/*
The MIT License (MIT)
Copyright (c) 2015 Pierre Lindenbaum
Permission is hereby granted, free of charge, to any person obtaining a copy
of this software and associated documentation files (the "Software"), to deal
in the Software without restriction, including without limitation the rights
to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
copies of the Software, and to permit persons to whom the Software is
furnished to do so, subject to the following conditions:
The above copyright notice and this permission notice shall be included in all
copies or substantial portions of the Software.
THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE
SOFTWARE.
History:
* 2014 creation
*/
package com.github.lindenb.jvarkit.tools.biostar;
import java.io.File;
import java.util.ArrayList;
import java.util.HashMap;
import java.util.List;
import java.util.Map;
import htsjdk.samtools.CigarOperator;
import htsjdk.samtools.SAMFileHeader;
import htsjdk.samtools.SAMFileHeader.SortOrder;
import htsjdk.samtools.Cigar;
import htsjdk.samtools.CigarElement;
import htsjdk.samtools.DefaultSAMRecordFactory;
import htsjdk.samtools.SAMFileWriter;
import htsjdk.samtools.SAMFileWriterFactory;
import htsjdk.samtools.SAMProgramRecord;
import htsjdk.samtools.SAMRecord;
import htsjdk.samtools.SAMSequenceDictionary;
import htsjdk.samtools.SAMSequenceRecord;
import htsjdk.samtools.util.CloserUtil;
import htsjdk.tribble.readers.LineIterator;
import com.beust.jcommander.Parameter;
import com.beust.jcommander.ParametersDelegate;
import com.github.lindenb.jvarkit.io.IOUtils;
import com.github.lindenb.jvarkit.util.jcommander.Launcher;
import com.github.lindenb.jvarkit.util.jcommander.Program;
import com.github.lindenb.jvarkit.util.log.Logger;
/**
BEGIN_DOC
## Example
```bash
$ curl -sL "https://raw.githubusercontent.com/suryasaha/Pred_cutoff/60a6f980c9940dfb6e381c5394918f27cb14564f/data/Xylella-RpoH.aln" |\
java -jar dist-1.128/biostar139647.jar
@HD VN:1.4 SO:unsorted
@SQ SN:chrUn LN:42
@PG ID:0 VN:3a0c4ccb05e7492382e00328ac60951f215d9400 CL:(empty) PN:Biostar139647
1 0 chrUn 1 60 42M * 0 0 CATACTTGGTCATCGGTCGTGTCCTTGAAAGTGACTTGTTAA *
2 0 chrUn 1 60 42M * 0 0 TCTCTGAACCCCCTTGAAACCCCTACACTCAGCCATATATGC *
3 0 chrUn 1 60 42M * 0 0 TACCTTCGGGTCCTTGAAAATAGCGTCGCCGTGCTTATCTGT *
4 0 chrUn 1 60 5M2D35M * 0 0 TTGACAGCCGCTTGAGCAGGCGTCGGTCATCCCCACATTC *
5 0 chrUn 1 60 18M1D9M1D13M * 0 0 ATGCCTGGGTGGCTTGAAAGCTGGCGGCTTGCCCACATAC *
6 0 chrUn 1 60 20M1D21M * 0 0 TCAGTTTTATCGCTTGATATTCACTGAGACTGGCCACACAT *
```
```
$ curl -sL "https://raw.github.com/biopython/biopython/master/Tests/Clustalw/opuntia.aln" 2> /dev/null | java -jar dist-1.128/biostar139647.jar
@HD VN:1.4 SO:unsorted
@SQ SN:chrUn LN:156
@PG ID:0 VN:3a0c4ccb05e7492382e00328ac60951f215d9400 CL:(empty) PN:Biostar139647
gi|6273285|gb|AF191659.1|AF191 0 chrUn 1 60 56M10D90M * 0 0 TATACATTAAAGAAGGGGGATGCGGATAAATGGAAAGGCGAAAGAAAGAATATATAATATATTTCA
AATTTCCTTATATACCCAAATATAAAAATATCTAATAAATTAGATGAATATCAAAGAATCCATTGATTTAGTGTACCAGA*
gi|6273284|gb|AF191658.1|AF191 0 chrUn 1 60 58M8D90M * 0 0 TATACATTAAAGAAGGGGGATGCGGATAAATGGAAAGGCGAAAGAAAGAATATATATAATATATTT
CAAATTTCCTTATATACCCAAATATAAAAATATCTAATAAATTAGATGAATATCAAAGAATCTATTGATTTAGTGTACCAGA *
gi|6273289|gb|AF191663.1|AF191 0 chrUn 1 60 60M6D90M * 0 0 TATACATTAAAGGAGGGGGATGCGGATAAATGGAAAGGCGAAAGAAAGAATATATATATAATATAT
