/*
Copyright (C) 2003 EBI, GRL
This library is free software; you can redistribute it and/or
modify it under the terms of the GNU Lesser General Public
License as published by the Free Software Foundation; either
version 2.1 of the License, or (at your option) any later version.
This library is distributed in the hope that it will be useful,
but WITHOUT ANY WARRANTY; without even the implied warranty of
MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU
Lesser General Public License for more details.
You should have received a copy of the GNU Lesser General Public
License along with this library; if not, write to the Free Software
Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA
*/
package org.ensembl.mart.lib;
import java.io.IOException;
import java.io.OutputStream;
import java.sql.Connection;
import java.sql.ResultSet;
import java.sql.ResultSetMetaData;
import java.sql.SQLException;
import java.util.ArrayList;
import java.util.Hashtable;
import java.util.Iterator;
import java.util.List;
import java.util.TreeMap;
import java.util.logging.Level;
import java.util.logging.Logger;
import org.ensembl.mart.util.FormattedSequencePrintStream;
import org.ensembl.util.SequenceUtil;
/**
* Outputs peptide sequence in one of the supported output format
*
* @author <a href="mailto:dlondon@ebi.ac.uk">Darin London</a>
* @author <a href="mailto:craig@ebi.ac.uk">Craig Melsopp</a>
* @see FormatSpec for supported output formats
*/
public final class PeptideSeqQueryRunner extends BaseSeqQueryRunner {
private final String LOCATIONS = "locations";
private Logger logger = Logger.getLogger(PeptideSeqQueryRunner.class.getName());
/**
* Constructs a PeptideSeqQueryRunner object to execute a Query
* and print Peptide Sequences
*
* @param query a Query Object
* @param format a FormatSpec object
* @param os an OutputStream object
*/
public PeptideSeqQueryRunner(Query query, FormatSpec format, OutputStream os) {
super(query);
this.format = format;
this.osr = new FormattedSequencePrintStream(maxColumnLen, os, true);
//autoflush true
switch (format.getFormat()) {
case FormatSpec.TABULATED :
this.separator = format.getSeparator();
this.seqWriter = tabulatedWriter;
break;
case FormatSpec.FASTA :
this.separator = "|";
this.seqWriter = fastaWriter;
break;
}
}
protected void updateQuery() {
Attribute[] exportable = query.getSequenceDescription().getFinalLink();
queryID = exportable[0].getField();
qualifiedQueryID = exportable[0].getTableConstraint()+"."+queryID;
chrField = exportable[1].getField();
coordStart = exportable[2].getField();
coordEnd = exportable[3].getField();
strandField = exportable[4].getField();
rankField = exportable[5].getField();
}
protected void processResultSet(Connection conn, ResultSet rs) throws IOException, SQLException {
if (queryIDindex < 0) {
ResultSetMetaData rmeta = rs.getMetaData();
// process columnNames for required attribute indices
for (int i = 1, nColumns = rmeta.getColumnCount(); i <= nColumns; ++i) {
String column = rmeta.getColumnName(i);
if (column.equals(queryID) && queryIDindex < 0)
queryIDindex = i;
else if (column.equals(rankField) && rankIndex < 0)
rankIndex = i;
else if (column.equals(coordStart) && startIndex < 0)
startIndex = i;
else if (column.equals(coordEnd) && endIndex < 0)
endIndex = i;
else if (column.equals(chrField) && chromIndex < 0)
chromIndex = i;
else if (column.equals(strandField) && strandIndex < 0)
strandIndex = i;
else
if (!otherIndices.contains(new Integer(i)))
otherIndices.add(new Integer(i));
}
}
while (rs.next()) {
Integer keyID = new Integer(rs.getInt(queryIDindex));
Integer rank = new Integer(rs.getInt(rankIndex));
if ( keyID.intValue() != lastID ) {
if ( lastID > -1 ) {
//This is not the first ID in a batch, process the previous ID sequences
seqWriter.writeSequences(new Integer(lastID), conn);
}
//refresh the iDs TreeMap
iDs = new TreeMap();
lastIDRowsProcessed = 0; // refresh for the new ID
Hashtable atts = new Hashtable();
atts.put(LOCATIONS, new TreeMap());
iDs.put(keyID, atts);
}
Hashtable atts = (Hashtable) iDs.get(keyID);
int start = rs.getInt(startIndex);
if (start > 0) {
// if start is not null, create a new SequenceLocation object from the chr, start, end, and strand
String chr = rs.getString(chromIndex);
int end = rs.getInt(endIndex);
int strand = rs.getInt(strandIndex);
// order the locations by their rank in ascending order
((TreeMap) atts.get(LOCATIONS)).put(rank, new SequenceLocation(chr, start, end, strand));
}
// Rest can be duplicates, or novel values for a given field, collect lists of values for each field
