with the one specified and updates the display. */ public void setProcessor(ImageProcessor ip) { setProcessor(null, ip); } /** Replaces the ImageProcessor with the one specified and updates the display. Set 'title' to null to leave the image title unchanged. */ public void setProcessor(String title, ImageProcessor ip) { if (ip==null || ip.getPixels()==null) throw new IllegalArgumentException("ip null or ip.getPixels() null"); int stackSize = getStackSize(); if (stackSize>1 && (ip.getWidth()!=width || ip.getHeight()!=height)) throw new IllegalArgumentException("ip wrong size"); if (stackSize<=1) { stack = null; setCurrentSlice(1); } setProcessor2(title, ip, null); } void setProcessor2(String title, ImageProcessor ip, ImageStack newStack) { if (title!=null) setTitle(title); this.ip = ip; if (ij!=null) ip.setProgressBar(ij.getProgressBar()); int stackSize = 1; if (stack!=null) { stackSize = stack.getSize(); if (currentSlice>stackSize) setCurrentSlice(stackSize); } img = null; boolean dimensionsChanged = width>0 && height>0 && (width!=ip.getWidth() || height!=ip.getHeight()); if (dimensionsChanged) roi = null; int type; if (ip instanceof ByteProcessor) type = GRAY8; else if (ip instanceof ColorProcessor) type = COLOR_RGB; else if (ip instanceof ShortProcessor) type = GRAY16; else type = GRAY32; if (width==0) imageType = type; else setType(type); width = ip.getWidth(); height = ip.getHeight(); if (win!=null) { if (dimensionsChanged && stackSize==1) win.updateImage(this); else if (newStack==null) repaintWindow(); draw(); } } /** Replaces the image with the specified stack and updates the display. */ public void setStack(ImageStack stack) { setStack(null, stack); } /** Replaces the image with the specified stack and updates the display. Set 'title' to null to leave the title unchanged. */ public void setStack(String title, ImageStack newStack) { int newStackSize = newStack.getSize(); if (newStackSize==0) throw new IllegalArgumentException("Stack is empty"); if (!newStack.isVirtual()) { Object[] arrays = newStack.getImageArray(); if (arrays==null || (arrays.length>0&&arrays[0]==null)) throw new IllegalArgumentException("Stack pixel array null"); } boolean sliderChange = false; if (win!=null && (win instanceof StackWindow)) { int nScrollbars = ((StackWindow)win).getNScrollbars(); if (nScrollbars>0 && newStackSize==1) sliderChange = true; else if (nScrollbars==0 && newStackSize>1) sliderChange = true; } if (currentSlice<1) setCurrentSlice(1); boolean resetCurrentSlice = currentSlice>newStackSize; if (resetCurrentSlice) setCurrentSlice(newStackSize); ImageProcessor ip = newStack.getProcessor(currentSlice); boolean dimensionsChanged = width>0 && height>0 && (width!=ip.getWidth()||height!=ip.getHeight()); this.stack = newStack; setProcessor2(title, ip, newStack); if (win==null) { if (resetCurrentSlice) setSlice(currentSlice); return; } boolean invalidDimensions = isDisplayedHyperStack() && !((StackWindow)win).validDimensions(); if (newStackSize>1 && !(win instanceof StackWindow)) { if (isDisplayedHyperStack()) setOpenAsHyperStack(true); win = new StackWindow(this, getCanvas()); // replaces this window setPosition(1, 1, 1); } else if (newStackSize>1 && invalidDimensions) { if (isDisplayedHyperStack()) setOpenAsHyperStack(true); win = new StackWindow(this); // replaces this window setPosition(1, 1, 1); } else if (dimensionsChanged || sliderChange) win.updateImage(this); else repaintWindow(); if (resetCurrentSlice) setSlice(currentSlice); } public void setStack(ImageStack stack, int nChannels, int nSlices, int nFrames) { if (nChannels*nSlices*nFrames!=stack.getSize()) throw new IllegalArgumentException("channels*slices*frames!=stackSize"); this.nChannels = nChannels; this.nSlices = nSlices; this.nFrames = nFrames; if (isComposite()) ((CompositeImage)this).setChannelsUpdated(); setStack(null, stack); } /** Saves this image's FileInfo so it can be later retieved using getOriginalFileInfo(). */ public void setFileInfo(FileInfo fi) { if (fi!=null) fi.pixels = null; fileInfo = fi; } /** Returns the ImageWindow that is being used to display this image. Returns null if show() has not be called or the ImageWindow has been closed. */ public ImageWindow getWindow() { return win; } /** Returns true if this image is currently being displayed in a window. */ public boolean isVisible() { return win!=null && win.isVisible(); } /** This method should only be called from an ImageWindow. */ public void setWindow(ImageWindow win) { this.win = win; if (roi!=null) roi.setImage(this); // update roi's 'ic' field } /** Returns the ImageCanvas being used to display this image, or null. */ public ImageCanvas getCanvas() { return win!=null?win.getCanvas():flatteningCanvas; } /** Sets current foreground color. */ public void setColor(Color c) { if (ip!=null) ip.setColor(c); } void setupProcessor() { if (imageType==COLOR_RGB) { if (ip == null || ip instanceof ByteProcessor) ip = new ColorProcessor(getImage()); } else if (ip==null || (ip instanceof ColorProcessor)) ip = new ByteProcessor(getImage()); if (roi!=null && roi.isArea()) ip.setRoi(roi.getBounds()); else ip.resetRoi(); } public boolean isProcessor() { return ip!=null; } /** Returns a reference to the current ImageProcessor. If there is no ImageProcessor, it creates one. Returns null if this ImagePlus contains no ImageProcessor and no AWT Image. Sets the line width to the current line width and sets the calibration table if the image is density calibrated. */ public ImageProcessor getProcessor() { if (ip==null && img==null) return null; setupProcessor(); if (!compositeImage) ip.setLineWidth(Line.getWidth()); if (ij!=null) ip.setProgressBar(ij.getProgressBar()); Calibration cal = getCalibration(); if (cal.calibrated()) ip.setCalibrationTable(cal.getCTable()); else ip.setCalibrationTable(null); if (Recorder.record) { Recorder recorder = Recorder.getInstance(); if (recorder!