/*
* Copyright (c) 2003-2012 Fred Hutchinson Cancer Research Center
*
* Licensed under the Apache License, Version 2.0 (the "License");
* you may not use this file except in compliance with the License.
* You may obtain a copy of the License at
*
* http://www.apache.org/licenses/LICENSE-2.0
*
* Unless required by applicable law or agreed to in writing, software
* distributed under the License is distributed on an "AS IS" BASIS,
* WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
* See the License for the specific language governing permissions and
* limitations under the License.
*/
package org.fhcrc.cpl.viewer.ms2.commandline;
import org.fhcrc.cpl.toolbox.commandline.arguments.ArgumentValidationException;
import org.fhcrc.cpl.toolbox.commandline.arguments.CommandLineArgumentDefinition;
import org.fhcrc.cpl.toolbox.commandline.arguments.FileToWriteArgumentDefinition;
import org.fhcrc.cpl.toolbox.proteomics.commandline.arguments.FastaFileArgumentDefinition;
import org.fhcrc.cpl.toolbox.commandline.CommandLineModule;
import org.fhcrc.cpl.toolbox.commandline.CommandLineModuleExecutionException;
import org.fhcrc.cpl.viewer.commandline.modules.BaseViewerCommandLineModuleImpl;
import org.apache.log4j.Logger;
import org.fhcrc.cpl.toolbox.proteomics.Protein;
import java.io.*;
/**
* Creates a forward-reverse fasta file from an input fasta file
*/
public class ReverseFastaCLM extends BaseViewerCommandLineModuleImpl
implements CommandLineModule
{
protected static Logger _log = Logger.getLogger(ReverseFastaCLM.class);
protected File outFile = null;
protected Protein[] forwardProteins = null;
public ReverseFastaCLM()
{
init();
}
protected void init()
{
mCommandName = "reversefasta";
mShortDescription = "";
mHelpMessage = "append a reversed version of a fasta file to a fasta file";
CommandLineArgumentDefinition[] argDefs =
{
new FastaFileArgumentDefinition(
CommandLineArgumentDefinition.UNNAMED_PARAMETER_VALUE_ARGUMENT,true, null),
new FileToWriteArgumentDefinition("out", true, null)
};
addArgumentDefinitions(argDefs);
}
public void assignArgumentValues()
throws ArgumentValidationException
{
forwardProteins = (Protein[]) this.getUnnamedArgumentValue();
outFile = getFileArgumentValue("out");
}
/**
* do the actual work
*/
public void execute() throws CommandLineModuleExecutionException
{
try
{
PrintWriter outPW = new PrintWriter(outFile);
for (Protein protein : forwardProteins)
{
printProtein(protein, false, outPW);
}
for (Protein protein : forwardProteins)
{
printProtein(protein, true, outPW);
}
outPW.flush();
outPW.close();
}
catch (Exception e)
{
throw new CommandLineModuleExecutionException(e);
}
}
/**
* Print the protein in FASTA format, reversing sequence and adjusting header if reverse is specified
* @param protein
* @param reverse
* @param outPW
*/
protected void printProtein(Protein protein, boolean reverse, PrintWriter outPW)
{
outPW.print(">");
if (reverse)
outPW.print("rev_");
outPW.println(protein.getHeader());
String forwardSequence = protein.getSequenceAsString();
for (int i=0; i<forwardSequence.length(); i++)
{
int indexToPrint = i;
if (reverse)
indexToPrint = forwardSequence.length() - i - 1;
outPW.print(forwardSequence.charAt(indexToPrint));
if ((i%80 == 79 && i > 0) ||
i == forwardSequence.length()-1)
outPW.println();
}
}
}