TTCAAATTCCCTTATATATCCAAATATAAAAATATCTAATAAATTAGATGAATATCAAAGAATCTATTGATTTAGTATACCAGA *
gi|6273291|gb|AF191665.1|AF191 0 chrUn 1 60 156M * 0 0 TATACATTAAAGGAGGGGGATGCGGATAAATGGAAAGGCGAAAGAAAGAATATATATATATATATAATATATTT
CAAATTCCCTTATATATCCAAATATAAAAATATCTAATAAATTAGATGAATATCAAAGAATCTATTGATTTAGTGTACCAGA *
gi|6273287|gb|AF191661.1|AF191 0 chrUn 1 60 56M10D90M * 0 0 TATACATTAAAGAAGGGGGATGCGGATAAATGGAAAGGCGAAAGAAAGAATATATAATATATTTCA
AATTTCCTTATATATCCAAATATAAAAATATCTAATAAATTAGATGAATATCAAAGAATCTATTGATTTAGTGTACCAGA*
gi|6273286|gb|AF191660.1|AF191 0 chrUn 1 60 56M10D90M * 0 0 TATACATAAAAGAAGGGGGATGCGGATAAATGGAAAGGCGAAAGAAAGAATATATAATATATTTAT
AATTTCCTTATATATCCAAATATAAAAATATCTAATAAATTAGATGAATATCAAAGAATCTATTGATTTAGTGTACCAGA*
gi|6273290|gb|AF191664.1|AF191 0 chrUn 1 60 60M6D90M * 0 0 TATACATTAAAGGAGGGGGATGCGGATAAATGGAAAGGCGAAAGAAAGAATATATATATAATATAT
TTCAAATTCCCTTATATATCCAAATATAAAAATATCTAATAAATTAGATGAATATCAAAGAATCTATTGATTTAGTGTACCAGA *
```
END_DOC
*/
@Program(name="biostar139647",
description="Convert alignment in Fasta/Clustal format to SAM/BAM file",
biostars= 139647
)
public class Biostar139647 extends Launcher
{
private static final Logger LOG = Logger.build(Biostar139647.class).make();
@Parameter(names={"-o","--output"},description="Output file. Optional . Default: stdout")
private File outputFile = null;
@Parameter(names={"-R","--refname"},description="reference name. Optional")
private String REF = "chrUn";
@ParametersDelegate
private WritingBamArgs writingBamArgs=new WritingBamArgs();
private static final char CLIPPING=' ';
private int align_length=0;
private Map<String, AlignSequence> sample2sequence=new HashMap<String, AlignSequence>();
//private AbstractSequence consensus=null;
private enum Format{None,Clustal,Fasta};
private abstract class AbstractSequence
{
abstract char at(int index);
@Override
public String toString()
{
StringBuilder b=new StringBuilder(align_length);
for(int i=0;i< align_length;++i) b.append(at(i));
return b.toString();
}
}
private abstract class Sequence extends AbstractSequence
{
StringBuilder seq=new StringBuilder();
char at(int index)
{
return(index< 0 || index >=seq.length()?CLIPPING:Character.toUpperCase(seq.charAt(index)));
}
}
private class AlignSequence extends Sequence
{
String name;
}
@Override
public int doWork(final List<String> args) {
SAMFileWriter w=null;
LineIterator r=null;
SAMFileWriterFactory sfwf=null;
try
{
final String filename = super.oneFileOrNull(args);
if(filename==null)
{
LOG.info("Reading from stdin");
r=IOUtils.openStreamForLineIterator(stdin());
}
else
{
LOG.info("Reading from "+filename);
r=IOUtils.openURIForLineIterator(filename);
}
Format format=Format.None;
while(r.hasNext() && format==Format.None)
{
String line=r.peek();
if( line.trim().isEmpty()) { r.next(); continue;}
if(line.startsWith("CLUSTAL"))
{
format=Format.Clustal;
r.next();//consume
break;
}
else if(line.startsWith(">"))
{
format=Format.Fasta;
break;
}
else
{
return wrapException("MSA format not recognized in "+line);
}
}
LOG.info("format : "+format);
if(Format.Fasta.equals(format))
{
//this.consensus=new FastaConsensus();
AlignSequence curr=null;
while(r.hasNext())
{
String line=r.next();
if(line.startsWith(">"))
{
curr=new AlignSequence();
curr.name=line.substring(1).trim();
if(sample2sequence.containsKey(curr.name))
{
return wrapException("Sequence ID "+curr.name +" defined twice");