=null) recorder.imageUpdated(this); } return ip; } /** Frees RAM by setting the snapshot (undo) buffer in the current ImageProcessor to null. */ public void trimProcessor() { ImageProcessor ip2 = ip; if (!locked && ip2!=null) { if (IJ.debugMode) IJ.log(title + ": trimProcessor"); Roi roi2 = getRoi(); if (roi2!=null && roi2.getPasteMode()!=Roi.NOT_PASTING) roi2.endPaste(); ip2.setSnapshotPixels(null); } } /** For images with irregular ROIs, returns a byte mask, otherwise, returns null. Mask pixels have a non-zero value. */ public ImageProcessor getMask() { if (roi==null) { if (ip!=null) ip.resetRoi(); return null; } ImageProcessor mask = roi.getMask(); if (mask==null) return null; if (ip!=null && roi!=null) { ip.setMask(mask); ip.setRoi(roi.getBounds()); } return mask; } /** Returns an ImageStatistics object generated using the standard measurement options (area, mean, mode, min and max). This plugin demonstrates how get the area, mean and max of the current image or selection: <pre> public class Get_Statistics implements PlugIn { public void run(String arg) { ImagePlus imp = IJ.getImage(); ImageStatistics stats = imp.getStatistics(); IJ.log("Area: "+stats.area); IJ.log("Mean: "+stats.mean); IJ.log("Max: "+stats.max); } } </pre> @see ij.process.ImageStatistics @see ij.process.ImageStatistics#getStatistics */ public ImageStatistics getStatistics() { return getStatistics(AREA+MEAN+MODE+MIN_MAX); } /** Returns an ImageStatistics object generated using the specified measurement options. This plugin demonstrates how get the area and centroid of the current selection: <pre> public class Get_Statistics implements PlugIn, Measurements { public void run(String arg) { ImagePlus imp = IJ.getImage(); ImageStatistics stats = imp.getStatistics(MEDIAN+CENTROID); IJ.log("Median: "+stats.median); IJ.log("xCentroid: "+stats.xCentroid); IJ.log("yCentroid: "+stats.yCentroid); } } </pre> @see ij.process.ImageStatistics @see ij.measure.Measurements */ public ImageStatistics getStatistics(int mOptions) { return getStatistics(mOptions, 256, 0.0, 0.0); } /** Returns an ImageStatistics object generated using the specified measurement options and histogram bin count. Note: except for float images, the number of bins is currently fixed at 256. */ public ImageStatistics getStatistics(int mOptions, int nBins) { return getStatistics(mOptions, nBins, 0.0, 0.0); } /** Returns an ImageStatistics object generated using the specified measurement options, histogram bin count and histogram range. Note: for 8-bit and RGB images, the number of bins is fixed at 256 and the histogram range is always 0-255. */ public ImageStatistics getStatistics(int mOptions, int nBins, double histMin, double histMax) { setupProcessor(); if (roi!=null && roi.isArea()) ip.setRoi(roi); else ip.resetRoi(); ip.setHistogramSize(nBins); Calibration cal = getCalibration(); if (getType()==GRAY16&& !(histMin==0.0&&histMax==0.0)) {histMin=cal.getRawValue(histMin); histMax=cal.getRawValue(histMax);} ip.setHistogramRange(histMin, histMax); ImageStatistics stats = ImageStatistics.getStatistics(ip, mOptions, cal); ip.setHistogramSize(256); ip.setHistogramRange(0.0, 0.0); return stats; } /** Returns the image name. */ public String getTitle() { if (title==null) return ""; else return title; } /** Returns a shortened version of image name that does not include spaces or a file name extension. */ public String getShortTitle() { String title = getTitle(); int index = title.indexOf(' '); if (index>-1) title = title.substring(0, index); index = title.lastIndexOf('.'); if (index>0) title = title.substring(0, index); return title; } /** Sets the image name. */ public void setTitle(String title) { if (title==null) return; if (win!=null) { if (ij!=null) Menus.updateWindowMenuItem(this.title, title); String virtual = stack!=null && stack.isVirtual()?" (V)":""; String global = getGlobalCalibration()!=null?" (G)":""; String scale = ""; double magnification = win.getCanvas().getMagnification(); if (magnification!=1.0) { double percent = magnification*100.0; int digits = percent>100.0||percent==(int)percent?0:1; scale = " (" + IJ.d2s(percent,digits) + "%)"; } win.setTitle(title+virtual+global+scale); } this.title = title; } public int getWidth() { return width; } public int getHeight() { return height; } /** If this is a stack, returns the number of slices, else returns 1. */ public int getStackSize() { if (stack==null) return 1; else { int slices = stack.getSize(); //if (compositeImage) slices /= nChannels; if (slices<=0) slices = 1; return slices; } } /** If this is a stack, returns the actual number of images in the stack, else returns 1. */ public int getImageStackSize() { if (stack==null) return 1; else { int slices = stack.getSize(); if (slices==0) slices = 1; return slices; } } /** Sets the 3rd, 4th and 5th dimensions, where <code>nChannels</code>*<code>nSlices</code>*<code>nFrames</code> must be equal to the stack size. */ public void setDimensions(int nChannels, int nSlices, int nFrames) { //IJ.log("setDimensions: "+nChannels+" "+nSlices+" "+nFrames+" "+getImageStackSize()); if (nChannels*nSlices*nFrames!=getImageStackSize() && ip!=null) { //throw new IllegalArgumentException("channels*slices*frames!=stackSize"); nChannels = 1; nSlices = getImageStackSize(); nFrames = 1; if (isDisplayedHyperStack()) { setOpenAsHyperStack(false); new StackWindow(this); setSlice(1); } } boolean updateWin = isDisplayedHyperStack() && (this.nChannels!=nChannels||this.nSlices!=nSlices||this.nFrames!=nFrames); this.nChannels = nChannels; this.nSlices = nSlices; this.nFrames = nFrames; if (updateWin) { if (nSlices!