/* Copyright (c) 2009 The Regents of the University of California. All rights reserved. Permission is hereby granted, without written agreement and without license or royalty fees, to use, copy, modify, and distribute this software and its documentation for any purpose, provided that the above copyright notice and the following two paragraphs appear in all copies of this software. IN NO EVENT SHALL THE UNIVERSITY OF CALIFORNIA BE LIABLE TO ANY PARTY FOR DIRECT, INDIRECT, SPECIAL, INCIDENTAL, OR CONSEQUENTIAL DAMAGES ARISING OUT OF THE USE OF THIS SOFTWARE AND ITS DOCUMENTATION, EVEN IF THE UNIVERSITY OF CALIFORNIA HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. THE UNIVERSITY OF CALIFORNIA SPECIFICALLY DISCLAIMS ANY WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. THE SOFTWARE PROVIDED HEREUNDER IS ON AN "AS IS" BASIS, AND THE UNIVERSITY OF CALIFORNIA HAS NO OBLIGATION TO PROVIDE MAINTENANCE, SUPPORT, UPDATES, ENHANCEMENTS, OR MODIFICATIONS.. */ package org.clothocad.format.concatenateformat; import java.awt.Color; import java.util.ArrayList; import org.clothocore.api.data.Annotation; import org.clothocore.api.data.Feature; import org.clothocore.api.data.NucSeq; import org.clothocore.api.data.Part; import org.clothocore.api.data.Person; import org.clothocore.api.data.Plasmid; import org.clothocore.api.data.Vector; import org.clothocore.api.plugin.ClothoFormat; /** * Format Interface is what a ClothoFormat plugin must implement * @author jcanderson */ public class connect implements ClothoFormat { @Override public void init() { } @Override public boolean checkPart(Part p) { return true; } @Override public boolean checkVector(Vector v) { return true; } @Override public boolean checkComposite(ArrayList<Part> composition, Object additionalRequirements) { //A BglBrick composite Part should have at least 2 parents if(composition.size()<2) { return false; } return true; } @Override public boolean checkPlasmid(Part p, Vector v, Object additionalRequirements) { //Confirm that the Part and Vector exist if(p!=null && v!=null) { return true; } return false; } @Override public NucSeq generateCompositeSequence(ArrayList<Part> composition, Object additionalRequirements) { StringBuffer sb = new StringBuffer(); for(Part p: composition) { sb.append(p.getSeq().getSeq()); } NucSeq outnuc = new NucSeq(sb.toString()); outnuc.setTransient(); int offset = 0; for(Part p: composition) { String parseq = p.getSeq().getSeq(); for(Annotation ann : p.getSeq().getAnnotations()) { Feature afeature = ann.getFeature(); String name = ann.getName(); Color forColor = ann.getForwardColor(); Color revColor = ann.getReverseColor(); int Start = offset + ann.getStart(); int End = offset + ann.getEnd(); Person user = ann.getAuthor(); boolean plusstrandtrue = ann.isForwardStrand(); String symbol = ann.getSymbol(); Annotation newann; if(afeature==null) { newann = new Annotation(name, outnuc, forColor, forColor, Start, End, user, plusstrandtrue, symbol); newann.setTransient(); } else { newann = new Annotation(afeature, outnuc, forColor, forColor, Start, End, user, plusstrandtrue, symbol); newann.setTransient(); } System.out.println("Generated annotation: " + newann.getName()); } offset += parseq.length(); } return outnuc; } @Override public NucSeq generatePlasmidSequence(Plasmid p) { String out = ""; out+=p.getPart().getSeq().getSeq(); out+=p.getVector().getSeq().getSeq(); NucSeq outnuc = new NucSeq(out); outnuc.setTransient(); for(Annotation ann : p.getPart().getSeq().getAnnotations()) { Feature afeature = ann.getFeature(); String name = ann.getName(); Color forColor = ann.getForwardColor(); Color revColor = ann.getReverseColor(); int Start = ann.getStart(); int End = ann.getEnd(); Person user = ann.getAuthor(); boolean plusstrandtrue = ann.isForwardStrand(); String symbol = ann.getSymbol(); Annotation newann; if(afeature==null) { newann = new Annotation(name, outnuc, forColor, forColor, Start, End, user, plusstrandtrue, symbol); newann.setTransient(); } else { newann = new Annotation(afeature, outnuc, forColor, revColor, Start, End, user, plusstrandtrue, symbol); newann.setTransient(); } System.out.println("Generated annotation: " + newann.getName()); } String parseq = p.getPart().getSeq().getSeq(); int offset = parseq.length(); for(Annotation ann : p.getVector().getSeq().getAnnotations()) { Feature afeature = ann.getFeature(); String name = ann.getName(); Color forColor = ann.getForwardColor(); Color revColor = ann.getReverseColor(); int Start = offset + ann.getStart(); int End = offset + ann.getEnd(); Person user = ann.getAuthor(); boolean plusstrandtrue = ann.isForwardStrand(); String symbol = ann.getSymbol(); Annotation newann; if(afeature==null) { newann = new Annotation(name, outnuc, forColor, forColor, Start, End, user, plusstrandtrue, symbol); newann.setTransient(); } else { newann = new Annotation(afeature, outnuc, forColor, revColor, Start, End, user, plusstrandtrue, symbol); newann.setTransient(); } System.out.println("Generated annotation: " + newann.getName()); } return outnuc; } @Override public NucSeq generateSequencingRegion(Plasmid p) { return p.getPart().getSeq(); } }