// Copyright (C) 2011-2012 CRS4. // // This file is part of Seal. // // Seal is free software: you can redistribute it and/or modify it // under the terms of the GNU General Public License as published by the Free // Software Foundation, either version 3 of the License, or (at your option) // any later version. // // Seal is distributed in the hope that it will be useful, but // WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY // or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License // for more details. // // You should have received a copy of the GNU General Public License along // with Seal. If not, see <http://www.gnu.org/licenses/>. package it.crs4.seal.recab; import it.crs4.seal.common.AbstractTaggedMapping; public class CycleCovariate implements Covariate { private int readNum = -1; private int readLength = -1; private boolean forwardStrand; public void applyToMapping(AbstractTaggedMapping m) { // is isRead2 is set, then read 2. All other cases (e.g. unpaired), then read 1 readNum = m.isRead2() ? 2 : 1; readLength = m.getLength(); forwardStrand = !m.isOnReverse(); } public String getValue(int pos) { if (readLength < 0) throw new RuntimeException("BUG! readLength == " + readLength + ". applyToMapping not called before CycleCovariate.getValue"); else if (readNum < 0) throw new RuntimeException("BUG! readNum == " + readNum + ". applyToMapping not called before CycleCovariate.getValue"); else if (pos < 0 || pos >= readLength) throw new IndexOutOfBoundsException("pos " + pos + " is out of read boundaries [0," + readLength + ")"); int cycle; if (forwardStrand) cycle = pos + 1; else cycle = readLength - pos; if (readNum == 2) cycle *= -1; return String.valueOf(cycle); } }