=getImageStackSize()) setOpenAsHyperStack(true); ip=null; img=null; setPositionWithoutUpdate(getChannel(), getSlice(), getFrame()); if (isComposite()) ((CompositeImage)this).reset(); new StackWindow(this); setPosition(getChannel(), getSlice(), getFrame()); } //IJ.log("setDimensions: "+ nChannels+" "+nSlices+" "+nFrames); } /** Returns 'true' if this image is a hyperstack. */ public boolean isHyperStack() { return isDisplayedHyperStack() || (openAsHyperStack&&getNDimensions()>3); } /** Returns the number of dimensions (2, 3, 4 or 5). */ public int getNDimensions() { int dimensions = 2; int[] dim = getDimensions(); if (dim[2]>1) dimensions++; if (dim[3]>1) dimensions++; if (dim[4]>1) dimensions++; return dimensions; } /** Returns 'true' if this is a hyperstack currently being displayed in a StackWindow. */ public boolean isDisplayedHyperStack() { return win!=null && win instanceof StackWindow && ((StackWindow)win).isHyperStack(); } /** Returns the number of channels. */ public int getNChannels() { verifyDimensions(); return nChannels; } /** Returns the image depth (number of z-slices). */ public int getNSlices() { //IJ.log("getNSlices: "+ nChannels+" "+nSlices+" "+nFrames); verifyDimensions(); return nSlices; } /** Returns the number of frames (time-points). */ public int getNFrames() { verifyDimensions(); return nFrames; } /** Returns the dimensions of this image (width, height, nChannels, nSlices, nFrames) as a 5 element int array. */ public int[] getDimensions() { verifyDimensions(); int[] d = new int[5]; d[0] = width; d[1] = height; d[2] = nChannels; d[3] = nSlices; d[4] = nFrames; return d; } void verifyDimensions() { int stackSize = getImageStackSize(); if (nSlices==1) { if (nChannels>1 && nFrames==1) nChannels = stackSize; else if (nFrames>1 && nChannels==1) nFrames = stackSize; } if (nChannels*nSlices*nFrames!=stackSize) { nSlices = stackSize; nChannels = 1; nFrames = 1; } } /** Returns the current image type (ImagePlus.GRAY8, ImagePlus.GRAY16, ImagePlus.GRAY32, ImagePlus.COLOR_256 or ImagePlus.COLOR_RGB). @see #getBitDepth */ public int getType() { return imageType; } /** Returns the bit depth, 8, 16, 24 (RGB) or 32. RGB images actually use 32 bits per pixel. */ public int getBitDepth() { int bitDepth = 0; switch (imageType) { case GRAY8: case COLOR_256: bitDepth=8; break; case GRAY16: bitDepth=16; break; case GRAY32: bitDepth=32; break; case COLOR_RGB: bitDepth=24; break; } return bitDepth; } /** Returns the number of bytes per pixel. */ public int getBytesPerPixel() { switch (imageType) { case GRAY16: return 2; case GRAY32: case COLOR_RGB: return 4; default: return 1; } } protected void setType(int type) { if ((type<0) || (type>COLOR_RGB)) return; int previousType = imageType; imageType = type; if (imageType!=previousType) { if (win!=null) Menus.updateMenus(); getLocalCalibration().setImage(this); } } /** Adds a key-value pair to this image's properties. The key is removed from the properties table if value is null. */ public void setProperty(String key, Object value) { if (properties==null) properties = new Properties(); if (value==null) properties.remove(key); else properties.put(key, value); } /** Returns the property associated with 'key'. May return null. */ public Object getProperty(String key) { if (properties==null) return null; else return properties.get(key); } /** Returns this image's Properties. May return null. */ public Properties getProperties() { return properties; } /** Creates a LookUpTable object that corresponds to this image. */ public LookUpTable createLut() { ImageProcessor ip2 = getProcessor(); if (ip2!=null) return new LookUpTable(ip2.getColorModel()); else return new LookUpTable(LookUpTable.createGrayscaleColorModel(false)); } /** Returns true is this image uses an inverting LUT that displays zero as white and 255 as black. */ public boolean isInvertedLut() { if (ip==null) { if (img==null) return false; setupProcessor(); } return ip.isInvertedLut(); } private int[] pvalue = new int[4]; /** Returns the pixel value at (x,y) as a 4 element array. Grayscale values are retuned in the first element. RGB values are returned in the first 3 elements. For indexed color images, the RGB values are returned in the first 3 three elements and the index (0-255) is returned in the last. */ public int[] getPixel(int x, int y) { pvalue[0]=pvalue[1]=pvalue[2]=pvalue[3]=0; switch (imageType) { case GRAY8: case COLOR_256: int index; if (ip!=null) index = ip.getPixel(x, y); else { byte[] pixels8; if (img==null) return pvalue; PixelGrabber pg = new PixelGrabber(img,x,y,1,1,false); try {pg.grabPixels();} catch (InterruptedException e){return pvalue;}; pixels8 = (byte[])(pg.getPixels()); index = pixels8!=null?pixels8[0]&0xff:0; } if (imageType!=COLOR_256) { pvalue[0] = index; return pvalue; } pvalue[3] = index; // fall through to get rgb values case COLOR_RGB: int c = 0; if (imageType==COLOR_RGB && ip!=null) c = ip.getPixel(x, y); else { int[] pixels32 = new int[1]; if (img==null) return pvalue; PixelGrabber pg = new PixelGrabber(img, x, y, 1, 1, pixels32, 0, width); try {pg.grabPixels();} catch (InterruptedException e) {return pvalue;}; c = pixels32[0]; } int r = (c&0xff0000)>>16; int g = (c&0xff00)>>8; int b = c&0xff; pvalue[0] = r; pvalue[1] = g; pvalue[2] = b; break; case GRAY16: case GRAY32: if (ip!=null) pvalue[0] = ip.getPixel(x, y); break; } return pvalue; } /** Returns an empty image stack that has the same width, height and color table as this image. */ public ImageStack createEmptyStack() { ColorModel cm; if (ip!=null) cm = ip.getColorModel(); else cm = createLut().getColorModel(); return new ImageStack(width, height, cm); } /** Returns the image stack. The stack may have only one slice. After adding or removing slices, call <code>setStack()</code> to update the image and the window that is displaying it. @see #setStack */ public ImageStack getStack() { ImageStack s; if (stack==null) { s = createEmptyStack(); ImageProcessor ip2 = getProcessor(); if (ip2==null) return s; String info = (String)getProperty("Info"); String label = info!