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package org.broad.igv.feature.tribble;
import org.broad.igv.AbstractHeadlessTest;
import org.broad.igv.feature.BasicFeature;
import org.broad.igv.tools.IgvTools;
import org.broad.igv.util.TestUtils;
import htsjdk.tribble.AbstractFeatureReader;
import htsjdk.variant.vcf.VCFCodec;
import org.junit.Assert;
import org.junit.Test;
import java.util.Arrays;
import java.util.HashSet;
import java.util.Iterator;
import java.util.Set;
import static junit.framework.Assert.assertTrue;
import static org.junit.Assert.assertEquals;
/**
* @author jrobinso
* @date Aug 9, 2010
*/
public class TribbleIndexTest extends AbstractHeadlessTest {
/**
* chr2 1 200000000 LONG_FEATURE
* ...
* chr2 179098961 179380395 Hs.134602
* chr2 179209546 179287210 Hs.620337
* chr2 179266309 179266748 Hs.609465
* chr2 179296428 179300012 Hs.623987
* chr2 179302952 179303488 Hs.594545
*/
@Test
public void testLinearIndex() throws Exception {
String bedFile = TestUtils.DATA_DIR + "bed/Unigene.sample.bed";
String chr = "chr2";
int start = 179266309 - 1;
int end = 179303488 + 1;
int expectedCount = 6;
Set<String> expectedNames = new HashSet<String>(Arrays.asList("Hs.134602", "Hs.620337", "Hs.609465", "Hs.623987",
"Hs.594545", "LONG_FEATURE"));
// Interval index
TestUtils.createIndex(bedFile, IgvTools.LINEAR_INDEX, 500);
AbstractFeatureReader bfr = AbstractFeatureReader.getFeatureReader(bedFile, new IGVBEDCodec());
Iterator<BasicFeature> iter = bfr.query(chr, start, end);
int countInterval = 0;
while (iter.hasNext()) {
BasicFeature feature = iter.next();
Assert.assertTrue(feature.getEnd() >= start && feature.getStart() <= end);
Assert.assertTrue(expectedNames.contains(feature.getName()));
countInterval++;
}
assertEquals(expectedCount, countInterval);
}
@Test
/**
* Test interval tree index
* chr2 179098961 179380395 Hs.134602
* chr2 179209546 179287210 Hs.620337
* chr2 179266309 179266748 Hs.609465
* chr2 179296428 179300012 Hs.623987
* chr2 179302952 179303488 Hs.594545
*
*/
public void testIntervalTree() throws Exception {
//chr2:179,222,066-179,262,059<- CONTAINS TTN
String bedFile = TestUtils.DATA_DIR + "bed/Unigene.sample.bed";
String chr = "chr2";
int start = 179266309 - 1;
int end = 179303488 + 1;
int expectedCount = 6;
Set<String> expectedNames = new HashSet<String>(Arrays.asList("Hs.134602", "Hs.620337", "Hs.609465", "Hs.623987",
"Hs.594545", "LONG_FEATURE"));
// Interval index
TestUtils.createIndex(bedFile, IgvTools.INTERVAL_INDEX, 1);
AbstractFeatureReader bfr = AbstractFeatureReader.getFeatureReader(bedFile, new IGVBEDCodec());
Iterator<BasicFeature> iter = bfr.query(chr, start, end);
int countInterval = 0;
while (iter.hasNext()) {
BasicFeature feature = iter.next();
Assert.assertTrue(feature.getEnd() >= start && feature.getStart() <= end);
Assert.assertTrue(expectedNames.contains(feature.getName()));
countInterval++;
}
assertEquals(expectedCount, countInterval);
}
@Test
public void testReadSingleVCF() throws Exception {
String file = TestUtils.DATA_DIR + "vcf/indel_variants_onerow.vcf";
String chr = "chr9";
// Linear index
TestUtils.createIndex(file);
// First test query
AbstractFeatureReader bfr = AbstractFeatureReader.getFeatureReader(file, new VCFCodec());
Iterator<htsjdk.variant.variantcontext.VariantContext> iter = bfr.query(chr, 5073767 - 5, 5073767 + 5);
int count = 0;
while (iter.hasNext()) {
htsjdk.variant.variantcontext.VariantContext feat = iter.next();
assertEquals("chr9", feat.getChr());
assertEquals(feat.getStart(), 5073767);
assertTrue(feat.hasAttribute("MapQs"));
count++;
}
assertEquals(1, count);
// Test non-indexed access (iterator)
iter = bfr.iterator();
count = 0;
while (iter.hasNext()) {
htsjdk.variant.variantcontext.VariantContext feat = iter.next();
assertEquals("chr9", feat.getChr());
assertEquals(feat.getStart(), 5073767);
assertTrue(feat.hasAttribute("MapQs"));
count++;
}
assertEquals(1, count);
//Do similar as above, but have a different test file
file = TestUtils.DATA_DIR + "vcf/outputPileup.flt1.vcf";
chr = "1";
// Linear index
TestUtils.createIndex(file);
bfr = AbstractFeatureReader.getFeatureReader(file, new VCFCodec());
iter = bfr.query(chr, 984163 - 5, 984163 + 5);
count = 0;
while (iter.hasNext()) {
htsjdk.variant.variantcontext.VariantContext feat = iter.next();
assertEquals(chr, feat.getChr());
if (count == 0) {
assertEquals(984163, feat.getStart());
}
count++;
}
assertEquals(1, count);
}
}