/* Copyright (C) 2003 EBI, GRL This library is free software; you can redistribute it and/or modify it under the terms of the GNU Lesser General Public License as published by the Free Software Foundation; either version 2.1 of the License, or (at your option) any later version. This library is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU Lesser General Public License for more details. You should have received a copy of the GNU Lesser General Public License along with this library; if not, write to the Free Software Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA */ package org.ensembl.mart.lib; import java.io.IOException; import java.io.OutputStream; import java.sql.Connection; import java.sql.ResultSet; import java.sql.ResultSetMetaData; import java.sql.SQLException; import java.util.ArrayList; import java.util.Hashtable; import java.util.Iterator; import java.util.List; import java.util.TreeMap; import java.util.logging.Level; import java.util.logging.Logger; import org.ensembl.mart.util.FormattedSequencePrintStream; import org.ensembl.util.SequenceUtil; /** * Outputs Cdna sequence in one of the supported output formats * * @author <a href="mailto:dlondon@ebi.ac.uk">Darin London</a> * @author <a href="mailto:craig@ebi.ac.uk">Craig Melsopp</a> * @see FormatSpec for supported output formats */ public final class CdnaSeqQueryRunner extends BaseSeqQueryRunner { private final String LOCATIONS = "locations"; private Logger logger = Logger.getLogger(CdnaSeqQueryRunner.class.getName()); /** * Constructs a CdnaSeqQueryRunner object to execute a Query * and print output of cdna Sequences * * @param query a Query Object * @param format a FormatSpec object * @param os an OutputStream object */ public CdnaSeqQueryRunner(Query query, FormatSpec format, OutputStream os) { super(query); this.format = format; this.osr = new FormattedSequencePrintStream(maxColumnLen, os, true); //autoflush true switch (format.getFormat()) { case FormatSpec.TABULATED : this.separator = format.getSeparator(); this.seqWriter = tabulatedWriter; break; case FormatSpec.FASTA : this.separator = "|"; this.seqWriter = fastaWriter; break; } } protected void updateQuery() { //we no longer need to actually update the query, as seqDescription //has already done that. We just need to find out what the exportable //is, eg, the key, start, end, and strand Attribute[] exportable = query.getSequenceDescription().getFinalLink(); queryID = exportable[0].getField(); qualifiedQueryID = exportable[0].getTableConstraint()+"."+queryID; chrField = exportable[1].getField(); coordStart = exportable[2].getField(); coordEnd = exportable[3].getField(); strandField = exportable[4].getField(); rankField = exportable[5].getField(); } protected void processResultSet(Connection conn, ResultSet rs) throws IOException, SQLException { if (queryIDindex < 0) { ResultSetMetaData rmeta = rs.getMetaData(); // process columnNames for required attribute indices for (int i = 1, nColumns = rmeta.getColumnCount(); i <= nColumns; ++i) { String column = rmeta.getColumnName(i); if (column.equals(queryID) && queryIDindex < 0) queryIDindex = i; else if (column.equals(rankField) && rankIndex < 0) rankIndex = i; else if (column.equals(coordStart) && startIndex < 0) startIndex = i; else if (column.equals(coordEnd) && endIndex < 0) endIndex = i; else if (column.equals(chrField) && chromIndex < 0) chromIndex = i; else if (column.equals(strandField) && strandIndex < 0) strandIndex = i; else otherIndices.add(new Integer(i)); } } while (rs.next()) { Integer keyID = new Integer(rs.getInt(queryIDindex)); Integer rank = new Integer(rs.getInt(rankIndex)); if ( keyID.intValue() != lastID ) { if ( lastID > -1 ) { //This is not the first ID in a batch, process the previous ID sequences seqWriter.writeSequences(new Integer(lastID), conn); } //refresh the iDs TreeMap iDs = new TreeMap(); lastIDRowsProcessed = 0; // refresh for the new ID Hashtable atts = new Hashtable(); atts.put(LOCATIONS, new TreeMap()); iDs.put(keyID, atts); } Hashtable tranatts = (Hashtable) iDs.get(keyID); int start = rs.getInt(startIndex); if (start > 0) { // if start is not