=null?getTitle()+"\n"+info:null; s.addSlice(label, ip2); s.update(ip2); } else { s = stack; if (ip!=null) { Calibration cal = getCalibration(); if (cal.calibrated()) ip.setCalibrationTable(cal.getCTable()); else ip.setCalibrationTable(null); } s.update(ip); } if (roi!=null) s.setRoi(roi.getBounds()); else s.setRoi(null); return s; } /** Returns the base image stack. */ public ImageStack getImageStack() { if (stack==null) return getStack(); else { stack.update(ip); return stack; } } /** Returns the current stack slice number or 1 if this is a single image. */ public int getCurrentSlice() { if (currentSlice<1) setCurrentSlice(1); if (currentSlice>getStackSize()) setCurrentSlice(getStackSize()); return currentSlice; } final void setCurrentSlice(int slice) { currentSlice = slice; int stackSize = getStackSize(); if (nChannels==stackSize) updatePosition(currentSlice, 1, 1); if (nSlices==stackSize) updatePosition(1, currentSlice, 1); if (nFrames==stackSize) updatePosition(1, 1, currentSlice); } public int getChannel() { return position[0]; } public int getSlice() { return position[1]; } public int getFrame() { return position[2]; } public void killStack() { stack = null; trimProcessor(); } /** Sets the current hyperstack position and updates the display, where 'channel', 'slice' and 'frame' are one-based indexes. */ public void setPosition(int channel, int slice, int frame) { //IJ.log("setPosition: "+channel+" "+slice+" "+frame+" "+noUpdateMode); verifyDimensions(); if (channel<1) channel = 1; if (channel>nChannels) channel = nChannels; if (slice<1) slice = 1; if (slice>nSlices) slice = nSlices; if (frame<1) frame = 1; if (frame>nFrames) frame = nFrames; if (isDisplayedHyperStack()) ((StackWindow)win).setPosition(channel, slice, frame); else { setSlice((frame-1)*nChannels*nSlices + (slice-1)*nChannels + channel); updatePosition(channel, slice, frame); } } /** Sets the current hyperstack position without updating the display, where 'channel', 'slice' and 'frame' are one-based indexes. */ public void setPositionWithoutUpdate(int channel, int slice, int frame) { noUpdateMode = true; setPosition(channel, slice, frame); noUpdateMode = false; } /** Returns that stack index (one-based) corresponding to the specified position. */ public int getStackIndex(int channel, int slice, int frame) { if (channel<1) channel = 1; if (channel>nChannels) channel = nChannels; if (slice<1) slice = 1; if (slice>nSlices) slice = nSlices; if (frame<1) frame = 1; if (frame>nFrames) frame = nFrames; return (frame-1)*nChannels*nSlices + (slice-1)*nChannels + channel; } /* Hack needed to make the HyperStackReducer work. */ public void resetStack() { if (currentSlice==1 && stack!=null && stack.getSize()>0) { ColorModel cm = ip.getColorModel(); double min = ip.getMin(); double max = ip.getMax(); ip = stack.getProcessor(1); ip.setColorModel(cm); ip.setMinAndMax(min, max); } } /** Set the current hyperstack position based on the stack index 'n' (one-based). */ public void setPosition(int n) { int[] pos = convertIndexToPosition(n); setPosition(pos[0], pos[1], pos[2]); } /** Converts the stack index 'n' (one-based) into a hyperstack position (channel, slice, frame). */ public int[] convertIndexToPosition(int n) { if (n<1 || n>getStackSize()) throw new IllegalArgumentException("n out of range: "+n); int[] position = new int[3]; int[] dim = getDimensions(); position[0] = ((n-1)%dim[2])+1; position[1] = (((n-1)/dim[2])%dim[3])+1; position[2] = (((n-1)/(dim[2]*dim[3]))%dim[4])+1; return position; } /** Displays the specified stack image, where 1<=n<=stackSize. Does nothing if this image is not a stack. */ public synchronized void setSlice(int n) { if (stack==null || (n==currentSlice&&ip!=null)) { if (!noUpdateMode) updateAndRepaintWindow(); return; } if (n>=1 && n<=stack.getSize()) { Roi roi = getRoi(); if (roi!=null) roi.endPaste(); if (isProcessor()) stack.setPixels(ip.getPixels(),currentSlice); ip = getProcessor(); setCurrentSlice(n); Object pixels = stack.getPixels(currentSlice); if (ip!=null && pixels!=null) { ip.setSnapshotPixels(null); ip.setPixels(pixels); } else ip = stack.getProcessor(n); if (win!=null && win instanceof StackWindow) ((StackWindow)win).updateSliceSelector(); //if (IJ.altKeyDown() && !IJ.isMacro()) { // if (imageType==GRAY16 || imageType==GRAY32) { // ip.resetMinAndMax(); // IJ.showStatus(n+": min="+ip.getMin()+", max="+ip.getMax()); // } // ContrastAdjuster.update(); //} if (imageType==COLOR_RGB) ContrastAdjuster.update(); if (!noUpdateMode) updateAndRepaintWindow(); else img = null; } } /** Displays the specified stack image (1<=n<=stackSize) without updating the display. */ public void setSliceWithoutUpdate(int n) { noUpdateMode = true; setSlice(n); noUpdateMode = false; } /** Returns the current selection, or null if there is no selection. */ public Roi getRoi() { return roi; } /** Assigns the specified ROI to this image and displays it. Any existing ROI is deleted if <code>roi</code> is null or its width or height is zero. */ public void setRoi(Roi newRoi) { setRoi(newRoi, true); } /** Assigns 'newRoi' to this image and displays it if 'updateDisplay' is true. */ public void setRoi(Roi newRoi, boolean updateDisplay) { if (newRoi==null) {killRoi(); return;} if (newRoi.isVisible()) { newRoi = (Roi)newRoi.clone(); if (newRoi==null) {killRoi(); return;} } Rectangle bounds = newRoi.getBounds(); if (bounds.width==0 && bounds.height==0 && !(newRoi.getType()==Roi.POINT||newRoi.getType()==Roi.LINE)) {killRoi(); return;} roi = newRoi; if (ip!