/******************************************************************************* * GenPlay, Einstein Genome Analyzer * Copyright (C) 2009, 2014 Albert Einstein College of Medicine * * This program is free software: you can redistribute it and/or modify * it under the terms of the GNU General Public License as published by * the Free Software Foundation, either version 3 of the License, or * (at your option) any later version. * * This program is distributed in the hope that it will be useful, * but WITHOUT ANY WARRANTY; without even the implied warranty of * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the * GNU General Public License for more details. * * You should have received a copy of the GNU General Public License * along with this program. If not, see <http://www.gnu.org/licenses/>. * Authors: Julien Lajugie <julien.lajugie@einstein.yu.edu> * Nicolas Fourel <nicolas.fourel@einstein.yu.edu> * Eric Bouhassira <eric.bouhassira@einstein.yu.edu> * * Website: <http://genplay.einstein.yu.edu> ******************************************************************************/ package edu.yu.einstein.genplay.core.IO.utils; import edu.yu.einstein.genplay.core.IO.extractor.StrandedExtractor; import edu.yu.einstein.genplay.dataStructure.chromosome.Chromosome; import edu.yu.einstein.genplay.dataStructure.chromosomeWindow.SimpleChromosomeWindow; import edu.yu.einstein.genplay.dataStructure.enums.Strand; /** * Options for {@link StrandedExtractor}. * Allows user to choose which strand to extract and to define a fragment size * @author Julien Lajugie */ public class StrandedExtractorOptions { private final Strand selectedStrand; // strand to extract. Both if null private final int fragmentLength; // length of the fragments private final int readLength; // length of the reads /** * Creates an instance of {@link StrandedExtractorOptions} * @param strandToExtract strand to extract. Both strand will be extracted if this parameter is null * @param fragmentLength length of the fragments * @param readLength length of the reads */ public StrandedExtractorOptions(Strand strandToExtract, int fragmentLength, int readLength) { super(); selectedStrand = strandToExtract; this.fragmentLength = fragmentLength; this.readLength = readLength; } /** * Computes the new start and stop positions of a specified read after applying the read length options. * @param chromo chromosome of the read * @param start start position of the read * @param stop stop position of the read * @param strand strand of the read * @return a {@link SimpleChromosomeWindow} with the result start and stop positions */ public SimpleChromosomeWindow computeStartStop(Chromosome chromo, int start, int stop, Strand strand) { if ((strand == null) || (chromo == null) || (readLength == 0) || (fragmentLength == 0)) { // we return the value without modifying them return new SimpleChromosomeWindow(start, stop); } if (strand == Strand.THREE) { // case where the read is on the 3' strand // we want to make sure that the result positions are not smaller than 1 start = Math.max(1, (start + readLength) - fragmentLength); } // we want to make sure that the result positions are not greater than the chromosome length stop = Math.min(chromo.getLength(), start + fragmentLength); return new SimpleChromosomeWindow(start, stop); } /** * @return the fragment length value */ public int getFragmentLength() { return fragmentLength; } /** * @return the read length value */ public int getReadLength() { return readLength; } /** * @return the {@link Strand} selected to be extracted. Null means that both strands are selected to be extracted. */ public Strand getSelectedStrand() { return selectedStrand; } /** * @param strand a {@link Strand} * @return true if the specified strand is selected to be extracted. False otherwise. */ public boolean isSelected(Strand strand) { if (selectedStrand == null) { return true; } return selectedStrand == strand; } }