/* * The MIT License * * Copyright (c) 2009 The Broad Institute * * Permission is hereby granted, free of charge, to any person obtaining a copy * of this software and associated documentation files (the "Software"), to deal * in the Software without restriction, including without limitation the rights * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell * copies of the Software, and to permit persons to whom the Software is * furnished to do so, subject to the following conditions: * * The above copyright notice and this permission notice shall be included in * all copies or substantial portions of the Software. * * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN * THE SOFTWARE. */ package picard.sam; import htsjdk.samtools.BamFileIoUtils; import htsjdk.samtools.SAMFileHeader; import htsjdk.samtools.SAMFileWriter; import htsjdk.samtools.SAMFileWriterFactory; import htsjdk.samtools.SAMRecord; import htsjdk.samtools.SamReader; import htsjdk.samtools.SamReaderFactory; import htsjdk.samtools.ValidationStringency; import htsjdk.samtools.util.CloserUtil; import htsjdk.samtools.util.IOUtil; import htsjdk.samtools.util.Log; import htsjdk.samtools.util.ProgressLogger; import picard.PicardException; import picard.cmdline.CommandLineProgram; import picard.cmdline.CommandLineProgramProperties; import picard.cmdline.Option; import picard.cmdline.StandardOptionDefinitions; import picard.cmdline.programgroups.SamOrBam; import java.io.File; /** * @author alecw@broadinstitute.org */ @CommandLineProgramProperties( usage = ReplaceSamHeader.USAGE_SUMMARY + ReplaceSamHeader.USAGE_DETAILS, usageShort = ReplaceSamHeader.USAGE_SUMMARY, programGroup = SamOrBam.class ) public class ReplaceSamHeader extends CommandLineProgram { static final String USAGE_SUMMARY = "Replaces the SAMFileHeader in a SAM or BAM file. "; static final String USAGE_DETAILS = "This tool makes it possible to replace the header of a SAM or BAM file with the header of another" + "file, or a header block that has been edited manually (in a stub SAM file). The sort order (@SO) of the two input files must " + "be the same.<br /><br />" + "Note that validation is minimal, so it is up to the user to ensure that all the elements referred to in the SAMRecords " + "are present in the new header. " + "<br />" + "<h4>Usage example:</h4>" + "<pre>" + "java -jar picard.jar ReplaceSamHeader \\<br />" + " I=input_1.bam \\<br />" + " HEADER=input_2.bam \\<br />" + " O=bam_with_new_head.bam" + "</pre>" + "<hr />"; @Option(doc = "SAM file from which SAMRecords will be read.", shortName = StandardOptionDefinitions.INPUT_SHORT_NAME) public File INPUT; @Option(doc = "SAM file from which SAMFileHeader will be read.") public File HEADER; @Option(doc = "SAMFileHeader from HEADER file will be written to this file, followed by SAMRecords from INPUT file", shortName = StandardOptionDefinitions.OUTPUT_SHORT_NAME) public File OUTPUT; public static void main(final String[] argv) { new ReplaceSamHeader().instanceMainWithExit(argv); } /** * Do the work after command line has been parsed. * RuntimeException may be thrown by this method, and are reported appropriately. * * @return program exit status. */ @Override protected int doWork() { IOUtil.assertFileIsReadable(INPUT); IOUtil.assertFileIsReadable(HEADER); IOUtil.assertFileIsWritable(OUTPUT); final SAMFileHeader replacementHeader = SamReaderFactory.makeDefault().referenceSequence(REFERENCE_SEQUENCE).getFileHeader(HEADER); if (BamFileIoUtils.isBamFile(INPUT)) { blockCopyReheader(replacementHeader); } else { standardReheader(replacementHeader); } return 0; } private void standardReheader(final SAMFileHeader replacementHeader) { final SamReader recordReader = SamReaderFactory.makeDefault().referenceSequence(REFERENCE_SEQUENCE).validationStringency(ValidationStringency.SILENT).open(INPUT); if (replacementHeader.getSortOrder() != recordReader.getFileHeader().getSortOrder()) { throw new PicardException("Sort orders of INPUT (" + recordReader.getFileHeader().getSortOrder().name() + ") and HEADER (" + replacementHeader.getSortOrder().name() + ") do not agree."); } final SAMFileWriter writer = new SAMFileWriterFactory().makeSAMOrBAMWriter(replacementHeader, true, OUTPUT); final ProgressLogger progress = new ProgressLogger(Log.getInstance(ReplaceSamHeader.class)); for (final SAMRecord rec : recordReader) { rec.setHeader(replacementHeader); writer.addAlignment(rec); progress.record(rec); } writer.close(); CloserUtil.close(recordReader); } private void blockCopyReheader(final SAMFileHeader replacementHeader) { BamFileIoUtils.reheaderBamFile(replacementHeader, INPUT, OUTPUT, CREATE_MD5_FILE, CREATE_INDEX); } }