=null) { ip.setMask(null); if (roi.isArea()) ip.setRoi(bounds); else ip.resetRoi(); } roi.setImage(this); if (updateDisplay) draw(); } /** Creates a rectangular selection. */ public void setRoi(int x, int y, int width, int height) { setRoi(new Rectangle(x, y, width, height)); } /** Creates a rectangular selection. */ public void setRoi(Rectangle r) { setRoi(new Roi(r.x, r.y, r.width, r.height)); } /** Starts the process of creating a new selection, where sx and sy are the starting screen coordinates. The selection type is determined by which tool in the tool bar is active. The user interactively sets the selection size and shape. */ public void createNewRoi(int sx, int sy) { killRoi(); switch (Toolbar.getToolId()) { case Toolbar.RECTANGLE: int cornerDiameter = Toolbar.getRoundRectArcSize(); roi = new Roi(sx, sy, this, cornerDiameter); if (cornerDiameter>0) { roi.setStrokeColor(Toolbar.getForegroundColor()); roi.setStrokeWidth(RectToolOptions.getDefaultStrokeWidth()); } break; case Toolbar.OVAL: if (Toolbar.getOvalToolType()==Toolbar.ELLIPSE_ROI) roi = new EllipseRoi(sx, sy, this); else roi = new OvalRoi(sx, sy, this); break; case Toolbar.POLYGON: case Toolbar.POLYLINE: case Toolbar.ANGLE: roi = new PolygonRoi(sx, sy, this); break; case Toolbar.FREEROI: case Toolbar.FREELINE: roi = new FreehandRoi(sx, sy, this); break; case Toolbar.LINE: if ("arrow".equals(Toolbar.getToolName())) roi = new Arrow(sx, sy, this); else roi = new Line(sx, sy, this); break; case Toolbar.TEXT: roi = new TextRoi(sx, sy, this); break; case Toolbar.POINT: roi = new PointRoi(sx, sy, this); if (Prefs.pointAutoMeasure || (Prefs.pointAutoNextSlice&&!Prefs.pointAddToManager)) IJ.run("Measure"); if (Prefs.pointAddToManager) { IJ.run("Add to Manager "); ImageCanvas ic = getCanvas(); if (ic!=null && !ic.getShowAllROIs()) ic.setShowAllROIs(true); } if (Prefs.pointAutoNextSlice && getStackSize()>1) { IJ.run("Next Slice [>]"); killRoi(); } break; } } /** Deletes the current region of interest. Makes a copy of the current ROI so it can be recovered by the Edit/Selection/Restore Selection command. */ public void killRoi() { if (roi!=null) { saveRoi(); roi = null; if (ip!=null) ip.resetRoi(); draw(); } } public void saveRoi() { if (roi!=null) { roi.endPaste(); Rectangle r = roi.getBounds(); if ((r.width>0 || r.height>0)) { Roi.previousRoi = (Roi)roi.clone(); if (IJ.debugMode) IJ.log("saveRoi: "+roi); } } } public void restoreRoi() { if (Roi.previousRoi!=null) { Roi pRoi = Roi.previousRoi; Rectangle r = pRoi.getBounds(); if (r.width<=width || r.height<=height || isSmaller(pRoi)) { // will it (mostly) fit in this image? roi = (Roi)pRoi.clone(); roi.setImage(this); if (r.x>=width || r.y>=height || (r.x+r.width)<=0 || (r.y+r.height)<=0) // does it need to be moved? roi.setLocation((width-r.width)/2, (height-r.height)/2); else if (r.width==width && r.height==height) // is it the same size as the image roi.setLocation(0, 0); draw(); } } } boolean isSmaller(Roi r) { ImageProcessor mask = r.getMask(); if (mask==null) return false; mask.setThreshold(255, 255, ImageProcessor.NO_LUT_UPDATE); ImageStatistics stats = ImageStatistics.getStatistics(mask, MEAN+LIMIT, null); return stats.area<=width*height; } /** Implements the File/Revert command. */ public void revert() { if (getStackSize()>1 && getStack().isVirtual()) return; FileInfo fi = getOriginalFileInfo(); boolean isFileInfo = fi!=null && fi.fileFormat!=FileInfo.UNKNOWN; if (!(isFileInfo || url!=null)) return; if (ij!=null && changes && isFileInfo && !Interpreter.isBatchMode() && !IJ.isMacro() && !IJ.altKeyDown()) { if (!IJ.showMessageWithCancel("Revert?", "Revert to saved version of\n\""+getTitle()+"\"?")) return; } Roi saveRoi = null; if (roi!=null) { roi.endPaste(); saveRoi = (Roi)roi.clone(); } if (getStackSize()>1) { revertStack(fi); return; } trimProcessor(); if (isFileInfo && !(url!=null&&(fi.directory==null||fi.directory.equals("")))) new FileOpener(fi).revertToSaved(this); else if (url!=null) { IJ.showStatus("Loading: " + url); Opener opener = new Opener(); try { ImagePlus imp = opener.openURL(url); if (imp!=null) setProcessor(null, imp.getProcessor()); } catch (Exception e) {} if (getType()==COLOR_RGB && getTitle().endsWith(".jpg")) Opener.convertGrayJpegTo8Bits(this); } if (Prefs.useInvertingLut && getBitDepth()==8 && ip!=null && !ip.isInvertedLut()&& !ip.isColorLut()) invertLookupTable(); if (getProperty("FHT")!=null) { properties.remove("FHT"); if (getTitle().startsWith("FFT of ")) setTitle(getTitle().substring(7)); } ContrastAdjuster.update(); if (saveRoi!=null) setRoi(saveRoi); repaintWindow(); IJ.showStatus(""); changes = false; notifyListeners(UPDATED); } void revertStack(FileInfo fi) { String path = null; String url2 = null; if (url!=null && !url.equals("")) { path = url; url2 = url; } else if (fi!=null && !((fi.directory==null||fi.directory.equals("")))) { path = fi.directory+fi.fileName; } else if (fi!=null && fi.url!=null && !fi.url.equals("")) { path = fi.url; url2 = fi.url; } else return; //IJ.log("revert: "+path+" "+fi); IJ.showStatus("Loading: " + path); ImagePlus imp = IJ.openImage(path); if (imp!=null) { int n = imp.getStackSize(); int c = imp.getNChannels(); int z = imp.getNSlices(); int t = imp.getNFrames(); if (z==n || t==n || (c==getNChannels()&&z==getNSlices()&&t==getNFrames())) { setCalibration(imp.getCalibration()); setStack(imp.getStack(), c, z, t); } else { ImageWindow win = getWindow(); Point loc = null; if (win!=null) loc = win.getLocation(); changes = false; close(); FileInfo fi2 = imp.getOriginalFileInfo(); if (fi2!