/* The MIT License (MIT) Copyright (c) 2015 Pierre Lindenbaum Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. History: * 2015 creation */ package com.github.lindenb.jvarkit.tools.misc; import htsjdk.samtools.BAMRecordCodec; import htsjdk.samtools.SAMFileHeader; import htsjdk.samtools.SAMFileHeader.SortOrder; import htsjdk.samtools.SAMFileWriter; import htsjdk.samtools.SAMRecord; import htsjdk.samtools.SAMRecordIterator; import htsjdk.samtools.SAMRecordQueryNameComparator; import htsjdk.samtools.SamReader; import htsjdk.samtools.util.CloseableIterator; import htsjdk.samtools.util.CloserUtil; import htsjdk.samtools.util.SortingCollection; import java.io.File; import java.io.IOException; import java.util.Comparator; import java.util.List; import com.github.lindenb.jvarkit.util.picard.SAMSequenceDictionaryProgress; import com.beust.jcommander.Parameter; import com.beust.jcommander.ParametersDelegate; import com.github.lindenb.jvarkit.util.jcommander.Launcher; import com.github.lindenb.jvarkit.util.jcommander.Program; import com.github.lindenb.jvarkit.util.log.Logger; /** BEGIN_DOC ## Example ```bash $ java -jar dist/sortsamrefname.jar /commun/data/packages/samtools/1.2/samtools/examples/toy.sam 2> /dev/null @HD VN:1.4 SO:unsorted @SQ SN:ref LN:45 @SQ SN:ref2 LN:40 @CO SortSamRefName 1c7bc5e674136947586779a2aac53e576db4a67f /commun/data/packages/samtools/1.2/samtools/examples/toy.sam r001 83 ref 37 30 9M = 7 -39 CAGCGCCAT * r001 163 ref 7 30 8M4I4M1D3M = 37 39 TTAGATAAAGAGGATACTG * XX:B:S,12561,2,20,112 r002 0 ref 9 30 1S2I6M1P1I1P1I4M2I * 0 0 AAAAGATAAGGGATAAA * r003 0 ref 9 30 5H6M * 0 0 AGCTAA * r003 16 ref 29 30 6H5M * 0 0 TAGGC * r004 0 ref 16 30 6M14N1I5M * 0 0 ATAGCTCTCAGC * x1 0 ref2 1 30 20M * 0 0 AGGTTTTATAAAACAAATAA ???????????????????? x2 0 ref2 2 30 21M * 0 0 GGTTTTATAAAACAAATAATT ????????????????????? x3 0 ref2 6 30 9M4I13M * 0 0 TTATAAAACAAATAATTAAGTCTACA ?????????????????????????? x4 0 ref2 10 30 25M * 0 0 CAAATAATTAAGTCTACAGAGCAAC ????????????????????????? x5 0 ref2 12 30 24M * 0 0 AATAATTAAGTCTACAGAGCAACT ???????????????????????? x6 0 ref2 14 30 23M * 0 0 TAATTAAGTCTACAGAGCAACTA ??????????????????????? ``` END_DOC */ @Program(name="sortsamrefname",description="Sort a BAM of contig and then on name") public class SortSamRefName extends Launcher { private static final Logger LOG = Logger.build(SortSamRefName.class).make(); @Parameter(names={"-o","--output"},description="Output file. Optional . Default: stdout") private File outputFile = null; @ParametersDelegate private WritingSortingCollection writingSortingCollection=new WritingSortingCollection(); @ParametersDelegate private WritingBamArgs writingBamArgs=new WritingBamArgs(); private static class RefNameComparator implements Comparator<SAMRecord> { private final SAMRecordQueryNameComparator nameCmp=new SAMRecordQueryNameComparator(); @Override public int compare(final SAMRecord o1, final SAMRecord o2) { final int refIndex1 = o1.getReferenceIndex(); final int refIndex2 = o2.getReferenceIndex(); final int cmp = refIndex1 - refIndex2; if (cmp != 0) { if (refIndex1 == -1 ) return 1; if (refIndex2 == -1 ) return -1; return cmp; } return nameCmp.compare(o1, o2); } } public SortSamRefName() { } @Override public int doWork(List<String> args) { SamReader in=null; SAMFileWriter out=null; SAMRecordIterator iter=null; CloseableIterator<SAMRecord> iter2=null; SortingCollection<SAMRecord> sorter=null; try { in = openSamReader(oneFileOrNull(args)); final SAMFileHeader header= in.getFileHeader(); final BAMRecordCodec bamRecordCodec=new BAMRecordCodec(header); final RefNameComparator refNameComparator=new RefNameComparator(); sorter =SortingCollection.newInstance( SAMRecord.class, bamRecordCodec, refNameComparator, this.writingSortingCollection.getMaxRecordsInRam(), this.writingSortingCollection.getTmpDirectories() ); sorter.setDestructiveIteration(true); final SAMSequenceDictionaryProgress progress=new SAMSequenceDictionaryProgress(header); iter = in.iterator(); while(iter.hasNext()) { sorter.add( progress.watch(iter.next())); } in.close();in=null; sorter.doneAdding(); final SAMFileHeader header2=header.clone(); header2.addComment(getProgramName()+" "+getVersion()+" "+getProgramCommandLine()); header2.setSortOrder(SortOrder.unsorted); out = this.writingBamArgs.openSAMFileWriter(outputFile,header2, true); iter2 = sorter.iterator(); while(iter2.hasNext()) { out.addAlignment(iter2.next()); } out.close();out=null; sorter.cleanup(); progress.finish(); LOG.info("done"); return RETURN_OK; } catch(Exception err) { LOG.error(err); return -1; } finally { CloserUtil.close(iter2); CloserUtil.close(iter); CloserUtil.close(out); CloserUtil.close(in); } } public static void main(final String[] args) throws IOException { new SortSamRefName().instanceMainWithExit(args); } }