/* The MIT License (MIT) Copyright (c) 2015 Pierre Lindenbaum Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. History: * 2015 creation */ package com.github.lindenb.jvarkit.tools.biostar; import htsjdk.samtools.BAMRecordCodec; import htsjdk.samtools.SAMFileHeader; import htsjdk.samtools.SAMFileHeader.SortOrder; import htsjdk.samtools.SAMFileWriter; import htsjdk.samtools.SAMRecord; import htsjdk.samtools.SAMRecordIterator; import htsjdk.samtools.SAMRecordQueryNameComparator; import htsjdk.samtools.SamReader; import htsjdk.samtools.util.BinaryCodec; import htsjdk.samtools.util.CloseableIterator; import htsjdk.samtools.util.CloserUtil; import htsjdk.samtools.util.SortingCollection; import java.io.File; import java.io.IOException; import java.io.InputStream; import java.io.OutputStream; import java.util.Comparator; import java.util.List; import java.util.Random; import com.beust.jcommander.Parameter; import com.beust.jcommander.ParametersDelegate; import com.github.lindenb.jvarkit.util.jcommander.Launcher; import com.github.lindenb.jvarkit.util.jcommander.Program; import com.github.lindenb.jvarkit.util.log.Logger; import com.github.lindenb.jvarkit.util.picard.SAMSequenceDictionaryProgress; /* BEGIN_DOC ## Example ```bash $ java -jar dist/biostar145820.jar -n 10 -o out.bam in.bam ``` END_DOC */ @Program(name="biostar145820",description="subsample BAM to fixed number of alignments.",biostars=145820) public class Biostar145820 extends Launcher { private static final Logger LOG = Logger.build(Biostar145820.class).make(); @Parameter(names={"-o","--output"},description="Output file. Optional . Default: stdout") private File outputFile = null; @Parameter(names={"-n"},description=" number of reads. -1: all reads") private long count=-1L; @ParametersDelegate private WritingSortingCollection writingSortingCollection = new WritingSortingCollection(); @ParametersDelegate private WritingBamArgs writingBamArgs = new WritingBamArgs(); private static class RandSamRecord { int rand_index; SAMRecord samRecord; } private static class RandSamRecordComparator implements Comparator<RandSamRecord> { SAMRecordQueryNameComparator secondCompare =new SAMRecordQueryNameComparator(); @Override public int compare(RandSamRecord o1, RandSamRecord o2) { long i = (long)o1.rand_index - (long)o2.rand_index; if(i!=0L) return (i<0?-1:1); return secondCompare.compare(o1.samRecord, o2.samRecord); } } private static class RandSamRecordCodec implements SortingCollection.Codec<RandSamRecord> { private final BinaryCodec binaryCodec = new BinaryCodec(); private BAMRecordCodec bamRecordCodec; private SAMFileHeader header; RandSamRecordCodec(SAMFileHeader header) { this.header=header; this.bamRecordCodec=new BAMRecordCodec(this.header); } @Override public RandSamRecord decode() { int r; try { r = this.binaryCodec.readInt(); } catch (Exception e) { return null; } RandSamRecord o =new RandSamRecord(); o.rand_index=r; o.samRecord = this.bamRecordCodec.decode(); return o; } @Override public void encode(RandSamRecord val) { this.binaryCodec.writeInt(val.rand_index); this.bamRecordCodec.encode(val.samRecord); } @Override public void setInputStream(InputStream is) { this.binaryCodec.setInputStream(is); this.bamRecordCodec.setInputStream(is); } @Override public void setOutputStream(OutputStream os) { this.binaryCodec.setOutputStream(os); this.bamRecordCodec.setOutputStream(os); } @Override public RandSamRecordCodec clone() { return new RandSamRecordCodec(this.header); } } @Override public int doWork(final List<String> args) { if(this.count<-1L) // -1 == infinite { LOG.error("Bad count:"+count); return -1; } SamReader samReader=null; SAMRecordIterator iter=null; SAMFileWriter samWriter=null; Random random=new Random(); CloseableIterator<RandSamRecord> iter2=null; try { samReader = super.openSamReader(oneFileOrNull(args)); final SAMFileHeader header=samReader.getFileHeader().clone(); header.setSortOrder(SortOrder.unsorted); header.addComment("Processed with "+getProgramName()+" : "+getProgramCommandLine()); samWriter = writingBamArgs.openSAMFileWriter(outputFile, header, true); iter=samReader.iterator(); SAMSequenceDictionaryProgress progress=new SAMSequenceDictionaryProgress(samReader.getFileHeader().getSequenceDictionary()); SortingCollection<RandSamRecord> sorter=SortingCollection.newInstance( RandSamRecord.class, new RandSamRecordCodec(header), new RandSamRecordComparator(), this.writingSortingCollection.getMaxRecordsInRam(), this.writingSortingCollection.getTmpDirectories() ); sorter.setDestructiveIteration(true); while(iter.hasNext()) { RandSamRecord r=new RandSamRecord(); r.rand_index = random.nextInt(); r.samRecord = progress.watch(iter.next()); sorter.add(r); } iter.close();iter=null; sorter.doneAdding(); iter2=sorter.iterator(); if(count==-1) { while(iter2.hasNext()) { samWriter.addAlignment(iter2.next().samRecord); } } else { while(iter2.hasNext() && count>0) { samWriter.addAlignment(iter2.next().samRecord); count--; } } iter2.close();iter2=null; sorter.cleanup(); progress.finish(); } catch (Exception e) { LOG.error(e); return -1; } finally { CloserUtil.close(iter); CloserUtil.close(iter2); CloserUtil.close(samReader); CloserUtil.close(samWriter); } return 0; } public static void main(String[] args) throws IOException { new Biostar145820().instanceMainWithExit(args); } }