/* The MIT License (MIT) Copyright (c) 2014 Pierre Lindenbaum Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. History: * 2015 : moving to knime * 2014 : creation */ package com.github.lindenb.jvarkit.tools.misc; import java.io.File; import java.io.IOException; import java.util.List; import htsjdk.samtools.SAMSequenceDictionary; import htsjdk.samtools.util.CloseableIterator; import htsjdk.samtools.util.CloserUtil; import htsjdk.samtools.util.SortingCollection; import htsjdk.tribble.util.TabixUtils; import htsjdk.variant.variantcontext.VariantContext; import htsjdk.variant.variantcontext.writer.VariantContextWriter; import htsjdk.variant.variantcontext.writer.VariantContextWriterBuilder; import htsjdk.variant.variantcontext.writer.VariantContextWriterBuilder.OutputType; import htsjdk.variant.vcf.VCFHeader; import htsjdk.variant.vcf.VCFRecordCodec; import com.beust.jcommander.Parameter; import com.beust.jcommander.ParametersDelegate; import com.github.lindenb.jvarkit.util.jcommander.Launcher; import com.github.lindenb.jvarkit.util.jcommander.Program; import com.github.lindenb.jvarkit.util.log.Logger; import com.github.lindenb.jvarkit.util.picard.SAMSequenceDictionaryProgress; import com.github.lindenb.jvarkit.util.vcf.VcfIterator; /** BEGIN_DOC ## Example ```bash $ java -jar dist/vcfshuffle.jar in.vcf |\ java -jar dist/vcfindextabix.jar -s -o out.vcf.gz ``` END_DOC */ @Program(name="vcfindextabix",description="Index and sort a VCF on the fly with Tabix") public class VcfIndexTabix extends Launcher { private static final Logger LOG = Logger.build(VcfIndexTabix.class).make(); @Parameter(names={"-o","--output"},description="Output file. Optional . Default: stdout") private File outputFile = null; @Parameter(names={"-s","--sort"},description="sort VCF prior to saving") private boolean sort = false; @ParametersDelegate private WritingSortingCollection writingSortingCollection = new WritingSortingCollection(); private class SortingVCFWriter implements VariantContextWriter { VariantContextWriter delegate; SortingCollection<VariantContext> sorter=null; SortingVCFWriter(VariantContextWriter delegate) { this.delegate=delegate; } @Override public boolean checkError() { return delegate.checkError(); } @Override public void writeHeader(VCFHeader header) { this.delegate.writeHeader(header); this.sorter = SortingCollection.newInstance( VariantContext.class, new VCFRecordCodec(header), header.getVCFRecordComparator(), VcfIndexTabix.this.writingSortingCollection.getMaxRecordsInRam(), VcfIndexTabix.this.writingSortingCollection.getTmpDirectories() ); } @Override public void add(VariantContext vc) { this.sorter.add(vc); } @Override public void close() { CloseableIterator<VariantContext> iter=null; try { this.sorter.doneAdding(); this.sorter.setDestructiveIteration(true); iter= this.sorter.iterator(); while(iter.hasNext()) { this.delegate.add(iter.next()); } this.sorter.cleanup(); } catch(Exception err) { throw new RuntimeException(err); } finally { CloserUtil.close(iter); CloserUtil.close(this.delegate); } } } public VcfIndexTabix() { } private int doVcfToVcf(String inputName, final VcfIterator vcfIn,final File outFile) throws IOException { SortingVCFWriter sortingVCW=null; VariantContextWriterBuilder vcwb=new VariantContextWriterBuilder(); VariantContextWriter w=null; try { SAMSequenceDictionary dict = vcfIn.getHeader().getSequenceDictionary(); if(dict!=null) vcwb.setReferenceDictionary(dict); vcwb.setOutputFile(outFile); vcwb.setOutputFileType(OutputType.BLOCK_COMPRESSED_VCF); w = vcwb.build(); if(this.sort) { LOG.info("Creating a sorting writer"); sortingVCW= new SortingVCFWriter(w); w=sortingVCW; } w.writeHeader(vcfIn.getHeader()); SAMSequenceDictionaryProgress progress=new SAMSequenceDictionaryProgress(vcfIn.getHeader()); while(vcfIn.hasNext()) { w.add(progress.watch(vcfIn.next())); } progress.finish(); w.close(); w=null; return RETURN_OK; } catch (Exception e) { if(outFile.exists() && outFile.isFile()) { LOG.warn("Deleting "+outFile); outFile.delete(); File tbi = new File(outFile.getPath()+TabixUtils.STANDARD_INDEX_EXTENSION); if(tbi.exists() && tbi.isFile()) tbi.delete(); } LOG.error(e); return -1; } finally { CloserUtil.close(w); } } @Override public int doWork(List<String> args) { if(outputFile==null) { LOG.error(" output file undefined."); return -1; } if(!outputFile.getName().endsWith(".vcf.gz")) { LOG.error("output file should en with .vcf.gz but got "+outputFile); return 1; } VcfIterator iter = null; try { final String inputName=oneFileOrNull(args); iter = super.openVcfIterator(inputName); return doVcfToVcf(inputName==null?"STDIN":inputName, iter, outputFile); } catch (Exception e) { LOG.error(e); return -1; } finally { CloserUtil.close(iter); } } public static void main(String[] args) { new VcfIndexTabix().instanceMainWithExit(args); } }