}
sample2sequence.put(curr.name, curr);
}
else if(curr!=null)
{
curr.seq.append(line.trim());
this.align_length=Math.max(this.align_length, curr.seq.length());
}
}
}
else if(Format.Clustal.equals(format))
{
AlignSequence curr=null;
int columnStart=-1;
while(r.hasNext())
{
String line=r.next();
if( line.trim().isEmpty() || line.startsWith("CLUSTAL W"))
{
columnStart=-1;
continue;
}
if(line.charAt(0)==' ')
{
if(columnStart==-1)
{
return wrapException("illegal consensus line for "+line);
}
}
else
{
if(columnStart==-1)
{
columnStart=line.indexOf(' ');
if(columnStart==-1)
{
return wrapException("no whithespace in "+line);
}
while(columnStart< line.length() && line.charAt(columnStart)==' ')
{
columnStart++;
}
}
String seqname=line.substring(0, columnStart).trim();
curr=this.sample2sequence.get(seqname);
if(curr==null)
{
curr=new AlignSequence();
curr.name=seqname;
this.sample2sequence.put(curr.name, curr);
}
curr.seq.append(line.substring(columnStart));
this.align_length=Math.max(align_length, curr.seq.length());
}
}
}
else
{
return wrapException("Undefined input format");
}
SAMFileHeader header=new SAMFileHeader();
SAMSequenceDictionary dict=new SAMSequenceDictionary();
dict.addSequence(new SAMSequenceRecord(REF,this.align_length));
header.setSortOrder(SortOrder.unsorted);
header.setSequenceDictionary(dict);
SAMProgramRecord pgr=header.createProgramRecord();
pgr.setProgramName(getProgramName());
pgr.setProgramVersion(getVersion());
if(getProgramCommandLine().trim().isEmpty())
{
pgr.setCommandLine("(empty)");
}
else
{
pgr.setCommandLine(getProgramCommandLine());
}
w = this.writingBamArgs.openSAMFileWriter(this.outputFile, header, false);
DefaultSAMRecordFactory samRecordFactory = new DefaultSAMRecordFactory();
for(String seqName: this.sample2sequence.keySet())
{
AlignSequence shortRead = this.sample2sequence.get(seqName);
SAMRecord rec = samRecordFactory.createSAMRecord(header);
rec.setReadName(seqName);
rec.setReadString(shortRead.seq.toString().replaceAll("[\\*\\- ]+", ""));
int start=0;
while(start< shortRead.seq.length() && !Character.isLetter(shortRead.at(start)))
{
start++;
}
rec.setAlignmentStart(start+1);
int end=shortRead.seq.length()-1;
while(end>0 && !Character.isLetter(shortRead.at(end)))
{
--end;
}
//rec.setAlignmentEnd(end+1); not supported
int n=start;
StringBuilder dna=new StringBuilder();
final List<CigarElement> cigars =new ArrayList<>();
while(n<=end )
{
if( !Character.isLetter(shortRead.at(n)))
{
cigars.add(new CigarElement(1,CigarOperator.D));
}
else
{
cigars.add(new CigarElement(1,CigarOperator.M));
dna.append(shortRead.at(n));
}
n++;
}
//simplify cigar string
n=0;
while(n+1< cigars.size())
{
CigarElement c1= cigars.get(n);
CigarElement c2= cigars.get(n+1);
if(c1.getOperator().equals(c2.getOperator()))
{
cigars.set(n, new CigarElement(c1.getLength()+c2.getLength(), c1.getOperator()));
cigars.remove(n+1);
}
else
{
++n;
}
}
rec.setReadPairedFlag(false);
rec.setMappingQuality(60);
rec.setReferenceName(REF);
rec.setReadString(dna.toString());
rec.setCigar(new Cigar(cigars));
w.addAlignment(rec);
}
LOG.info("Done");
return RETURN_OK;
}
catch(Exception err)
{
return wrapException(err);
}
finally
{
CloserUtil.close(r);
CloserUtil.close(w);
}
}
public static void main(String[] args) {
new Biostar139647().instanceMainWithExit(args);
}
}