/* The MIT License (MIT) Copyright (c) 2017 Pierre Lindenbaum Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ package com.github.lindenb.jvarkit.tools.vcfviewgui; import java.io.File; import java.io.FileOutputStream; import java.io.IOException; import java.io.InputStream; import java.net.URL; import java.util.Optional; import java.util.logging.Logger; import htsjdk.samtools.BAMIndex; import htsjdk.samtools.BamFileIoUtils; import htsjdk.samtools.SAMFileHeader; import htsjdk.samtools.SAMRecord; import htsjdk.samtools.SAMSequenceDictionary; import htsjdk.samtools.SamInputResource; import htsjdk.samtools.SamReader; import htsjdk.samtools.SamReaderFactory; import htsjdk.samtools.ValidationStringency; import htsjdk.samtools.util.CloseableIterator; import htsjdk.samtools.util.CloserUtil; import htsjdk.samtools.util.IOUtil; public class BamFile implements NgsFile<SAMFileHeader,SAMRecord>{ private static final Logger LOG= Logger.getLogger("BamFile"); private final SamReader samReader; private final String source; private final Optional<File> indexFile; private boolean delete_index_on_close=true; private BamFile(final String source, final SamReader samReader,final Optional<File> indexFile) throws IOException { this.samReader = samReader; this.source = source; this.indexFile = indexFile; if(!this.samReader.hasIndex()) { this.samReader.close(); throw new IOException("Bam without index "+source); } if(this.samReader.getFileHeader()==null) { this.samReader.close(); throw new IOException("Bam without header "+source); } if(this.samReader.getFileHeader().getSequenceDictionary()==null) { this.samReader.close(); throw new IOException("Bam without dictionary "+source); } } public static BamFile newInstance(final String s) throws IOException { return IOUtil.isUrl(s)? newInstance(new URL(s)): newInstance(new File(s)) ; } public static BamFile newInstance(final File f) throws IOException { IOUtil.assertFileIsReadable(f); final SamReaderFactory srf = SamReaderFactory.makeDefault(); srf.validationStringency(ValidationStringency.LENIENT); return new BamFile(f.getAbsolutePath(),srf.open(f),Optional.empty()); } public static BamFile newInstance(final URL url) throws IOException { final File baiFile=File.createTempFile("tmp.", BAMIndex.BAMIndexSuffix); Optional<File> savedBaiFile=Optional.empty(); baiFile.deleteOnExit(); for(int i=0;i< 2;++i) { final String bamurl=url.toExternalForm(); final String baiurl=(i==0? bamurl:bamurl.substring(0, bamurl.length()-BamFileIoUtils.BAM_FILE_EXTENSION.length()) )+ BAMIndex.BAMIndexSuffix; InputStream in=null; FileOutputStream out=null; try { LOG.info("trying "+baiurl); in = new URL(baiurl).openStream(); out = new FileOutputStream(baiFile); IOUtil.copyStream(in, out); out.flush(); out.close(); in.close(); savedBaiFile = Optional.of(baiFile); break; } catch (final IOException err) { baiFile.delete(); LOG.info("Cannot fetch "+baiurl+" : "+err.getMessage()); } finally { CloserUtil.close(out); CloserUtil.close(in); } } if(!savedBaiFile.isPresent()) { throw new IOException("cannot get a bam index file for "+url); } final SamReaderFactory srf = SamReaderFactory.makeDefault(); srf.validationStringency(ValidationStringency.LENIENT); final SamInputResource sir= SamInputResource.of(url); sir.index(savedBaiFile.get()); return new BamFile(url.toExternalForm(),srf.open(sir),savedBaiFile); } @Override public SAMFileHeader getHeader() { return samReader.getFileHeader(); } @Override public SAMSequenceDictionary getSequenceDictionary() { return getHeader().getSequenceDictionary(); } public CloseableIterator<SAMRecord> queryUnmapped() throws IOException { return samReader.queryUnmapped(); } @Override public CloseableIterator<SAMRecord> iterator() throws IOException { return samReader.iterator(); } @Override public CloseableIterator<SAMRecord> iterator(String contig, int start, int end) throws IOException { return samReader.query(contig,start,end,false); } @Override public void close() { if(this.indexFile.isPresent() && this.delete_index_on_close) { this.indexFile.get().delete(); } CloserUtil.close(this.samReader); } @Override public String getSource() { return this.source; } @Override public BamFile reOpen() throws IOException { final String url=this.getSource(); final SamReaderFactory srf = SamReaderFactory.makeDefault(); srf.validationStringency(ValidationStringency.LENIENT); final SamInputResource sir; if(IOUtil.isUrl(url)) { sir = SamInputResource.of(new URL(url)); if(!this.indexFile.isPresent()) throw new IOException("Boum"); sir.index(this.indexFile.get()); } else { sir = SamInputResource.of(new File(url)); } final BamFile bf = new BamFile(url,srf.open(sir),this.indexFile); bf.delete_index_on_close=false; return bf; } }