null, create a new SequenceLocation object from the chr, start, end, and strand String chr = rs.getString(chromIndex); int end = rs.getInt(endIndex); int strand = rs.getInt(strandIndex); // order the locations by their rank in ascending order ((TreeMap) tranatts.get(LOCATIONS)).put(rank, new SequenceLocation(chr, start, end, strand)); } // Rest can be duplicates, or novel values for a given field, collect lists of values for each field // currindex is now the last index of the DisplayIDs. Increment it, and iterate over the rest of the ResultSet to print the description for (int i = 0, n = otherIndices.size(); i < n; i++) { int currindex = ((Integer) otherIndices.get(i)).intValue(); if (rs.getString(currindex) != null) { String field = attributes[currindex - 1].getField(); if (!fields.contains(field)) fields.add(field); String value = rs.getString(currindex); if (tranatts.containsKey(field)) { if (!((ArrayList) tranatts.get(field)).contains(value)) ((ArrayList) tranatts.get(field)).add(value); } else { List values = new ArrayList(); values.add(value); tranatts.put(field, values); } } } totalRows++; totalRowsThisExecute++; resultSetRowsProcessed++; lastID = keyID.intValue(); lastIDRowsProcessed++; } } private final SeqWriter tabulatedWriter = new SeqWriter() { void writeSequences(Integer tranID, Connection conn) throws SequenceException { try { Hashtable tranatts = (Hashtable) iDs.get(tranID); if (osr.checkError()) throw new IOException(); for (int j = 0, n = fields.size(); j < n; j++) { if (j > 0) osr.print(separator); String field = (String) fields.get(j); if (tranatts.containsKey(field)) { List values = (ArrayList) tranatts.get(field); for (int vi = 0; vi < values.size(); vi++) { if (vi > 0) osr.print(","); osr.print((String) values.get(vi)); } } } osr.print(separator); if (osr.checkError()) throw new IOException(); TreeMap locations = (TreeMap) tranatts.get(LOCATIONS); for (Iterator lociter = locations.keySet().iterator(); lociter.hasNext();) { SequenceLocation loc = (SequenceLocation) locations.get((Integer) lociter.next()); if (loc.getStrand() < 0) osr.write(SequenceUtil.reverseComplement(dna.getSequence(loc.getChr(), loc.getStart(), loc.getEnd()))); else osr.write(dna.getSequence(loc.getChr(), loc.getStart(), loc.getEnd())); } osr.print("\n"); if (osr.checkError()) throw new IOException(); } catch (SequenceException e) { if (logger.isLoggable(Level.WARNING)) logger.warning(e.getMessage()); throw e; } catch (IOException e) { if (logger.isLoggable(Level.WARNING)) logger.warning("Couldnt write to OutputStream\n" + e.getMessage()); throw new SequenceException(e); } } }; private final SeqWriter fastaWriter = new SeqWriter() { void writeSequences(Integer tranID, Connection conn) throws SequenceException { try { Hashtable tranatts = (Hashtable) iDs.get(tranID); // write the header, starting with the displayID osr.print(">"); if (osr.checkError()) throw new IOException(); for (int j = 0, n = fields.size(); j < n; j++) { if (j > 0) osr.print(separator); String field = (String) fields.get(j); if (tranatts.containsKey(field)) { List values = (ArrayList) tranatts.get(field); for (int vi = 0; vi < values.size(); vi++) { if (vi > 0) osr.print(","); osr.print((String) values.get(vi)); } } else osr.print(field + "= "); } osr.print("\n"); if (osr.checkError()) throw new IOException(); TreeMap locations = (TreeMap) tranatts.get(LOCATIONS); for (Iterator lociter = locations.keySet().iterator(); lociter.hasNext();) { SequenceLocation loc = (SequenceLocation) locations.get((Integer) lociter.next()); if (loc.getStrand() < 0) osr.writeSequence(SequenceUtil.reverseComplement(dna.getSequence(loc.getChr(), loc.getStart(), loc.getEnd()))); else osr.writeSequence(dna.getSequence(loc.getChr(), loc.getStart(), loc.getEnd())); } osr.print("\n"); osr.resetColumnCount(); if (osr.checkError()) throw new IOException(); } catch (SequenceException e) { if (logger.isLoggable(Level.WARNING)) logger.warning(e.getMessage()); throw e; } catch (IOException e) { if (logger.isLoggable(Level.WARNING)) logger.warning("Couldnt write to OutputStream\n" + e.getMessage()); throw new SequenceException(e); } } }; }