/* * The MIT License (MIT) * * Copyright (c) 2007-2015 Broad Institute * * Permission is hereby granted, free of charge, to any person obtaining a copy * of this software and associated documentation files (the "Software"), to deal * in the Software without restriction, including without limitation the rights * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell * copies of the Software, and to permit persons to whom the Software is * furnished to do so, subject to the following conditions: * * The above copyright notice and this permission notice shall be included in * all copies or substantial portions of the Software. * * * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN * THE SOFTWARE. */ package org.broad.igv.tools.converters; import org.broad.igv.feature.*; import org.broad.igv.feature.genome.Genome; import org.broad.igv.feature.tribble.GFFCodec; import org.broad.igv.util.ResourceLocator; import org.broad.igv.util.TestUtils; import htsjdk.tribble.Feature; import org.junit.Test; import java.io.BufferedReader; import java.io.File; import java.io.FileReader; import java.util.List; import static junit.framework.Assert.assertEquals; import static junit.framework.Assert.assertTrue; /** * @author Jim Robinson * @date 2/15/12 */ public class GFFtoBedTest { @Test public void testConvert() throws Exception { File inputFile = new File(TestUtils.DATA_DIR, "gff/gene.unsorted.gff3"); File outputFile = new File(TestUtils.DATA_DIR, "out/gene.bed"); // Convert GFFtoBed.convert(inputFile, outputFile); // Now compare features from both files, they should be identical BufferedReader gffReader = null; BufferedReader bedReader = null; try { Genome genome = null; // Not needed for this test gffReader = new BufferedReader(new FileReader(inputFile)); GFFParser parser = new GFFParser(); GFFCodec.Version version = inputFile.getPath().endsWith(".gff3") ? GFFCodec.Version.GFF3 : GFFCodec.Version.GFF2; GFFCodec codec = new GFFCodec(version, null); List<Feature> gffFeatures = parser.loadFeatures(gffReader, genome, codec); bedReader = new BufferedReader(new FileReader(outputFile)); FeatureParser bedParser = AbstractFeatureParser.getInstanceFor(new ResourceLocator(outputFile.getAbsolutePath()), null); List<Feature> bedFeatures = bedParser.loadFeatures(bedReader, genome); assertTrue(gffFeatures.size() > 0); assertEquals(gffFeatures.size(), bedFeatures.size()); for (int i = 0; i < gffFeatures.size(); i++) { BasicFeature gffFeature = (BasicFeature) gffFeatures.get(i); BasicFeature bedFeature = (BasicFeature) bedFeatures.get(i); compare(gffFeature, bedFeature); } } finally { gffReader.close(); } } private void compare(BasicFeature gffFeature, BasicFeature bedFeature) { assertEquals(gffFeature.getChr(), bedFeature.getChr()); assertEquals(gffFeature.getStart(), bedFeature.getStart()); assertEquals(gffFeature.getEnd(), bedFeature.getEnd()); assertEquals(gffFeature.getStrand(), bedFeature.getStrand()); assertEquals(gffFeature.getColor(), bedFeature.getColor()); //BED features don't have a type //assertEquals(gffFeature.getType(), bedFeature.getType()); assertEquals(gffFeature.getStart(), bedFeature.getThickStart()); assertEquals(gffFeature.getThickEnd(), bedFeature.getThickEnd()); if (!Float.isNaN(gffFeature.getScore())) { assertEquals(gffFeature.getScore(), bedFeature.getScore()); } assertEquals(gffFeature.getExonCount(), bedFeature.getExonCount()); List<Exon> gffExons = gffFeature.getExons(); List<Exon> bedExons = bedFeature.getExons(); // This is broken for sure, disable for now // for (int i = 0; i < gffExons.size(); i++) { // // Exon gffExon = gffExons.get(i); // Exon bedExon = bedExons.get(i); // assertEquals(gffExon.getCdStart(), bedExon.getCdStart()); // assertEquals(gffExon.getCodingLength(), bedExon.getCodingLength()); // assertEquals(gffExon.getCdEnd(), bedExon.getCdEnd()); // assertEquals(gffExon.getReadingShift(), bedExon.getReadingShift()); // } } }