// currindex is now the last index of the DisplayIDs. Increment it, and iterate over the rest of the ResultSet to print the description
for (int i = 0, n = otherIndices.size(); i < n; i++) {
int currindex = ((Integer) otherIndices.get(i)).intValue();
if (rs.getString(currindex) != null) {
String field = attributes[currindex - 1].getField();
if (!fields.contains(field))
fields.add(field);
String value = rs.getString(currindex);
if (atts.containsKey(field)) {
if (!((ArrayList) atts.get(field)).contains(value))
((ArrayList) atts.get(field)).add(value);
} else {
List values = new ArrayList();
values.add(value);
atts.put(field, values);
}
}
}
totalRows++;
totalRowsThisExecute++;
resultSetRowsProcessed++;
lastID = keyID.intValue();
lastIDRowsProcessed++;
}
}
private final SeqWriter tabulatedWriter = new SeqWriter() {
void writeSequences(Integer tranID, Connection conn) throws SequenceException {
try {
Hashtable atts = (Hashtable) iDs.get(tranID);
for (int j = 0, n = fields.size(); j < n; j++) {
if (j > 0)
osr.print(separator);
String field = (String) fields.get(j);
if (atts.containsKey(field)) {
List values = (ArrayList) atts.get(field);
for (int vi = 0; vi < values.size(); vi++) {
if (vi > 0)
osr.print(",");
osr.print((String) values.get(vi));
}
}
}
osr.print(separator);
if (osr.checkError())
throw new IOException();
TreeMap locations = (TreeMap) atts.get(LOCATIONS);
List locbytes = new ArrayList();
int seqLen = 0;
// to collect all sequence before translation
for (Iterator lociter = locations.keySet().iterator(); lociter.hasNext();) {
SequenceLocation loc = (SequenceLocation) locations.get((Integer) lociter.next());
byte[] theseBytes = null;
if (loc.getStrand() < 0)
theseBytes = SequenceUtil.reverseComplement(dna.getSequence(loc.getChr(), loc.getStart(), loc.getEnd()));
else
theseBytes = dna.getSequence(loc.getChr(), loc.getStart(), loc.getEnd());
locbytes.add(theseBytes);
seqLen += theseBytes.length;
}
//iterate through locbytes to fill sequence byte[]
byte[] sequence = new byte[seqLen];
int nextPos = 0;
for (int i = 0, n = locbytes.size(); i < n; i++) {
byte[] thisChunk = (byte[]) locbytes.get(i);
System.arraycopy(thisChunk, 0, sequence, nextPos, thisChunk.length);
nextPos += thisChunk.length;
}
locbytes = null;
osr.write(SequenceUtil.dna2protein(sequence));
osr.print("\n");
if (osr.checkError())
throw new IOException();
} catch (SequenceException e) {
if (logger.isLoggable(Level.WARNING))
logger.warning(e.getMessage());
throw e;
} catch (IOException e) {
if (logger.isLoggable(Level.WARNING))
logger.warning("Couldnt write to OutputStream\n" + e.getMessage());
throw new SequenceException(e);
}
}
};
private final SeqWriter fastaWriter = new SeqWriter() {
void writeSequences(Integer tranID, Connection conn) throws SequenceException {
try {
Hashtable atts = (Hashtable) iDs.get(tranID);
osr.print(">");
if (osr.checkError())
throw new IOException();
for (int j = 0, n = fields.size(); j < n; j++) {
if (j > 0)
osr.print(separator);
String field = (String) fields.get(j);
if (atts.containsKey(field)) {
List values = (ArrayList) atts.get(field);
for (int vi = 0; vi < values.size(); vi++) {
if (vi > 0)
osr.print(",");
osr.print((String) values.get(vi));
}
}
}
osr.print("\n");
if (osr.checkError())
throw new IOException();
TreeMap locations = (TreeMap) atts.get(LOCATIONS);
List locbytes = new ArrayList();
int seqLen = 0;
// to collect all sequence before translation
for (Iterator lociter = locations.keySet().iterator(); lociter.hasNext();) {
SequenceLocation loc = (SequenceLocation) locations.get((Integer) lociter.next());
byte[] theseBytes = null;
if (loc.getStrand() < 0)
theseBytes = SequenceUtil.reverseComplement(dna.getSequence(loc.getChr(), loc.getStart(), loc.getEnd()));
else
theseBytes = dna.getSequence(loc.getChr(), loc.getStart(), loc.getEnd());
locbytes.add(theseBytes);
seqLen += theseBytes.length;
}
//iterate through locbytes to fill sequence byte[]
byte[] sequence = new byte[seqLen];
int nextPos = 0;
for (int i = 0, n = locbytes.size(); i < n; i++) {
byte[] thisChunk = (byte[]) locbytes.get(i);
System.arraycopy(thisChunk, 0, sequence, nextPos, thisChunk.length);
nextPos += thisChunk.length;
}
locbytes = null;
osr.writeSequence(SequenceUtil.dna2protein(sequence));
osr.print("\n");
osr.resetColumnCount();
if (osr.checkError())
throw new IOException();
} catch (SequenceException e) {
if (logger.isLoggable(Level.WARNING))
logger.warning(e.getMessage());
throw e;
} catch (IOException e) {
if (logger.isLoggable(Level.WARNING))
logger.warning("Couldnt write to OutputStream\n" + e.getMessage());
throw new SequenceException(e);
}
}
};
}