=null && (fi2.url==null || fi2.url.length()==0)) { fi2.url = url2; imp.setFileInfo(fi2); } ImageWindow.setNextLocation(loc); imp.show(); } } } /** Returns a FileInfo object containing information, including the pixel array, needed to save this image. Use getOriginalFileInfo() to get a copy of the FileInfo object used to open the image. @see ij.io.FileInfo @see #getOriginalFileInfo @see #setFileInfo */ public FileInfo getFileInfo() { FileInfo fi = new FileInfo(); fi.width = width; fi.height = height; fi.nImages = getStackSize(); if (compositeImage) fi.nImages = getImageStackSize(); fi.whiteIsZero = isInvertedLut(); fi.intelByteOrder = false; setupProcessor(); if (fi.nImages==1) fi.pixels = ip.getPixels(); else fi.pixels = stack.getImageArray(); Calibration cal = getCalibration(); if (cal.scaled()) { fi.pixelWidth = cal.pixelWidth; fi.pixelHeight = cal.pixelHeight; fi.unit = cal.getUnit(); } if (fi.nImages>1) fi.pixelDepth = cal.pixelDepth; fi.frameInterval = cal.frameInterval; if (cal.calibrated()) { fi.calibrationFunction = cal.getFunction(); fi.coefficients = cal.getCoefficients(); fi.valueUnit = cal.getValueUnit(); } switch (imageType) { case GRAY8: case COLOR_256: LookUpTable lut = createLut(); if (imageType==COLOR_256 || !lut.isGrayscale()) fi.fileType = FileInfo.COLOR8; else fi.fileType = FileInfo.GRAY8; fi.lutSize = lut.getMapSize(); fi.reds = lut.getReds(); fi.greens = lut.getGreens(); fi.blues = lut.getBlues(); break; case GRAY16: if (compositeImage && fi.nImages==3) fi.fileType = fi.RGB48; else fi.fileType = fi.GRAY16_UNSIGNED; break; case GRAY32: fi.fileType = fi.GRAY32_FLOAT; break; case COLOR_RGB: fi.fileType = fi.RGB; break; default: } return fi; } /** Returns the FileInfo object that was used to open this image. Returns null for images created using the File/New command. @see ij.io.FileInfo @see #getFileInfo */ public FileInfo getOriginalFileInfo() { if (fileInfo==null & url!=null) { fileInfo = new FileInfo(); fileInfo.width = width; fileInfo.height = height; fileInfo.url = url; fileInfo.directory = null; } return fileInfo; } /** Used by ImagePlus to monitor loading of images. */ public boolean imageUpdate(Image img, int flags, int x, int y, int w, int h) { imageUpdateY = y; imageUpdateW = w; if ((flags & ERROR) != 0) { errorLoadingImage = true; return false; } imageLoaded = (flags & (ALLBITS|FRAMEBITS|ABORT)) != 0; return !imageLoaded; } /** Sets the ImageProcessor, Roi, AWT Image and stack image arrays to null. Does nothing if the image is locked. */ public synchronized void flush() { notifyListeners(CLOSED); if (locked || ignoreFlush) return; ip = null; if (roi!=null) roi.setImage(null); roi = null; if (stack!=null) { Object[] arrays = stack.getImageArray(); if (arrays!=null) { for (int i=0; i<arrays.length; i++) arrays[i] = null; } } stack = null; img = null; win = null; if (roi!=null) roi.setImage(null); roi = null; properties = null; calibration = null; overlay = null; flatteningCanvas = null; } public void setIgnoreFlush(boolean ignoreFlush) { this.ignoreFlush = ignoreFlush; } /** Returns a copy (clone) of this ImagePlus. */ public ImagePlus duplicate() { return (new Duplicator()).run(this); } /** Returns a new ImagePlus with this image's attributes (e.g. spatial scale), but no image. */ public ImagePlus createImagePlus() { ImagePlus imp2 = new ImagePlus(); imp2.setType(getType()); imp2.setCalibration(getCalibration()); return imp2; } /** Returns a new hyperstack with this image's attributes (e.g., width, height, spatial scale), but no image data. */ public ImagePlus createHyperStack(String title, int channels, int slices, int frames, int bitDepth) { int size = channels*slices*frames; ImageStack stack2 = new ImageStack(width, height, size); // create empty stack ImageProcessor ip2 = null; switch (bitDepth) { case 8: ip2 = new ByteProcessor(width, height); break; case 16: ip2 = new ShortProcessor(width, height); break; case 24: ip2 = new ColorProcessor(width, height); break; case 32: ip2 = new FloatProcessor(width, height); break; default: throw new IllegalArgumentException("Invalid bit depth"); } stack2.setPixels(ip2.getPixels(), 1); // can't create ImagePlus will null 1st image ImagePlus imp2 = new ImagePlus(title, stack2); stack2.setPixels(null, 1); imp2.setDimensions(channels, slices, frames); imp2.setCalibration(getCalibration()); imp2.setOpenAsHyperStack(true); return imp2; } /** Copies the calibration of the specified image to this image. */ public void copyScale(ImagePlus imp) { if (imp!=null && globalCalibration==null) setCalibration(imp.getCalibration()); } /** Calls System.currentTimeMillis() to save the current time so it can be retrieved later using getStartTime() to calculate the elapsed time of an operation. */ public void startTiming() { startTime = System.currentTimeMillis(); } /** Returns the time in milliseconds when startTiming() was last called. */ public long getStartTime() { return startTime; } /** Returns this image's calibration. */ public Calibration getCalibration() { //IJ.log("getCalibration: "+globalCalibration+" "+calibration); if (globalCalibration!=null) { Calibration gc = globalCalibration.copy(); gc.setImage(this); return gc; } else { if (calibration==null) calibration = new Calibration(this); return calibration; } } /** Sets this image's calibration. */ public void setCalibration(Calibration cal) { //IJ.write("setCalibration: "+cal); if (cal==null) calibration = null; else { calibration = cal.copy(); calibration.setImage(this); } } /** Sets the system-wide calibration. */ public void setGlobalCalibration(Calibration global) { //IJ.log("setGlobalCalibration ("+getTitle()+"): "+global); if (global==null) globalCalibration = null; else globalCalibration = global.copy(); } /** Returns the system-wide calibration, or null. */ public Calibration getGlobalCalibration() { return globalCalibration; } /** Returns this image's local calibration, ignoring the "Global" calibration flag. */ public Calibration getLocalCalibration() { if (calibration==null) calibration = new Calibration(this); return calibration; } /** Displays the cursor coordinates and pixel value in the status bar. Called by ImageCanvas when the mouse moves. Can be overridden by ImagePlus subclasses. */ public void mouseMoved(int x, int y) { if (ij!=null) ij.showStatus(getLocationAsString(x,y) + getValueAsString(x,y)); savex=x; savey=y; } private int savex, savey; /** Redisplays the (x,y) coordinates and pixel value (which may have changed) in the status bar. Called by the Next Slice and Previous Slice commands to update the z-coordinate and pixel value. */ public void updateStatusbarValue() { IJ.showStatus(getLocationAsString(savex,savey) + getValueAsString(savex,savey)); } String getFFTLocation(int x, int y, Calibration cal) { double center = width/2.0; double r = Math.sqrt((x-center)*(x-center) + (y-center)*(y-center)); if (r<1.0) r = 1.0; double theta = Math.atan2(y-center, x-center); theta = theta*180.0/Math.PI; if (theta<0) theta = 360.0+theta; String s = "r="; if (cal.scaled()) s += IJ.d2s((width/r)*cal.pixelWidth,2) + " " + cal.getUnit() + "/c (" + IJ.d2s(r,0) + ")"; else s += IJ.d2s(width/r,2) + " p/c (" + IJ.d2s(r,0) + ")"; s += ", theta= " + IJ.d2s(theta,2) + IJ.degreeSymbol; return s; } /** Converts the current cursor location to a string. */ public String getLocationAsString(int x, int y) { Calibration cal = getCalibration(); if (getProperty("FHT")!=null) return getFFTLocation(x, height-y-1, cal); //y = Analyzer.updateY(y, height); if (!IJ.altKeyDown()) { String s = " x="+d2s(cal.getX(x)) + ", y=" + d2s(cal.getY(y,height)); if (getStackSize()>1) { int z = isDisplayedHyperStack()?getSlice()-1:getCurrentSlice()-1; s += ", z="+d2s(cal.getZ(z)); } return s; } else { String s = " x="+x+", y=" + y; if (getStackSize()>1) { int z = isDisplayedHyperStack()?getSlice()-1:getCurrentSlice()-1; s += ", z=" + z; } return s; } } private String d2s(double n) { return n==(int)n?Integer.toString((int)n):IJ.d2s(n); } private String getValueAsString(int x, int y) { if (win!=null && win instanceof PlotWindow) return ""; Calibration cal = getCalibration(); int[] v = getPixel(x, y); int type = getType(); switch (type) { case GRAY8: case GRAY16: case COLOR_256: if (type==COLOR_256) { if (cal.getCValue(v[3])==v[3]) // not calibrated return(", index=" + v[3] + ", value=" + v[0] + "," + v[1] + "," + v[2]); else v[0] = v[3]; } double cValue = cal.getCValue(v[0]); if (cValue==v[0]) return(", value=" + v[0]); else return(", value=" + IJ.d2s(cValue) + " ("+v[0]+")"); case GRAY32: return(", value=" + Float.intBitsToFloat(v[0])); case COLOR_RGB: return(", value=" + v[0] + "," + v[1] + "," + v[2]); default: return(""); } } /** Copies the contents of the current selection to the internal clipboard. Copies the entire image if there is no selection. Also clears the selection if <code>cut</code> is true. */ public void copy(boolean cut) { Roi roi = getRoi(); if (roi!=null && !roi.isArea()) { IJ.error("Cut/Copy", "The Cut and Copy commands require\n" +"an area selection, or no selection."); return; } boolean batchMode = Interpreter.isBatchMode(); String msg = (cut)?"Cutt":"Copy"; if (!batchMode) IJ.showStatus(msg+ "ing..."); ImageProcessor ip = getProcessor(); ImageProcessor ip2; Roi roi2 = null; ip2 = ip.crop(); if (roi!=null && roi.getType()!=Roi.RECTANGLE) { roi2 = (Roi)roi.clone(); Rectangle r = roi.getBounds(); if (r.x<0 || r.y<0 || r.x+r.width>width || r.y+r.height>height) { roi2 = new ShapeRoi(roi2); ShapeRoi image = new ShapeRoi(new Roi(0, 0, width, height)); roi2 = image.and((ShapeRoi)roi2); } } clipboard = new ImagePlus("Clipboard", ip2); if (roi2!=null) clipboard.setRoi(roi2); if (cut) { ip.snapshot(); ip.setColor(Toolbar.getBackgroundColor()); ip.fill(); if (roi!=null && roi.getType()!=Roi.RECTANGLE) { getMask(); ip.reset(ip.getMask()); } setColor(Toolbar.getForegroundColor()); Undo.setup(Undo.FILTER, this); updateAndDraw(); } int bytesPerPixel = 1; switch (clipboard.getType()) { case ImagePlus.GRAY16: bytesPerPixel = 2; break; case ImagePlus.GRAY32: case ImagePlus.COLOR_RGB: bytesPerPixel = 4; } //Roi roi3 = clipboard.getRoi(); //IJ.log("copy: "+clipboard +" "+ "roi3="+(roi3!=null?""+roi3:"")); if (!batchMode) { msg = (cut)?"Cut":"Copy"; IJ.showStatus(msg + ": " + (clipboard.getWidth()*clipboard.getHeight()*bytesPerPixel)/1024 + "k"); } } /** Inserts the contents of the internal clipboard into the active image. If there is a selection the same size as the image on the clipboard, the image is inserted into that selection, otherwise the selection is inserted into the center of the image.*/ public void paste() { if (clipboard==null) return; int cType = clipboard.getType(); int iType = getType(); int w = clipboard.getWidth(); int h = clipboard.getHeight(); Roi cRoi = clipboard.getRoi(); Rectangle r = null; Roi roi = getRoi(); if (roi!=null) r = roi.getBounds(); //if (w==width && h==height && (r==null||w!=r.width||h!=r.height)) { // setRoi(0, 0, width, height); // roi = getRoi(); // r = roi.getBounds(); //} if (r==null || (r!=null && (w!=r.width || h!=r.height))) { // create a new roi centered on visible part of image ImageCanvas ic = null; if (win!=null) ic = win.getCanvas(); Rectangle srcRect = ic!=null?ic.getSrcRect():new Rectangle(0,0,width, height); int xCenter = srcRect.x + srcRect.width/2; int yCenter = srcRect.y + srcRect.height/2; if (cRoi!=null && cRoi.getType()!=Roi.RECTANGLE) { cRoi.setImage(this); cRoi.setLocation(xCenter-w/2, yCenter-h/2); setRoi(cRoi); } else setRoi(xCenter-w/2, yCenter-h/2, w, h); roi = getRoi(); } if (IJ.isMacro()) { //non-interactive paste int pasteMode = Roi.getCurrentPasteMode(); boolean nonRect = roi.getType()!=Roi.RECTANGLE; ImageProcessor ip = getProcessor(); if (nonRect) ip.snapshot(); r = roi.getBounds(); ip.copyBits(clipboard.getProcessor(), r.x, r.y, pasteMode); if (nonRect) ip.reset(getMask()); updateAndDraw(); //killRoi(); } else if (roi!=null) { roi.startPaste(clipboard); Undo.setup(Undo.PASTE, this); } changes = true; } /** Returns the internal clipboard or null if the internal clipboard is empty. */ public static ImagePlus getClipboard() { return clipboard; } /** Clears the internal clipboard. */ public static void resetClipboard() { clipboard = null; } protected void notifyListeners(int id) { synchronized (listeners) { for (int i=0; i<listeners.size(); i++) { ImageListener listener = (ImageListener)listeners.elementAt(i); switch (id) { case OPENED: listener.imageOpened(this); break; case CLOSED: listener.imageClosed(this); break; case UPDATED: listener.imageUpdated(this); break; } } } } public static void addImageListener(ImageListener listener) { listeners.addElement(listener); } public static void removeImageListener(ImageListener listener) { listeners.removeElement(listener); } /** Returns 'true' if the image is locked. */ public boolean isLocked() { return locked; } public void setOpenAsHyperStack(boolean openAsHyperStack) { this.openAsHyperStack = openAsHyperStack; } public boolean getOpenAsHyperStack() { return openAsHyperStack; } /** Returns true if this is a CompositeImage. */ public boolean isComposite() { return compositeImage && nChannels>=1 && (this instanceof CompositeImage); } /** Sets the display range of the current channel. With non-composite images it is identical to ip.setMinAndMax(min, max). */ public void setDisplayRange(double min, double max) { if (ip!=null) ip.setMinAndMax(min, max); } public double getDisplayRangeMin() { return ip.getMin(); } public double getDisplayRangeMax() { return ip.getMax(); } /** Sets the display range of specified channels in an RGB image, where 4=red, 2=green, 1=blue, 6=red+green, etc. With non-RGB images, this method is identical to setDisplayRange(min, max). This method is used by the Image/Adjust/Color Balance tool . */ public void setDisplayRange(double min, double max, int channels) { if (ip instanceof ColorProcessor) ((ColorProcessor)ip).setMinAndMax(min, max, channels); else ip.setMinAndMax(min, max); } public void resetDisplayRange() { if (imageType==GRAY16 && default16bitDisplayRange>=8 && default16bitDisplayRange<=16 && !(getCalibration().isSigned16Bit())) { ip.setMinAndMax(0, Math.pow(2,default16bitDisplayRange)-1); } else ip.resetMinAndMax(); } /** Set the default 16-bit display range, where 'bitDepth' must be 0 (auto-scaling), 8 (0-255), 10 (0-1023), 12 (0-4095, 15 (0-32767) or 16 (0-65535). */ public static void setDefault16bitRange(int bitDepth) { if (!(bitDepth==8 || bitDepth==10 || bitDepth==12 || bitDepth==15 || bitDepth==16)) bitDepth = 0; default16bitDisplayRange = bitDepth; } /** Returns the default 16-bit display range, 0 (auto-scaling), 8, 10, 12, 15 or 16. */ public static int getDefault16bitRange() { return default16bitDisplayRange; } public void updatePosition(int c, int z, int t) { //IJ.log("updatePosition: "+c+", "+z+", "+t); position[0] = c; position[1] = z; position[2] = t; } /** Returns a "flattened" version of this image, in RGB format. */ public ImagePlus flatten() { ImagePlus imp2 = createImagePlus(); String title = "Flat_"+getTitle(); ImageCanvas ic2 = new ImageCanvas(imp2); imp2.flatteningCanvas = ic2; imp2.setRoi(getRoi()); ImageCanvas ic = getCanvas(); Overlay overlay2 = getOverlay(); ic2.setOverlay(overlay2); if (ic!=null) { ic2.setShowAllROIs(ic.getShowAllROIs()); //double mag = ic.getMagnification(); //if (mag<1.0) ic2.setMagnification(mag); } BufferedImage bi = new BufferedImage(width, height, BufferedImage.TYPE_INT_ARGB); Graphics g = bi.getGraphics(); g.drawImage(getImage(), 0, 0, null); ic2.paint(g); imp2.flatteningCanvas = null; if (Recorder.record) Recorder.recordCall("imp = IJ.getImage().flatten();"); return new ImagePlus(title, new ColorProcessor(bi)); } /** Installs a list of ROIs that will be drawn on this image as a non-destructive overlay. * @see ij.gui.Roi#setStrokeColor * @see ij.gui.Roi#setStrokeWidth * @see ij.gui.Roi#setFillColor * @see ij.gui.Roi#setLocation * @see ij.gui.Roi#setNonScalable */ public void setOverlay(Overlay overlay) { ImageCanvas ic = getCanvas(); if (ic!=null) { ic.setOverlay(overlay); overlay = null; } else this.overlay = overlay; setHideOverlay(false); } /** Creates an Overlay from the specified Shape, Color * and BasicStroke, and assigns it to this image. * @see #setOverlay(ij.gui.Overlay) * @see ij.gui.Roi#setStrokeColor * @see ij.gui.Roi#setStrokeWidth */ public void setOverlay(Shape shape, Color color, BasicStroke stroke) { if (shape==null) {setOverlay(null); return;} Roi roi = new ShapeRoi(shape); roi.setStrokeColor(color); roi.setStroke(stroke); setOverlay(new Overlay(roi)); } /** Creates an Overlay from the specified ROI, and assigns it to this image. * @see #setOverlay(ij.gui.Overlay) */ public void setOverlay(Roi roi, Color strokeColor, int strokeWidth, Color fillColor) { roi.setStrokeColor(strokeColor); roi.setStrokeWidth(strokeWidth); roi.setFillColor(fillColor); setOverlay(new Overlay(roi)); } /** Returns the current overly, or null if this image does not have an overlay. */ public Overlay getOverlay() { ImageCanvas ic = getCanvas(); if (ic!=null) return ic.getOverlay(); else return overlay; } public void setHideOverlay(boolean hide) { hideOverlay = hide; ImageCanvas ic = getCanvas(); if (ic!=null && ic.getOverlay()!=null) ic.repaint(); } public boolean getHideOverlay() { return hideOverlay; } /** Returns a shallow copy of this ImagePlus. */ public synchronized Object clone() { try { ImagePlus copy = (ImagePlus)super.clone(); copy.win = null; return copy; } catch (CloneNotSupportedException e) { return null; } } public String toString() { return "imp["+getTitle()+" "+width+"x"+height+"x"+getStackSize()+"]"; } }